Albert Guisasola

Roof selection for rainwater harvesting: Quantity and quality assessments in Spain

Roofs are the first candidates for rainwater harvesting in urban areas. This research integrates quantitative and qualitative data of rooftop stormwater runoff in an urban Mediterranean-weather environment. The objective of this paper is to provide criteria for the roof selection in order to maximise the availability and quality of rainwater. Four roofs have been selected and monitored over a period of 2 years (2008-2010): three sloping roofs - clay tiles, metal sheet and polycarbonate plastic - and one flat gravel roof. The authors offer a model for the estimation of the runoff volume and the initial abstraction of each roof, and assess the physicochemical contamination of roof runoff. Great differences in the runoff coefficient (RC) are observed, depending mostly on the slope and the roughness of the roof. Thus, sloping smooth roofs (RC>0.90) may harvest up to about 50% more rainwater than flat rough roofs (RC=0.62). Physicochemical runoff quality appears to be generally better than the average quality found in the literature review (conductivity: 85.0 ± 10.0 μS/cm, total suspended solids: 5.98 ± 0.95 mg/L, total organic carbon: 11.6 ± 1.7 mg/L, pH: 7.59 ± 0.07 upH). However, statistically significant differences are found between sloping and flat rough roofs for some parameters (conductivity, total organic carbon, total carbonates system and ammonium), with the former presenting better quality in all parameters (except for ammonium). The results have an important significance for local governments and urban planners in the (re)design of buildings and cities from the perspective of sustainable rainwater management. The inclusion of criteria related to the roofs slope and roughness in city planning may be useful to promote rainwater as an alternative water supply while preventing flooding and water scarcity.

Sulfur transformation in rising main sewers receiving nitrate dosage

The anoxic and anaerobic sulfur transformation pathways in a laboratory-scale sewer receiving nitrate were investigated. Four reactors in series were employed to imitate a rising main sewer. The nitrate-dosing strategy was effective in controlling sulfide, as confirmed by the long-term sulfide measurements. Anoxic sulfide oxidation occurred in two sequential steps, namely the oxidation of sulfide to elemental sulfur (S(0)) and the oxidation of S(0) to sulfate (SO(4)(2-)). The second oxidation step, which primarily occurred when the first step was completed, had a rate that is approximately 15% of the first step. When nitrate was depleted, sulfate and elemental sulfur were reduced simultaneously to sulfide. Sulfate reduction had a substantially higher rate (5 times) than S(0) reduction. The relatively slower S(0) oxidation and reduction rates implied that S(0) was an important intermediate during anoxic and anaerobic sulfur transformation. Electron microscopic studies indicated the presence of elemental sulfur, which was at a significant level of 9.9 and 16.7 mg-S/g-biomass in nitrate-free and nitrate-exposed sewer biofilms, respectively. A conceptual sulfur transformation model was established to characterize predominant sulfur transformations in rising main sewers receiving nitrate dosage. The findings are pertinent for optimizing nitrate dosing to control sulfide in rising main sewers.

Development of a model for assessing methane formation in rising main sewers

Significant methane formation in sewers has been reported recently, which may contribute significantly to the overall greenhouse gas emission from wastewater systems. The understanding of the biological conversions occurring in sewers, particularly the competition between methanogenic and sulfate-reducing populations for electron donors, is an essential step for minimising methane emissions from sewers. This work proposes an extension to the current state-of-the-art models characterising biological and physicochemical processes in sewers. This extended model includes the competitive interactions of sulfate-reducing bacteria and methanogenic archaea in sewers for various substrates available. The most relevant parameters of the model were calibrated with lab-scale experimental data. The calibrated model described field data reasonably well. The model was then used to investigate the effect of several key sewer design and operational parameters on methane formation. The simulation results showed that methane production was highly correlated with the hydraulic residence time (HRT) and pipe area to volume (A/V) ratio showing higher methane concentrations at a long HRT or a larger A/V ratio.

The nature of the carbon source rules the competition between PAO and denitrifiers in systems for simultaneous biological nitrogen and phosphorus removal

The presence of nitrate in the theoretical anaerobic reactor of a municipal WWTP aiming at simultaneous C, N and P removal usually leads to Enhanced Biological Phosphorus Removal (EBPR) failure due to the competition between PAO and denitrifiers for organic substrate. This problem was studied in a continuous anaerobic-anoxic-aerobic (A2/O) pilot plant (146 L) operating with good removal performance and a PAO-enriched sludge (72%). Nitrate presence in the initially anaerobic reactor was studied by switching the operation of the plant to an anoxic-aerobic configuration. When the influent COD composition was a mixture of different carbon sources (acetic acid, propionic acid and sucrose) the system was surprisingly able to maintain EBPR, even with internal recycle ratios up to ten times the influent flow rate and COD limiting conditions. However, the utilisation of sucrose as sole carbon source resulted in a fast EBPR failure. Batch tests with different nitrate concentrations (0-40 mg L(-1)) were performed in order to gain insight into the competition for the carbon source in terms of P-release or denitrification rates and P-release/C-uptake ratio. Surprisingly, no inhibitory or detrimental effect on EBPR performance due to nitrate was observed. A model based on ASM2d but considering two step nitrification and denitrification was developed and experimentally validated. Simulation studies showed that anaerobic VFA availability is critical to maintain EBPR activity.

Impact of nitrate addition on biofilm properties and activities in rising main sewers

Anaerobic sewer biofilm is a composite of many different microbial populations, including sulfate reducing bacteria (SRB), methanogens and heterotrophic bacteria. Nitrate addition to sewers in an attempt to control hydrogen sulfide concentrations affects the behaviour of these populations, which in turn impacts on wastewater characteristics. Experiments were carried out on a laboratory reactor system simulating a rising main to determine the impact of nitrate addition on the microbial activities of anaerobic sewer biofilm. Nitrate was added to the start of the rising main during sewage pump cycles at a concentration of 30 mg-N L(-1) for over 5 months. While it reduced sulfide levels at the outlet of the system by 66%, nitrate was not toxic or inhibitory to SRB activity and did not affect the dominant SRB populations in the biofilm. Long-term nitrate addition in fact stimulated additional SRB activity in downstream biofilm. Nitrate addition also stimulated the activity of nitrate reducing, sulfide oxidizing bacteria that appeared to be primarily responsible for the prevention of sulfide build up in the wastewater in the presence of nitrate. A short adaptation period of three to four nitrate exposure events (approximately 10 h) was required to stimulate biological sulfide oxidation, beyond which no sulfide accumulation was observed under anoxic conditions. Nitrate addition effectively controlled methane concentrations in the wastewater. The nitrate uptake rate of the biofilm increased with repeated exposure to nitrate, which in turn increased the consumption of biodegradable COD in the wastewater. These results provide a comprehensive understanding of the impact of nitrate addition on wastewater composition and sewer biofilm microbial activities, which will facilitate optimization of nitrate dosing for effective sulfide control in rising main sewers.

Improving the performance of a WWTP control system by model-based setpoint optimisation

The aim of this work was the improvement of a WWTP control system using a model-based setpoint optimisation. For this purpose, an anaerobic/anoxic/aerobic (A2/O) pilot WWTP was simulated using the IWA ASM2d model under different influent conditions. Several control strategies for an efficient biological C/N/P removal were evaluated in this WWTP: i) open loop; ii) dissolved oxygen control in the aerated reactors; iii) maximum performance of nutrient removal; iv) optimised fixed setpoints for the controlled variables; v) daily optimised setpoints; vi) two different sets of optimised setpoints for weekdays and weekends and vii) hourly optimised setpoints. A single cost function based on the operating costs by converting the effluent quality into monetary units was chosen for evaluating the plant performance (i.e. the control loops setpoints were optimised to obtain low effluent N and P discharges with the minimum costs). Setpoint optimisation was shown as a good tool to improve the performance of the system. In this case study, control strategy (vi) was selected as the best choice considering the trade-off cost-benefit. The optimised control system resulted in around a 45% decrease of operational costs with respect to the open loop scenario, a significant improvement of the effluent quality and a drastic decrease of the time above discharge limits.

A review on nitrous oxide (N2O) emissions during biological nutrient removal from municipal wastewater and sludge reject water

Nitrous oxide (N2O) is an important pollutant which is emitted during the biological nutrient removal (BNR) processes of wastewater treatment. Since it has a greenhouse effect which is 265 times higher than carbon dioxide, even relatively small amounts can result in a significant carbon footprint. Biological nitrogen (N) removal conventionally occurs with nitrification/denitrification, yet also through advanced processes such as nitritation/denitritation and completely autotrophic N-removal. The microbial pathways leading to the N2O emission include hydroxylamine oxidation and nitrifier denitrification, both activated by ammonia oxidizing bacteria, and heterotrophic denitrification. In this work, a critical review of the existing literature on N2O emissions during BNR is presented focusing on the most contributing parameters. Various factors increasing the N2O emissions either per se or combined are identified: low dissolved oxygen, high nitrite accumulation, low chemical oxygen demand to nitrogen ratio, slow growth of denitrifying bacteria, uncontrolled pH and temperature. However, there is no common pattern in reporting the N2O generation amongst the cited studies, a fact that complicates its evaluation. When simulating N2O emissions, all microbial pathways along with the potential contribution of abiotic N2O production during wastewater treatment at different dissolved oxygen/nitrite levels should be considered. The undeniable validation of the robustness of such models calls for reliable quantification techniques which simultaneously describe dissolved and gaseous N2O dynamics. Thus, the choice of the N-removal process, the optimal selection of operational parameters and the establishment of validated dynamic models combining multiple N2O pathways are essential for studying the emissions mitigation.

Glycerol as a sole carbon source for enhanced biological phosphorus removal

Wastewaters with low organic matter content are one of the major causes of EBPR failures in full-scale WWTP. This carbon source deficit can be solved by external carbon addition and glycerol is a perfect candidate since it is nowadays obtained in excess from biodiesel production. This work shows for the first time that glycerol-driven EBPR with a single-sludge SBR configuration is feasible (i.e. anaerobic glycerol degradation linked to P release and aerobic P uptake). Two different strategies were studied: direct replacement of the usual carbon source for glycerol and a two-step consortium development with glycerol anaerobic degraders and PAO. The first strategy provided the best results. The implementation of glycerol as external carbon source in full-scale WWTP would require a suitable anaerobic hydraulic retention time. An example using dairy wastewater with a low COD/P ratio confirms the feasibility of using glycerol as an external carbon source to increase P removal activity. The approach used in this work opens a new range of possibilities and, similarly, other fermentable substrates can be used as electron donors for EBPR.

Enhanced Performance of Bioelectrochemical Hydrogen Production using a pH Control Strategy

The use of membranes in microbial electrolysis cells (MEC) is required to obtain high-purity hydrogen and to avoid the consumption of hydrogen by undesired microorganisms. However, its utilization results in pH gradients across the membrane that contribute to potential losses and reduce the efficiency of MEC. Several pH-controlled and noncontrolled scenarios were evaluated in this work, which evidenced that pH control is beneficial for the MEC performance. The best results were obtained if the anodic and cathodic pH were controlled at 7.5 and 2.0, respectively, to produce 0.58 m(3)  m(-3)  d(-1) of hydrogen at an applied voltage of only 0.2 V. The energy efficiency with respect to the electrical input was increased up to 883 %. Anodic pH control allowed us to maintain a stable exoelectrogenic activity with practically constant current intensity, whereas cathodic pH control at 2.0 allowed a fivefold decrease of the required electrical input, which opens new opportunities for the economy of its full-scale application.

Microbial community analysis in a long-term membrane-less microbial electrolysis cell with hydrogen and methane production

A single-chamber microbial electrolysis cell (MEC) aiming at hydrogen production with acetate as sole carbon source failed due to methanogenesis build-up despite the significant amount of 2-bromoethanesulfonate (BES) dosage, 50 mM. Specific batch experiments and a thorough microbial community analysis, pyrosequencing and qPCR, of cathode, anode and medium were performed to understand these observations. The experimental data rebuts different hypothesis and shows that methanogenesis at high BES concentration was likely due to the capacity of some Archaea (hydrogen-oxidizing genus Methanobrevibacter) to resist high BES concentration up to 200 mM. Methanobrevibacter, of the Methanobacteriales order, represented almost the 98% of the total Archaea in the cathode whereas Geobacter was highly abundant in the anode (72% of bacteria). Moreover, at higher BES concentration (up to 200 mM), methanogenesis activity decreased resulting in an increase of homoacetogenic activity, which challenged the performance of the MEC for H2 production.