Alberto Dessy

Polymeric nanoparticles for hemoglobin-based oxygen carriers.

This article reports on the current status of the research on blood substitutes with particular attention on hemoglobin-based oxygen carriers (HBOCs). Insights on the physiological role of hemoglobin are reported in the view of the development of both acellular and cellular hemoglobin-based oxygen carriers. Attention is then focused on biocompatible polymeric materials that find application as matrices for cellular based HBOCs and on the strategies employed to avoid methemoglobin formation. Results are reported regarding the use of bioerodible polymeric matrices based on hemiesters of alternating copolymer (maleic anhydride-co-butyl vinyl ether) for the preparation of hemoglobin loaded nanoparticles.

Hemoglobin loaded polymeric nanoparticles: Preparation and characterizations

In the present work polymeric nanoparticles based on Poly (maleic anhydride-alt-butyl vinyl ether) 5% grafted with m-PEG (2000) and 95% grafted with 2-methoxyethanol (VAM41-PEG) were loaded with human hemoglobin (Hb) and characterized from a physicochemical point of view. The assessment of structural and functional features of the loaded Hb was performed and the effect of the introduction of different reducing agents as aimed at minimizing Hb oxidation during the nanoparticles formulation process, was also investigated. Nanoparticles possessing an average diameter of 138±10 nm and physicochemical features suitable for this kind of application were successfully obtained. Although the oxidation of the protein was not avoided during its loading into nanoparticles, the presence of acidic moieties in the polymeric structure is proposed to be directly involved in the protein inactivation mechanism.

Dead Sea Minerals loaded polymeric nanoparticles

Therapeutic properties of Dead Sea Water (DSW) in the treatment of skin diseases such as atopic dermatitis, psoriasis and photo aging UV damaged skin have been well established. DSW is in fact rich in minerals such as calcium, magnesium, sodium, potassium, zinc and strontium which are known to exploit anti-inflammatory effects and to promote skin barrier recovery. In order to develop a Dead Sea Minerals (DSM) based drug delivery system for topical therapy of skin diseases, polymeric nanoparticles based on Poly (maleic anhydride-alt-butyl vinyl ether) 5% grafted with monomethoxy poly(ethyleneglycol) 2000 MW (PEG) and 95% grafted with 2-methoxyethanol (VAM41-PEG) loaded with DSM were prepared by means of a combined miniemulsion/solvent evaporation process. The resulting nanoparticles were characterized in terms of dimension, morphology, biocompatibility, salt content and release. Cytocompatible spherical nanoparticles possessing an average diameter of about 300 nm, a time controlled drug release profile and a high formulation yield were obtained.

Magnetism and spin dynamics of novel encapsulated iron oxide superparamagnetic nanoparticles

Encapsulated Fe3O4 nanoparticles of average diameters d = 12 nm are obtained by coprecipitation, in the presence of 2-methoxyethanol hemiester of poly(maleic anhydride-alt-butyl vinyl ether) 5% grafted with poly(ethylene glycol) (VP-MAG nanoparticles). A complete characterization of nude and encapsulated nanoparticles through structural techniques (namely XRD, TEM, SEM), Raman spectroscopy and magnetic measurements has been performed. These nanoparticles compared with commercial compounds (ENDOREM®) present superparamagnetic behavior and nuclear relaxivities that make them promising as magnetic resonance imaging (MRI) contrast agents (CAs). We found that our nanostructures exhibit r2 relaxivity higher than those of commercial CAs over the whole frequency range. The MRI efficiency of our samples was related to their microstructural and magnetic properties.

Preparation and characterization of biodegradable amphiphilic polymers and nanoparticles with high protein-loading capacity

Multiblock copolymers containing carboxyl groups in the side-chains and at the chain ends were prepared from ABA triblock copolymers of ε-caprolactone, or lactide (as A block), and ethylene glycol (as B block). ABAn multiblock copolymers were prepared after chain-end functionalization and chain extension with pyromellitic dianhydride. A series of polymers were synthesized by varying the poly(ethylene glycol) and polyester molecular weight and the chirality of the lactide. Nuclear magnetic resonance analysis was used to confirm free carboxyl groups in the polymer backbone and at the chain ends. Thermal analysis indicated that the presence of pyromellitic dianhydride residues interfered not only with the formation of crystalline phases but also with the thermal degradation of chain-extended polymers. The biocompatibility of these amphiphilic polymers as evaluated with mouse embryo fibroblasts was acceptable. Both the parent ABA triblock copolymers and the carboxylated polymers were processed into nanoparticles. Depending on the polymer structure and reaction conditions, a narrow size nanoparticle distribution from ~10 to 250 nm was obtained. The nanoparticles were loaded with 60%–90% albumin and released 80%–90% of the albumin absorbed. Overall, this system was found to be well suited for the preparation of high-capacity injectable protein drug delivery.