Alejandro Costantini

Influence of tillage systems on biological properties of a Typic Argiudoll soil under continuous maize in central Argentina

Abstract Farmers are increasingly using zero tillage in Central Argentina to replace other tillage systems. Intensive tillage decreases soil organic matter content and causes physical degradation. The objective of this work was to evaluate changes in some soil biological properties induced by different tillage systems. A 6 year experiment in which continuous maize ( Zea mays L.) was grown using three tillage systems (conventional tillage, reduced tillage and zero tillage) was carried out at Cordoba Province, Argentina, on a Typic Argiudoll. Variations in total organic C content, microbial biomass C, metabolic quotient (qCO 2 ) and the proportion of the organic C present in the microbial biomass were evaluated at two sampling depths (0–5 and 5–15 cm). Additional samples from a nearby site (undisturbed grassland) were also taken and considered as a control. Concentrations of soil organic C and microbial biomass C were higher under zero tillage as compared with conventional tillage, at the 0–5 cm soil depth. Differences were not evident among tillage systems at the 5–15 cm soil depth. An analysis of the microbial biomass C content, in relation to the organic C, revealed higher values at the 0–5 cm soil depth only for those systems which provoke less disturbance of the soil (i.e. reduced tillage and zero tillage). Significantly greater amounts of CO 2 C were released from zero tillage and reduced tillage soils than from conventionally tilled soils. This release was positively correlated with microbial biomass C. qCO 2 values were not significantly different between tillage systems. Zero tillage proved to be more efficient in the conservation of organic C and microbial biomass C. The tillage systems impact on respiration was due to its effect on the microbial biomass.

A comparison of indexing methods to evaluate quality of soils: the role of soil microbiological properties

The study evaluates and compares two procedures for selecting soil quality indicators (used for the construction of soil quality indices, SQI) by using diverse chemical, physical, and biological properties, and evaluates the role of soil microbiological properties in the construction of SQI. Different soil environments were selected from an extensive agricultural production site in the rolling pampa, Buenos Aires, Argentina. The plots included an undisturbed soil, a grassland soil, and continuous tilled soils with four different surface horizon depths (25, 23, 19, and 14 cm). Various properties were measured, and a minimum dataset was chosen by principal component analysis (PCA) considering all measured soil properties together (procedure A), or the PCA was performed separately according to classification as physical, chemical, or biological soil properties (procedure B). The measured soil properties involved physical, chemical, and biochemical properties determined by standard protocols used in routine laboratory analysis (simple SQI, SSQI) or more laborious protocols to determine microbial community structure and function by phospholipid fatty acid (PLFA) and catabolic response profile (CRP), respectively (complex SQI, CSQI). The selected properties were linearly normalised and integrated by the weight additive method to calculate SSQI A, SSQI B, CSQI A, and CSQI B indices. Two microbiological SQI (MSQI) were also calculated; MSQI 1 considered only biological properties according to the procedure used for calculating SQI; MSQI 2 was calculated by considering three selected microbiological parameters representing the size (microbial biomass carbon), activity (soil basal respiration), and functional diversity (evenness, determined by CRP) of the microbial communities. All of the constructed indices show the same differences among the study sites. The inclusion of CRP and PLFA data in the indices slightly increased, or did not increase, the index sensitivity. Microbiological indices had the same sensitivity as the indices integrated by physical, chemical, and biological properties. An evaluation of the SQI constructed by both procedures found no difference in sensitivity. However, SQI constructed by procedure B allowed evaluation of the effects of management practices on physical, chemical, and biological soil properties.

Soil nitrous oxide emissions under different management practices in the semiarid region of the Argentinian Pampas

The aim of this study was to analyze the influence of different crop sequences (soybean-corn and soybean–soybean) and tillage systems (no tillage and reduced tillage) on nitrous oxide (N2O) soil emissions under field conditions. The experiment was carried out in Manfredi, Córdoba province, Argentina on an Entic Haplustoll and N2O emissions were measured in the field during a year. N2O fluxes were low during winter, but in late spring it peaked. For fallow, N-NO3-content was the most important variable to explain N2O emissions. For growing period water-filled pores was the main variable explaining N2O emissions. Nitrogen fertilization of corn crop increased N2O-N emissions, whereas no significant differences were found due to the tillage system. Measured annual N2O-N emissions were generally lower than those calculated using the methodology proposed by the Intergovernmental Panel on Climate Change.

The effect of different soil management procedures on carbon cycle components in an Entic Hapludoll

Abstract Sampling on different plots of a field located in Pehuajo, Buenos Aires Province, Argentina, on an Entic Hapludoll, was conducted to determine the: effect of different soil management procedures on carbon cycle components. The plots studied were: A) eight years of mixed pasture followed by oat (Avena saliva L.) crop, B) five years of mixed pasture followed by maize (Zea mays L.) with direct drilling, and C) five years of mixed pasture followed by maize crop with conventional tillage. The sampling was done during the month of June 1994. By that time, plot A was in oat production and plots B and C were in fallow. Microbial biomass carbon (MBC) values differed significantly between the three plots, whereas no differences were found in the organic carbon (OC) content. Plots B and C differed both in respiration and qCO2 (the ratio between C‐CO2 released by respioration and the microbial biomass C). For the latter, no differences had been found as a function of the tillage system in a previous work tha...

Seasonal changes in the microbial biomass of Brazilian soils with different organic matter contents

Abstract Carbon and nitrogen levels of microbial biomass were studied in four plots located in Rio de Janeiro State, Brazil. Two samplings were carried out, the first one, on November 1992 when rainfall was high which led the soil to high levels of moisture, and the second one on March 1993 when there was a decrease in rainfall coupled with high temperatures. Microbial carbon (MBC) and microbial nitrogen (MBN) assessments were done by the fumigation‐extraction method. The results showed significant differences for MBC and MBN between the sampling times and between different plots. Moreover, MBN showed differences as a function of sampling depth. In the 1993 sampling, developed under moisture conditions of soil which promoted the mineralization of organic matter, lesser values of MBC and MBN were found, whereas there was no difference in the organic carbon content. This fact shows a major sensitivity of biomass measurements to reflect changes which occur in the soil organic matter content. Significant corr...

Distribution and vertical stratification of carbon and nitrogen in soil under different managements in the pampean region of Argentina

One of the expected benefits of no-tillage systems is a higher rate of soil C sequestration. However, higher C retention in soil is not always apparent when no-tillage is applied, due e.g., to substantial differences in soil type and initial C content. The main purpose of this study was to evaluate the potential of no-tillage management to increase the stock of total organic C in soils of the Pampas region in Argentina. Forty crop fields under no-tillage and conventional tillage systems and seven undisturbed soils were sampled. Total organic C, total N, their fractions and stratification ratios and the C storage capacity of the soils under different managements were assessed in samples to a depth of 30 cm, in three layers (0-5, 5-15 and 15-30 cm). The differences between the C pools of the undisturbed and cultivated soils were significant (p no-tillage > conventional tillage). Based on the stratification ratio of the labile C pool (0-5/5-15 cm), the untilled were separated from conventionally tilled areas. Much of the variation in potentially mineralizable C was explained by this active C fraction (R2 = 0.61) and by total organic C (R2 = 0.67). No-till soils did not accumulate more organic C than conventionally tilled soils in the 0-30 cm layer, but there was substantial stratification of total and active C pools at no till sites. If the C stratification ratio is really an indicator of soil quality, then the C storage potential of no-tillage would be greater than in conventional tillage, at least in the surface layers. Particulate organic C and potentially mineralizable C may be useful to evaluate variations in topsoil organic matter.

Organic Carbon Origin and Stock in Cultivated and Grassland Soil of the Argentine Pampa

Abstract A study was carried out in the Argentine Pampa. Plots under continuous maize and maize–wheat/soybean–soybean rotation were used. Three control plots on grassland with different undisturbed periods were also used. The objective was to show that C3 and C4 plants have a different effect on the quantity of carbon retained in the soil when different crop sequences are used. Total organic carbon was determined, and mass spectrometry techniques were used to assess the natural variation of the abundance of 13C and 12C to trace carbon fate in the soil. No differences were observed in the carbon stock at 90 cm deep across cultivated plots. Maize monoculture represented an important contribution to the soil organic matter when compared to the grassland areas, but the comparison through the initial δ13C from reference plots did not allow an assessment of the original soil carbon in the plot under rotation.

A comparison of indexing methods to evaluate quality of horticultural soils. Part II. Sensitivity of soil microbiological indicators

Soil is a non-renewable natural resource, considered as the basis for food production. Changes in soil properties may indicate potentially beneficial or degradative effects of a given management practice, so it is important to select the most sensitive soil properties to act as quality indicators. This research evaluated different approaches to selecting soil quality indicators in the construction of soil quality indices (SQIs). The sensitivity of integrative SQIs, constructed by considering diverse chemical, physical, and biological properties, was compared with biological SQIs, using only biochemical and microbiological indicators, to assess soil quality in an intensive horticultural production system under short- and long-term organic and conventional management. The results provided by the SQIs showed that plots under organic management had increase soil quality compared with the conventionally managed plots, independent of the number of years under production. The SQIs integrated by physical, chemical and biological indicators were more sensitive than indices composed only of biological indicators, as they did not reflect the physical properties of the studied plots. The organic amendments had a great influence on the microbial community; therefore, microbiological indices could not provide reliable information on soil quality in production systems with high inputs of organic materials.