Albano González

Chemical and microphysical properties of marine stratiform cloud in the North Atlantic

The chemical and microphysical properties of marine stratiform cloud were measured at a ridgetop elevation of 992 m above mean sea level (AMSL) on Tenerife in the Canary Islands in the eastern North Atlantic during the summers of 1995 and 1996. The results show an inverse relationship between hourly-averaged cloud droplet diameter and droplet number concentration, which ranged from 116 to 1355 cm−3. Strong relationships were observed between droplet number and equivalent clear air concentrations of non-sea-salt sulfate, nitrate, and elemental carbon in the droplets. Droplet sizes inferred from radiances measured by satellite for clouds offshore and upwind agreed with droplet sizes derived for clouds over the mountain sampling site, and also with those measured in cloud 4–5 hours later. Estimated cloud short-wave radiative forcing was enhanced by 8% in radiative model studies of polluted versus clean clouds with droplet concentrations of 786 and 127 cm−3 and droplet effective radii of 6 and 10 μm, respectively.

Remote Sensing of Water Cloud Parameters Using Neural Networks

Abstract In this work a method for determining the micro- and macrophysical properties of oceanic stratocumulus clouds is presented. It is based on the inversion of a radiative transfer model that computes the albedos and brightness temperatures in the NOAA Advanced Very High Resolution Radiometer (AVHRR) channels. This inversion is performed using artificial neural networks (ANNs), which are trained and optimized by genetic algorithms to fit theoretical computations. A detailed study of the ANN parameters and training algorithms demonstrates the convenience of using the “backpropagation with momentum” method. The proposed retrieval method is applied to daytime and nighttime imagery and was validated using ground data collected in Tenerife (Canary Islands), obtaining a good agreement.

Stratocumulus properties retrieval method from NOAA-AVHRR data based on the discretization of cloud parameters

A method is presented for determining the optical thickness, effective droplet radius and temperature of oceanic stratocumulus clouds from NOAA-AVHRR infrared channels. The satellite data used in the present study correspond to night-time images in which large-scale stratiform clouds overlay the ocean. The procedure is based on the inversion of an atmospheric radiative transfer model that makes use of the discrete ordinates method called DISORT. A detailed study is presented which shows that cloud parameter retrieval is ambiguous, resulting in several possible solutions, because some pairs of effective particle radius and optical thickness produce the same brightness temperatures. The discretization of these two parameters, based on the cloud layer radiative behaviour, is proposed to avoid these multiple solutions. In addition, the model inversion presents some difficulties that have been solved using a genetic algorithm. The current retrieval scheme is applied to satellite data to compare results with local in situ measurements and a good agreement is obtained in those pixels near the sample site.

Retrieval of Marine Stratus Cloud Droplet Size from NOAA-AVHRR Nighttime Imagery

Abstract A method for retrieval of the droplet radius and temperature of oceanic stratocumulus is presented. It is based on night imagery obtained from the infrared channels of NOAA–AVHRR and an atmospheric radiative transfer model that makes use of the discrete ordinate method DISORT. It uses the observed satellite brightness temperature differences (BTD) between channels 4 and 5 to obtain the cloud temperature and between channels 3 and 4 to extract the effective radius of the cloud droplets. We also studied the peculiarities of the method, taking into account the behavior of the single scattering parameters, deduced from Mie theory, with droplet size. Results obtained are compared with in situ data collected at the Canary Islands (Spain) during summer 1996.

E-Dihydrorhodophytin, A C15 acetogenin from the red alga Laurencia pinnatifida

Abstract From the red alga Laurencia pinnatifida we have isolated E and Z dihydrorhodophytin. The structure and absolute configuration of the former have been established by spectroscopical and chemical methods.

Using a Mesoscale Meteorological Model to Reduce the Effect of Tropospheric Water Vapour from DInSAR Data: A Case Study for the Island of Tenerife, Canary Islands

Measurements of ground displacement through classical Differential Interferometric SAR (DInSAR) and advanced DInSAR techniques have been carried out over the entire actively volcanic island of Tenerife, Canary Islands. However, a detailed analysis of the effect of tropospheric water vapour on DInSAR at Tenerife should be carried out to evaluate its influence, including correction models that might improve the accuracy of DInSAR derived deformation signals. Unlike water vapour correction models that are based on space platforms (e.g. MODIS and MERIS), we present an alternative approach that is based on precise water vapour estimations derived from mesoscale numerical meteorological models, in particular the Weather Research and Forecasting (WRF) model. The application of this approach to a set of DInSAR observations of the island of Tenerife shows encouraging results.

An object-oriented daytime land-fog-detection approach based on the mean-shift and full lambda-schedule algorithms using EOS/MODIS data

A new algorithm is presented for land-fog detection using daytime imagery from the Earth Observing System (EOS) Moderate Resolution Imaging Spectroradiometer (MODIS) data. MODIS images constitute an ideal data source for fog detection due to their outstanding spatial and spectral resolution. In this article, a parameter named the Normalized Difference Fog Index (NDFI) is proposed, based on analysing the spectral character of fog and cloud by utilizing the Streamer radiative-transfer model and MODIS data. A mean-shift segmentation method is used to preliminary segment the NDFI image, and a full lambda-schedule algorithm is then iteratively applied to merge adjacent segments based on the combination of spectral and spatial information. Then, some properties (e.g. mean value of brightness temperature) are calculated for each segment, and each object is identified as either fog or not. The algorithms performance is evaluated against ground-based measurements over China in winter, and the algorithm is proved to be effective in detecting fog accurately based on three cases.

13C NMR assignments of some dibenzyl-γ-butyrolactone lignans

Abstract 1 H 13 C heteronuclear correlation and long-range correlation spectra with inverse detection, allowed the unequivocal assignment of 13 C spectra of some dibenzyl-γ-butyrolactones isolated from Bupleurum salicifolium .

High-Resolution Future Projections of Temperature and Precipitation in the Canary Islands

AbstractThe complex orography of the Canary Islands favors the creation of microclimates, which cannot be studied using global climate models or regional models with moderate resolution. In this work, WRF is used to perform a dynamic climate regionalization in the archipelago, using the pseudo–global warming technique to compute the initial and boundary conditions from a reanalysis dataset and from results of 14 global climate models. The simulations were performed for three decades, one at present (1995–2004) and two in the future (2045–54 and 2090–99), and for two different greenhouse gas scenarios (RCP4.5 and RCP8.5), defined in phase 5 of the Coupled Model Intercomparison Project. The obtained results, at a 5-km horizontal resolution, show a clear dependence of temperature increase with height and a positive change in diurnal temperature range, which is mainly due to a reduction in soil moisture and a slight decrease in cloud cover. This negative change in soil moisture is mainly a consequence of a de...

A new diterpene from Aplysia dactylomela

The homeobox gene extradenticle (exd) acts as a cofactor of the homeotic genes in the specification of larval patterns during embryogenesis. To study its role in adult patterns, we have generated clones of mutant exd- cells and examined their effect on the different body parts. In some regions, exd- clones exhibit homeotic transformations similar to those produced by known homeotic mutations such as Ultrabithorax (Ubx), labial (lab), spineless-aristapedia (ssa) or Antennapedia (Antp). In other regions, the lack of exd causes novel homeotic transformations producing ectopic eyes and legs. Moreover, exd is also required for functions normally not associated with homeosis, such as the maintenance of the dorsoventral pattern, the specification of subpatterns in adult appendages or the arrangement of bristles in the mesonotum and genitalia. Our findings indicate that exd is critically involved in adult morphogenesis, not only in the homeotic function but also in several other developmental processes.Previously published experiments have shown that the endogenous Dfd gene can be ectopically activated by its own (heat-shock-driven) product in a subset of cells of different segments. This results in the differentiation of maxillary structures like cirri and mouth hooks in places where they normally do not appear, and represents a phenomenon of autocatalysis of homeotic gene function that differs from the normal activation process. We show that this out-of-context activation occurs in cells belonging to the anterior compartments of the three thoracic and the A1 to A8 abdominal segments and that it requires the normal function of the polarity genes wingless (wg) and engrailed (en). The wg product, in addition to that of Dfd, appears to be sufficient to activate the endogenous Dfd gene in many embryonic cells. We have studied the effect of several homeotic genes on Dfd activation and phenotypic expression: Scr, Antp, Ubx and Abd-B repress Dfd both transcriptionally and at the phenotypic level, if their products are in sufficient amounts. The endogenous abd-A gene does not have a noticeable effect, but when it is replaced by an hsp70-abd-A gene, which produces a high and uniform level of expression, the phenotypic expression of Dfd is suppressed. Our results also suggest that the differentiation of cirri is induced by Dfd-expressing cells in non-expressing neighboring cells, and that this interaction occurs across the parasegmental border.During evolution, many animal groups have developed specialised outgrowths of the body wall, limbs or appendages. The type of appendage depends on the identity of the segment where they appear, indicating that the Hox genes contribute to appendage specification. Moreover, work carried out principally in Drosophila has identified the gene products and the mechanisms involved in pattern formation in the appendages. In this essay, we compare the morphogenetic processes in the appendages and the body wall; the function of the Hox genes and the response to the signalling molecules involved in local patterning. We speculate that, although the basic mechanisms are similar, there are significant differences in the manner the body trunk and appendages respond to them.[ES] La pared celular es un elemento morfogenetico esencial que determina la forma final de las celulas y que las protege contra la lisis. En S. pombe esta esta constituida por ? y s-glucano y manoproteinas y tanto la sintesis como remodelacion de su estructura requiere de diferentes enzimas estrictamente reguladas. En S. pombe existe poca informacion de como se lleva a cabo la incorporacion del material de membrana y sobre la regulacion de las enzimas implicadas en la sintesis y remodelacion de la pared celular por los mecanismos de transporte vesicular. Para abordar el estudio de como el trafico vesicular mediado por clatrina afecta a la morfogenesis de S. pombe y en particular cual es su papel en la regulacion de la sintesis de la pared celular se ha analizado el papel tanto de la clatrina, mediante el analisis de diferentes mutantes de la cadena ligera de la clatrina, como el del adaptador AP-2, que interviene en el proceso de endocitosis mediada por clatrina. Se ha demostrado que la delecion de la cadena ligera de la clatrina resulta letal para las celulas de S. pombe y que esta letalidad se rescata al incubar las celulas en un medio suplementado con sorbitol. En este caso aunque las celulas pueden sobrevivir poseen graves defectos morfologicos, en crecimiento, en trafico vesicular, en desarrollo sexual, etc. Se ha podido comprobar que la ausencia de Clc1p afecta drasticamente a la estabilidad de Chc1p hecho que hace que, a diferencia de otros organismos, la supervivencia de S. pombe sea mas dependiente de la presencia clatrina. Ademas se ha demostrado que la letalidad causada por la ausencia de Clc1p se debe principalmente a defectos graves en la sintesis de la pared celular que afectan directamente a la sintesis del glucano. Los resultados obtenidos muestran que una reduccion en la cantidad de clatrina causa un leve impacto en el transporte vesicular en general y en otros procesos y elementos biologicos, pero afecta gravemente a la secrecion de enzimas de sintesis/remodelacion de la pared celular, como las s(1,3)glucan sintasa y endoglucanasas. En cuanto al complejo adaptador AP-2 se ha comprobado, que a diferencia de lo que se conoce hasta el momento en otros organismos unicelulares, este forma un complejo con la clatrina y se ha demostrado que tiene un papel en la endocitosis general de S. pombe. Asi mismo se ha descubierto que AP-2 puede estar interviniendo en la sintesis de la pared celular ya que su ausencia afecta a la actividad s-glucan sintasa y hace que S. pombe sea hiper-sensible a compuestos que afectan a la integridad de la pared celular.We characterized a novel protein of the Ras family, p19 (H-RasIDX). The c-H-ras proto-oncogene undergoes alternative splicing of the exon termed IDX. We show that the alternative p19 mRNA is stable and as abundant as p21 (p21 H-Ras4A) mRNA in all of the human tissues and cell lines tested. IDX is spliced into stable mRNA in different mammalian species, which present a high degree of nucleotide conservation. Both the endogenous and the transiently expressed p19 protein are detected in COS-1 and HeLa cells and show nuclear diffuse and speckled patterns as well as cytoplasmic localization. In yeast two-hybrid assays, p19 did not interact with two known p21 effectors, Raf1 and Rin1, but was shown to interact with RACK1, a scaffolding protein that promotes multiprotein complexes in different signaling pathways. This observation suggests that p19 and p21 play differential and complementary roles in the cell.Resumen del trabajo presentado al Congreso Nacional de Biotecnologia, celebrado en Murcia del 18 al 21 de junio de 2017.A. G. G. thanks Ramon Areces Foundation for a grant. J. C. thanks NIH-CA24487 for financial support.Ministerio de Educacion y Ciencia and grant S-0505/MAT-0283 from Comunidad Autonoma de Madrid to M.S. and by an Institutional grant from Fundacion Ramon Areces to the Centro de Biologia Molecular “Severo Ochoa”We report a genetic and molecular study of UbxMX6 and Ubx195rx1, two mutations in the Ultrabithorax (Ubx) locus which appear to have a strong effect on the activity of the homologous Ubx gene. These mutations show the characteristic embryonic and adult phenotypes of Ubx null alleles, and also fail to produce any detectable Ubx product. Yet, genetic and phenotypic analyses involving a large number of trans heterozygous combinations of UbxMX6 and Ubx195rx1 with different classes of Ubx mutations, indicate that they hyperactivate the homologous gene. This effect is induced on wildtype or mutant forms of Ubx, provided that the pairing in the bithorax region is normal, i.e. these mutations have a strong positive effect on transvection. We also show that, unlike all the other known cases of transvection in Ubx, this is not zeste-dependent. Southern analyses indicate that UbxMX6 is a 3.4 kb deletion, and Ubx195rx1 is an approximately 11 kb insertion of foreign DNA, both in the promoter region. We speculate that the region altered in the mutations may have a wildtype function to ensure cis-autonomy of the regulation of Ubx transcription.Resumen del trabajo presentado al Congreso Nacional de Biotecnologia, celebrado en Murcia del 18 al 21 de junio de 2017.The pannier (pnr) gene of Drosophila encodes a zinc-finger transcription factor of the GATA family and is involved in several developmental processes during embryonic and imaginal development. We report some novel aspects of the regulation and function of pnr during embryogenesis. Previous work has shown that pnr is activated by decapentaplegic (dpp) in early development, but we find that after stage 10, the roles are reversed and pnr becomes an upstream regulator of dpp. This function of pnr is necessary for the activation of the Dpp pathway in the epidermal cells implicated in dorsal closure and is not mediated by the JNK pathway, which is also necessary for Dpp activity in these cells. In addition, we show that pnr behaves as a selector-like gene in generating morphological diversity in the dorsoventral body axis. It is responsible for maintaining a subdivision of the dorsal half of the embryo into two distinct, dorsomedial and dorsolateral, regions, and also specifies the identity of the dorsomedial region. These results, together with prior work on its function in adults, suggest that pnr is a major factor in the genetic subdivision of the body of Drosophila.10th International Symposium on Reproductive Physiology of Fish (10th ISRPF), Expanding the khowledge base of reproductive success: from genes to the environment, 25-30 May 2014, Olhao, Portugal.-- 1 pageBy using a hsp70-Ubx fusion gene, we have ectopically expressed a Ubx product in the embryonic head primordia and studied the developmental effects on the larval head. We find that after high and persistent levels of Ubx product, the head is replaced by three (C1, C2 and C3) abdominal-like denticle belts. The C2 and C3 belts are the homeotic transformations of parasegments 1 and 2, respectively, while the C1 belt probably derives from the transformation and subsequent fusion of the most anterior procephalic primordia. On the basis of their response to the Ubx product and other arguments, we propose that the larval head is made of two genetically distinct components; one is the procephalon and the anterior region of the mandibular lobe, and the other is part of the parasegmental trunk and includes parasegments 1 and 2. Our results also indicate that most or all the larval head structures derive from precursor cells of ventral origin.The Iroquois (Iro) family of genes are found in nematodes, insects and vertebrates. They usually occur in one or two genomic clusters of three genes each and encode transcriptional controllers that possess a characteristic homeodomain. The Iro genes function early in development to specify the identity of diverse territories of the body, such as the dorsal head and dorsal mesothorax of Drosophila and the neural plate of Xenopus. In some aspects they act in the same way as classical selector genes, but they display specific properties that place them into a category of their own. Later in development in both Drosophila and vertebrates, the Iro genes function again to subdivide those territories into smaller domains.The pannier (pnr) gene encodes a GATA transcription factor and acts in several developmental processes in Drosophila, including embryonic dorsal closure, specification of cardiac cells and bristle determination. We show that pnr is expressed in the mediodorsal parts of thoracic and abdominal segments of embryos, larvae and adult flies. Its activity confers cells with specific adhesion properties that make them immiscible with non-expressing cells. Thus there are two genetic domains in the dorsal region of each segment: a medial (MED) region where pnr is expressed and a lateral (LAT) region where it is not. The homeobox gene iroquois (iro) is expressed in the LAT region. These regions are not formed by separate polyclones of cells, but are defined topographically. We show that ectopic pnr in the wing induces MED thoracic development, indicating that pnr specifies the identity of the MED regions. Correspondingly, when pnr is removed from clones of cells in the MED domain, they sort out and apparently adopt the LAT fate. We propose that (1) the subdivision into MED and LAT regions is a general feature of the Drosophila body plan and (2) pnr is the principal gene responsible for this subdivision. We argue that pnr acts like a classical selector gene but differs in that its expression is not propagated through cell divisions.We have developed a specific polyclonal antibody that recognizes the protein products of the abdominal-A (abd-A) gene, a member of the bithorax complex of Drosophila. The normal expression domain extends from parasegments 7 to 13, in good correspondence with previous genetic and molecular results. However, while the anterior border of expression is precisely demarcated by a parasegmental boundary, the posterior border does not coincide with a lineage boundary. Within the normal domain, the expression of abd-A shows intrametameric modulation; the amount of product is higher in posterior compartments and in the most anterior cells of the anterior compartments and then gradually decreases. We have examined the effect on abd-A expression of a number of mutations, some mapping within and others outside the abd-A transcription unit. Those mapping to the transcription unit eliminate or severely reduce the amount of abd-A antigen, while those mapping outside produce an abnormal distribution of abd-A protein. Finally, we show that the abd-A gene is down-regulated in part of the Abdominal-B (Abd-B) domain, precisely in those regions where the Abd-B gene is expressed at high levels.Resumen del trabajo presentado al Yeast Genetics Meeting, celebrado en Stanford, California (USA) del 22 al 26 de agosto de 2018.The effect of the anti-tumoral drug lauryl gallate on the infectivity of the African swine fever virus among other DNA (Herpes simplex and Vaccinia) and RNA (Influenza, Porcine transmissible gastroenteritis and Sindbis) viruses, involved in animal and human diseases, is analyzed. Viral production was strongly inhibited in different cell lines at non-toxic concentrations of the drug (1-10 μM), reducing the titres from 3 to more than 5 log. units depending on the multiplicity of infection. In our model system (African swine fever virus in Vero cells), the addition of the drug 1 h before virus adsorption, completely abolished virus productivity in a one-step growth virus cycle. Interestingly, no inhibitory effect was observed when lauryl gallate was added after 5 to 8 hpi. Both cellular and viral DNA synthesis and late viral transcription were inhibited by the drug, but, however, the early viral protein synthesis and the virus-mediated increasing of p53 remained unaffected. Activation of the apoptotic effector caspase-3 was not detected after lauryl gallate treatment of Vero cells, and, furthermore, the presence of the drug abrogated the activation of this protease induced by the virus infection. The overall results likely indicate that a cellular factor/function might be the target of the antiviral action of alkyl gallates.Tesis Doctoral presentada por Eduardo Rodenas Martinez en el Centro Andaluz de Biologia del Desarrollo, centro mixto CSIC-UPO.Resumen del trabajo presentado al Yeast Genetics Meeting, celebrado en Stanford, California (USA) del 22 al 26 de agosto de 2018.