Albert V. Hennessy

Isolation in Tissue Culture of an Interfering Agent from Patients with Rubella.

Summary The technic first described by Buescher and Parkman for isolation of an agent from patients with rubella was used in a study of 7 university students affected with this disease. 1. Agents which interfered with ECHO 11 in primary or continuous grivet monkey kidney cells or in a continuous rhesus monkey kidney cell line (LLC-MK2) were recovered from all patients. 2. The agent was recovered from upper respiratory secretions of all cases and from blood drawn during the acute phase in 4 of 7 patients. 3. Rise in the neutralizing properties of the sera obtained in the convalescent period as compared with the acute sera was a constant feature in all samples tested. 4. The usefulness of the LLC-MK2 cell line for primary isolation of this agent(s) was proven during this study.

The clinical epidemiology of Asian influenza.

Excerpt In February, 1957, viruses belonging to a previously unrecognized family of influenza A strains emerged from North China and within four months were disseminated throughout the globe.1, 2Th...

Studies on Vaccination of Infants Against Influenza with Influenza Hemagglutinin

Summary Infants 2-6 mo of age were given 3 doses of influenza hemagglutinin vaccine. One group received 250 CCA units of vaccine at intervals of 1 mo. A second group was given 250 CCA units with an interval of 2 mo between the first and second dose and an interval of 1 mo between the second and third dose. A slightly better antibody response was obtained with this schedule. In a second experiment, infants 6-9 mo of age were given an initial series of either 300 CCA units of aqueous or aluminum phosphate adsorbed hemagglutinin vaccine. These infants exhibited a better antibody response than younger ones. When after 9-12 mo a third dose of the same vaccine was given, a secondary response was achieved. The response of infants who received adsorbed vaccine was slightly higher. The response of infants to polyvalent influenza hemagglutinin is similar to their response to other inactivated vaccines.

Antibody Response to Influenza Virus Enzyme in Man

Antineuraminidase antibody levels were mea.. sured by inhibition of hemagglutination employing recombinant strain X-15 in paired sera of human subjects naturally infected with influenza AO, and A2, strains and in sets of sera obtained from persons vaccinated with swine, AO, A1, or A2 monovalent vaccines. The antineuraminidase antibody increase found with the X-15 test after infection and vaccination was shown to be subtype specific for A2 enzymes. The frequency and mag.. nitude antibody increase after both types of antigenic experience was similar. An ether-extracted hemagglutinin vaccine was, as efficient as its whole virus counterpart in stimulating antienzyme antibodies. Reinforcement of antienzyme antibody levels and antigenic broadening of the antibody response on repeated exposures was demonstrated.

Effect of AlPO4 on Antibody Response

Summary Groups of mice were inoculated once with graded dilutions of either aluminum phosphate adsorbed or aqueous influenza hemagglutinin vaccine containing A2/Taiwan/1/64 as a primary immunization. Sixty days later each group was divided: one half was given aqueous vaccine and the other, aluminum phosphate adsorbed hemagglutinin. The dose was the same strength as the first. The postbooster antibody response was higher in those groups who had received mineral carrier adsorbed vaccine as the first inoculation (p = .01) indicating that aluminum phosphate adsorbed hemagglutinin is a better conditioner for the secondary response than is aqueous vaccine.

Comparison of HI and HAdI Antibody Response of Military Recruits to Monovalent Vaccines.

Summary A comparison of the antibody response of humans vaccinated with either A2/Japan/305/57 or A2/Japan/170/62 monovalent vaccine as measured by HAdI and HI techniques has been presented. Although the HAdI antibody titers were higher the information obtained from comparisons of the frequency of antibody response and of mean fold titer increases was the same for both methods. Thus, it would appear from this experience that the HAdI technique offers no great advantage for the selection of vaccine strains when the criterion is the antibody response in man. In addition, it should be pointed out that the HAdI test is a more laborious, expensive and time consuming procedure than the HI method.