Albert U. Tenuta

Integrated management strategies for Phytophthora sojae combining host resistance and seed treatments

Phytophthora sojae has re-emerged as a serious soybean pathogen in the past decade. This may be due in part to changes in resistance levels in current cultivars, adoption of P. sojae populations to deployed Rps genes, and highly favorable environments in the past decade. This multilocation study evaluated the effect of seed treatments on the incidence and severity of Phytophthora root and stem rot on soybeans with different combinations of Rps genes and levels of partial resistance. The efficacy of the seed treatments was highly variable across locations. Seed treatments (metalaxyl and mefenoxam) provided protection and increased yields across cultivars in locations where rain or irrigation occurred shortly after planting (Ohio, South Dakota, and Ontario). However, there were no significant differences in stand or yield consistently across cultivars in Iowa, Nebraska, Wisconsin, or Ohio, where heavy precipitation did not occur until later growth stages. The environment, levels of inoculum, and pathogen complex may have played a role in the different responses to the seed treatments and to the different combinations of Rps genes and levels of partial resistance to P. sojae in the cultivars. Fields that are poorly drained and have P. sojae populations with complex pathotypes may benefit the most from seed treatments. Individual fields where producers may see the greatest benefit to utilizing these integrated management strategies will need to be identified.

Phytophthora root and stem rot of soybean.

Phytophthora sojae is a soil borne pathogen that in the past has caused very large economic losses. During the late 1970s, 300,000 soybean acres (approximately 10% of total soybean production in Ohio) were lost due to P. sojae. This disease has since been effectively managed predominately through the incorporation of singlegene mediated resistance but quantitative or partial resistance has been used as well. In fact, today, we can repeat 100% loss by planting soybean cultivars that were popular during earlier epidemics. Without high levels of resistance to this pathogen, many soybean acres would be lost each year to this disease. Phytophthora doesn’t forget and it doesn’t go away! Disciplines Agricultural Science | Agriculture | Plant Pathology Comments This is an article from The Plant Health Instructor (2007): 1, doi:10.1094/PHI-I-2007-0830-07. Posted with permission. Rights Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted. Authors Anne E. Dorrance, Dennis Mills, Alison E. Robertson, Martin A. Draper, Loren Giesler, and Albert Tenuta This article is available at Iowa State University Digital Repository: http://lib.dr.iastate.edu/plantpath_pubs/86 Phytophthora root and stem rot of soybean www.apsnet.org /edcenter/intropp/lessons/fungi/Oomycetes/Pages/PhytophthoraSojae.aspx Dorrance, A.E., D. Mills, A.E. Robertson, M.A. Draper, L. Giesler, and A.Tenuta, 2007. Phytophthora root and stem rot of soybean. The Plant Health Instructor. DOI: 10.1094/PHI-I-2007-0830-07 Reviewed 2012. DISEASE: Phytophthora root and stem rot of soybean PATHOGEN: Phytophthora sojae HOSTS: Soybean: Glycine max, the cultivated soybean, is the primary economic host. Glycine soja, the wild progenitor of soybean, is also susceptible Authors Anne E. Dorrance and Dennis Mills, The Ohio State University Alison E. Robertson, Iowa State University Martin A. Draper, USDA-CSREES, formerly @ South Dakota State University Loren Giesler, University of Nebraska-Lincoln Albert Tenuta, Ontario Ministry of Agriculture, Canada

Corn yield loss estimates due to diseases in the United States and Ontario, Canada from 2012 to 2015.

Annual decreases in corn yield caused by diseases were estimated by surveying members of the Corn Disease Working Group in 22 corn-producing states in the United States and in Ontario, Canada, from 2012 through 2015. Estimated loss from each disease varied greatly by state and year. In general, foliar diseases such as northern corn leaf blight, gray leaf spot, and Goss’s wilt commonly caused the largest estimated yield loss in the northern United States and Ontario during nondrought years. Fusarium stalk rot and plant-parasitic nematodes caused the most estimated loss in the southernmost United States. The estimated mean economic loss due to yield loss by corn diseases in the United States and Ontario from 2012 to 2015 was

A Coordinated Effort to Manage Soybean Rust in North America: A Success Story in Soybean Disease Monitoring

76.51 USD per acre. The cost of disease-mitigating strategies is another potential source of profit loss. Results from this survey will provide scientists, breeders, government, and educators with data to help inform and prioritize research, policy, and educational efforts in corn pathology and disease management. M U E L L E R E T A L . , P L A N T H E A L T H P R O G R E S S 1 7 (2 0 1 6 )

Oomycete Species Associated with Soybean Seedlings in North America—Part I: Identification and Pathogenicity Characterization

Existing crop monitoring programs determine the incidence and distribution of plant diseases and pathogens and assess the damage caused within a crop production region. These programs have traditionally used observed or predicted disease and pathogen data and environmental information to prescribe management practices that minimize crop loss. Monitoring programs are especially important for crops with broad geographic distribution or for diseases that can cause rapid and great economic losses. Successful monitoring programs have been developed for several plant diseases, including downy mildew of cucurbits, Fusarium head blight of wheat, potato late blight, and rusts of cereal crops. A recent example of a successful disease-monitoring program for an economically important crop is the soybean rust (SBR) monitoring effort within North America. SBR, caused by the fungus Phakopsora pachyrhizi, was first identified in the continental United States in November 2004. SBR causes moderate to severe yield losses globally. The fungus produces foliar lesions on soybean (Glycine max) and other legume hosts. P. pachyrhizi diverts nutrients from the host to its own growth and reproduction. The lesions also reduce photosynthetic area. Uredinia rupture the host epidermis and diminish stomatal regulation of transpiration to cause tissue desiccation and premature defoliation. Severe soybean yield losses can occur if plants defoliate during the mid-reproductive growth stages. The rapid response to the threat of SBR in North America resulted in an unprecedented amount of information dissemination and the development of a real-time, publicly available monitoring and prediction system known as the Soybean Rust-Pest Information Platform for Extension and Education (SBR-PIPE). The objectives of this article are (i) to highlight the successful response effort to SBR in North America, and (ii) to introduce researchers to the quantity and type of data generated by SBR-PIPE. Data from this system may now be used to answer questions about the biology, ecology, and epidemiology of an important pathogen and disease of soybean.

Fungicide and Cultivar Effects on Sudden Death Syndrome and Yield of Soybean

Oomycete pathogens are commonly associated with soybean root rot and have been estimated to reduce soybean yields in the United States by 1.5 million tons on an annual basis. Limited information exists regarding the frequency and diversity of oomycete species across the major soybean-producing regions in North America. A survey was conducted across 11 major soybean-producing states in the United States and the province of Ontario, Canada. In 2011, 2,378 oomycete cultures were isolated from soybean seedling roots on a semiselective medium (CMA-PARPB) and were identified by sequencing of the internal transcribed spacer region of rDNA. Sequence results distinguished a total of 51 Pythium spp., three Phytophthora spp., three Phytopythium spp., and one Aphanomyces sp. in 2011, with Pythium sylvaticum (16%) and P. oopapillum (13%) being the most prevalent. In 2012, the survey was repeated, but, due to drought conditions across the sampling area, fewer total isolates (n = 1,038) were collected. Additionally, in 2012, a second semiselective medium (V8-RPBH) was included, which increased the Phytophthora spp. isolated from 0.7 to 7% of the total isolates. In 2012, 54 Pythium spp., seven Phytophthora spp., six Phytopythium spp., and one Pythiogeton sp. were recovered, with P. sylvaticum (14%) and P. heterothallicum (12%) being recovered most frequently. Pathogenicity and virulence were evaluated with representative isolates of each of the 84 species on soybean cv. Sloan. A seed-rot assay identified 13 and 11 pathogenic species, respectively, at 13 and 20°C. A seedling-root assay conducted at 20°C identified 43 species as pathogenic, having a significantly detrimental effect on the seedling roots as compared with the noninoculated control. A total of 15 species were pathogenic in both the seed and seedling assays. This study provides a comprehensive characterization of oomycete species present in soybean seedling roots in the major production areas in the United States and Ontario, Canada and provides a basis for disease management and breeding programs.

Oomycete Species Associated with Soybean Seedlings in North America—Part II: Diversity and Ecology in Relation to Environmental and Edaphic Factors

The effect of fungicides on severity of sudden death syndrome (SDS; caused by Fusarium virguliforme), plant establishment, and soybean yield was evaluated in 12 field experiments conducted in Illinois, Indiana, Iowa, Michigan, and Ontario in 2013 and 2014. Two soybean cultivars that differed in susceptibility to SDS were planted in fields with a history of SDS or with artificial augmentation of F. virguliforme. Efficacy of seed, in-furrow, and foliar-applied fungicides was assessed. SDS levels varied across locations and years. Fluopyram applied on the seed or in-furrow reduced foliar disease index maximum up to 95% in 5 of the 12 experiments. In three experiments with significant (P < 0.10) treatment effect, fluopyram seed treatment improved yields up to 11% compared with the base seed treatment comprising prothioconazole + penflufen + metalaxyl and clothianidin + Bacillus firmus. Meta-analysis also indicated that the fluopyram seed treatment and in-furrow application were effective at reducing SDS and increasing yield relative to the control; however, the baseline disease influenced the yield and disease response to fungicide treatments. Treatment effect was not significant when disease pressure was low. The concentration of F. virguliforme DNA in soybean roots, measured by a specific real-time quantitative polymerase chain reaction assay, was not different among fungicide treatments in 9 of 10 experiments. Moderately resistant cultivars had less disease than susceptible cultivars, indicating that resistant cultivars in combination with fluopyram seed treatment or in-furrow application could provide effective management of SDS.

Effect of glyphosate application on sudden death syndrome of glyphosate-resistant soybean under field conditions

Soybean (Glycine max (L.) Merr.) is produced across a vast swath of North America, with the greatest concentration in the Midwest. Root rot diseases and damping-off are a major concern for production, and the primary causal agents include oomycetes and fungi. In this study, we focused on examination of oomycete species distribution in this soybean production system and how environmental and soil (edaphic) factors correlate with oomycete community composition at early plant growth stages. Using a culture-based approach, 3,418 oomycete isolates were collected from 11 major soybean-producing states and most were identified to genus and species using the internal transcribed spacer region of the ribosomal DNA. Pythium was the predominant genus isolated and investigated in this study. An ecology approach was taken to understand the diversity and distribution of oomycete species across geographical locations of soybean production. Metadata associated with field sample locations were collected using geographical information systems. Operational taxonomic units (OTU) were used in this study to investigate diversity by location, with OTU being defined as isolate sequences with 97% identity to one another. The mean number of OTU ranged from 2.5 to 14 per field at the state level. Most OTU in this study, classified as Pythium clades, were present in each field in every state; however, major differences were observed in the relative abundance of each clade, which resulted in clustering of states in close proximity. Because there was similar community composition (presence or absence) but differences in OTU abundance by state, the ordination analysis did not show strong patterns of aggregation. Incorporation of 37 environmental and edaphic factors using vector-fitting and Mantel tests identified 15 factors that correlate with the community composition in this survey. Further investigation using redundancy analysis identified latitude, longitude, precipitation, and temperature as factors that contribute to the variability observed in community composition. Soil parameters such as clay content and electrical conductivity also affected distribution of oomycete species. The present study suggests that oomycete species composition across geographical locations of soybean production is affected by a combination of environmental and edaphic conditions. This knowledge provides the basis to understand the ecology and distribution of oomycete species, especially those able to cause diseases in soybean, providing cues to develop management strategies.

Effect of Planting Date, Seed Treatment, and Cultivar on Plant Population, Sudden Death Syndrome, and Yield of Soybean

Sudden death syndrome (SDS), caused by Fusarium virguliforme, is an important yield limiting disease of soybean. Glyphosate is used to control weeds in soybean; however, its effect on SDS is not clearly understood. The objective of this study was to examine the impact of glyphosate on SDS, yield, and plant nutrition under field conditions. Fourteen field experiments were conducted in Iowa, Illinois, Indiana, Michigan, Wisconsin, and Ontario, Canada during 2011 to 2013. The experiment consisted of six treatment combinations of glyphosate and herbicides not containing glyphosate. Disease index was significantly different across the location-years, ranging from 0 to 65. The highest disease was noted in locations with irrigation, indicating that high soil moisture favors development of SDS. There were no effects of herbicide treatments or interactions on disease. The foliar disease index among the treatments over all years ranged from 9 to 13. Glyphosate-treatments also tended to yield more than treatments of herbicides not containing glyphosate. There were no interactions between glyphosate-treatments and total manganese in plant tissue. The interaction of glyphosate with other nutrients in plant tissue was inconclusive. This 14 location-year study demonstrated that glyphosate application did not increase SDS severity or adversely affect soybean yield under field conditions.

Benefits and profitability of fluopyram-amended seed treatments for suppressing sudden death syndrome and protecting soybean yield: A meta-analysis

A 2-year study was conducted in Illinois, Indiana, Iowa, and Ontario in 2013 and 2014 to determine the effects of planting date, seed treatment, and cultivar on plant population, sudden death syndrome (SDS) caused by Fusarium virguliforme, and grain yield of soybean (Glycine max). Soybean crops were planted from late April to mid-June at approximately 15-day intervals, for a total of three to four plantings per experiment. For each planting date, two cultivars differing in SDS susceptibility were planted with and without fluopyram seed treatment. Mid-May plantings resulted in higher disease index compared with other planting dates in two experiments, early June plantings in three, and the remaining six experiments were not affected by planting date. Soil temperature at planting was not linked to SDS development. Root rot was greater in May plantings for most experiments. Resistant cultivars had significantly lower disease index than the susceptible cultivar in 54.5% of the experiments. Fluopyram reduced disease severity and protected against yield reductions caused by SDS in nearly all plantings and cultivars, with a maximum yield response of 1,142 kg/ha. Plant population was reduced by fluopyram seed treatment and early plantings in some experiments; however, grain yield was not affected by these reductions. Yields of plots planted in mid-June were up to 29.8% less than yields of plots planted in early May. The lack of correlation between early planting date and SDS severity observed in this study indicates that farmers do not have to delay planting in the Midwest to prevent yield loss due to SDS; cultivar selection combined with fluopyram seed treatment can reduce SDS in early-planted soybean (late April to mid May).