Alessandro Brovelli
École nationale de l'aviation civile
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Applied and Environmental Microbiology | 2014
Elsa Lacroix; Alessandro Brovelli; David Andrew Barry; Christof Holliger
ABSTRACT In chloroethene-contaminated sites undergoing in situ bioremediation, groundwater acidification is a frequent problem in the source zone, and buffering strategies have to be implemented to maintain the pH in the neutral range. An alternative to conventional soluble buffers is silicate mineral particles as a long-term source of alkalinity. In previous studies, the buffering potentials of these minerals have been evaluated based on abiotic dissolution tests and geochemical modeling. In the present study, the buffering potentials of four silicate minerals (andradite, diopside, fayalite, and forsterite) were tested in batch cultures amended with tetrachloroethene (PCE) and inoculated with different organohalide-respiring consortia. Another objective of this study was to determine the influence of pH on the different steps of PCE dechlorination. The consortia showed significant differences in sensitivities toward acidic pH for the different dechlorination steps. Molecular analysis indicated that Dehalococcoides spp. that were present in all consortia were the most pH-sensitive organohalide-respiring guild members compared to Sulfurospirillum spp. and Dehalobacter spp. In batch cultures with silicate mineral particles as pH-buffering agents, all four minerals tested were able to maintain the pH in the appropriate range for reductive dechlorination of chloroethenes. However, complete dechlorination to ethene was observed only with forsterite, diopside, and fayalite. Dissolution of andradite increased the redox potential and did not allow dechlorination. With forsterite, diopside, and fayalite, dechlorination to ethene was observed but at much lower rates for the last two dechlorination steps than with the positive control. This indicated an inhibition effect of silicate minerals and/or their dissolution products on reductive dechlorination of cis-dichloroethene and vinyl chloride. Hence, despite the proven pH-buffering potential of silicate minerals, compatibility with the bacterial community involved in in situ bioremediation has to be carefully evaluated prior to their use for pH control at a specific site.
Archive | 2014
Elsa Lacroix; Alessandro Brovelli; David Andrew Barry; Christof Holliger
Hydrologic Discovery Through Physical Analysis - Honoring the Scientific Legacies of Wilfried H. Brutsaert and Jean-Yves Parlange | 2012
Seifeddine Jomaa; David Andrew Barry; Alessandro Brovelli; B. C. P. Heng; G. C. Sander; J.-Y. Parlange
SETAC Europe 21st Annual meeting | 2011
Luca Rossi; Alessandro Brovelli; Pierre Queloz; Dien Ho; David Andrew Barry
River corridor restoration conference 2011 | 2011
Alessandro Brovelli; Jordi Battle-Aguilar; David Andrew Barry
AGU Fall meeting 2011 | 2011
Seifeddine Jomaa; David Andrew Barry; Alessandro Brovelli; Peter Heng; G. C. Sander; Jean-Yves Parlange
AGU Fall Meeting | 2010
Alessandro Brovelli; Jordi Batlle-Aguilar; David Andrew Barry; Joerg Luster; Juna Shrestha; Pascal A. Niklaus
eophysical Research Abstracts, European Geosciences Union | 2008
Alessandro Brovelli; D. Andrew Barry
Geophysical Research Abstract, European Geoscience Union conference | 2008
Alessandro Brovelli; Serge Baechler; Luca Rossi; D. Andrew Barry
Geophysical Research Abstract, European Geoscience Union conference | 2008
Alessandro Brovelli; Flavio Malaguerra; D. Andrew Barry