Albenones José de Mesquita

EFICIÊNCIA DOS CALDOS DE ENRIQUECIMENTO (LEB1 E LEB2) E DOS ÁGARES SELETIVOS (LPM E LSAB-CAN) NO ISOLAMENTO DE BACTÉRIAS DO GÊNERO Listeria EM CARNE BOVINA E ÁGUA RESIDUÁRIA DE LAVAGEM DE CARCAÇA

Rosa pinna ( Clerodendrum philippinum Schaue r), is a shrub with attractive and fragrant flowers. However, the tall and spread architecture and high leaf to flower ratio hinder its use as an ornamental plant. Hence, changing the level of acceptance of C. philippinum by incorporating a dwarfing gene to the plant is considered useful. A study was conducted to find a suitable regeneration medium and ultimately to modify the plant architecture through transformation of the gibberellic acid inhibitor (gai) mutant dwarfing gene by particle bombardment. Effects of different concentrations of thidiazuron (TDZ:Nphenyl N’ 1,2,3-thidiazol-5-yl urea) on callus initiation and regeneration of shoots and the effects of micro career flying distance in gene transformation of C. philippinum were investigated. The regenerated shoots were first cultured on hormone free MS medium and transferred to a MS medium with 0.5 mg/L in dole butyric acid for rooting after four weeks. The shortest duration for callus initiation, the largest callus volume (28 days after establishment) and the highest number of shoots (75 days after establishment) were observed in 1.5 mg/L TDZ in the MS medium. Results of transferring the gai mutant gene to in vitro leaves of C. philippinum revealed that both the shortest time duration and the highest plant regeneration were at 9 cm micro-carrier flying distance. Leaf tissues of regenerated plants, which carry the gai mutant gene, positively responded for the beta-glucuronidase (GUS) assay. The regenerated shoots were rooted and successfully acclimatized. Tropical Agricultural Research Vol. 25 (1): 27-37 (2013)

Utilização de Silicea 12CH e Bioterápico Complexo 30CH na Contagem de Células Somáticas em Vacas Leiteiras

Esta pesquisa foi realizada com o objetivo de avaliar o efeito da utilizacao de Silicea 12CH e do Bioterapico complexo 30 CH (de cepas oriundas do leite) no tratamento da mastite subclinica. Foram utilizadas 26 vacas mesticas leiteiras, com mastite subclinica diagnosticada pela contagem de celulas somaticas (CCS). Os animais foram sorteados aleatoriamente em dois grupos: controle e tratamento. O bioterapico complexo foi elaborado a partir dos microrganismos isolados das amostras de leite proveniente dos dois grupos do experimento. O Grupo tratamento recebeu 15 gotas de Silicea 12CH e 15 gotas do Bioterapico complexo 30CH, adicionados em 50 gramas de acucar cristal. O grupo controle recebeu 30 gotas de solucao hidroalcoolica a 70%, tambem adicionado a 50 gramas de acucar cristal. Os medicamentos e o placebo foram administrados individualmente a cada animal, por ocasiao das ordenhas diarias, durante 90 dias. Realizou-se quinzenalmente a CCS de todos os animais. Nao se observou diferenca significativa (P > 0,05) entre a CCS do grupo experimental e controle. A Silicea 12CH e o bioterapico complexo 30CH nao foram capazes de provocar reducao da CCS do grupo experimental.