Alexander Damm

Sun-induced fluorescence - a new probe of photosynthesis: First maps from the imaging spectrometer HyPlant

Variations in photosynthesis still cause substantial uncertainties in predicting photosynthetic CO2 uptake rates and monitoring plant stress. Changes in actual photosynthesis that are not related to greenness of vegetation are difficult to measure by reflectance based optical remote sensing techniques. Several activities are underway to evaluate the sun-induced fluorescence signal on the ground and on a coarse spatial scale using space-borne imaging spectrometers. Intermediate-scale observations using airborne-based imaging spectroscopy, which are critical to bridge the existing gap between small-scale field studies and global observations, are still insufficient. Here we present the first validated maps of sun-induced fluorescence in that critical, intermediate spatial resolution, employing the novel airborne imaging spectrometer HyPlant. HyPlant has an unprecedented spectral resolution, which allows for the first time quantifying sun-induced fluorescence fluxes in physical units according to the Fraunhofer Line Depth Principle that exploits solar and atmospheric absorption bands. Maps of sun-induced fluorescence show a large spatial variability between different vegetation types, which complement classical remote sensing approaches. Different crop types largely differ in emitting fluorescence that additionally changes within the seasonal cycle and thus may be related to the seasonal activation and deactivation of the photosynthetic machinery. We argue that sun-induced fluorescence emission is related to two processes: (i) the total absorbed radiation by photosynthetically active chlorophyll; and (ii) the functional status of actual photosynthesis and vegetation stress.

Red and far red Sun‐induced chlorophyll fluorescence as a measure of plant photosynthesis

Remote estimation of Sun-induced chlorophyll fluorescence emitted by terrestrial vegetation can provide an unparalleled opportunity to track spatiotemporal variations of photosynthetic efficiency. Here we provide the first direct experimental evidence that the two peaks of the chlorophyll fluorescence spectrum can be accurately mapped from high-resolution radiance spectra and that the signal is linked to variations in actual photosynthetic efficiency. Red and far red fluorescence measured using a novel airborne imaging spectrometer over a grass carpet treated with an herbicide known to inhibit photosynthesis was significantly higher than the corresponding signal from an equivalent untreated grass carpet. The reflectance signal of the two grass carpets was indistinguishable, confirming that the fast dynamic changes in fluorescence emission were related to variations in the functional status of actual photosynthesis induced by herbicide application. Our results from a controlled experiment at the local scale illustrate the potential for the global mapping of terrestrial photosynthesis through space-borne measurements of chlorophyll fluorescence.

APEX - current status, performance and validation concept

The Airborne Prism EXperiment (APEX) is an airborne pushbroom imaging spectrometer for Earth observation. Its products will become available in 2011. APEX is currently prepared for final acceptance configuration completing final hardware upgrades, refined calibration methodologies and test flights. APEX is composed of an airborne dispersive pushbroom imaging spectrometer, a Calibration Home Base (CHB) for instrument calibration and a data Processing and Archiving Facility (PAF) for operational product generation and delivery. A unique In-Flight Characterization (IFC) unit is integrated within the sensor optical head, providing pre- and post- data-acquisition characterization monitoring the instruments spectral and radiometric stability. This paper outlines the activities performed with a special focus on system calibration and validation procedures, as well as preliminary measurement results.

Correction of Reflectance Anisotropy Effects of Vegetation on Airborne Spectroscopy Data and Derived Products

Directional effects in airborne imaging spectrometer (IS) data are mainly caused by anisotropic reflectance behavior of surfaces, commonly described by bi-directional reflectance distribution functions (BRDF). The radiometric and spectral accuracy of IS data is known to be highly influenced by such effects, which prevents consistent comparison of products. Several models were developed to approximate surface reflectance anisotropy for multi-angular observations. Few studies were carried out using such models for airborne flight lines where only a single observation is available for each ground location. In the present work, we quantified and corrected reflectance anisotropy on a single airborne HyMap flight line using a Ross-Li model. We stratified the surface in two vegetation structural types (different in vertical structuring) using spectral angle mapping, to generate a structure dependent set of angular observations. We then derived a suite of products [indices (structure insensitive pigment index, normalized difference vegetation index, simple ratio index, and anthocyanin reflectance index) and inversion-based (SAIL/PROSPECT-leaf area index, Cw, Cdm, Cab)] from corrected and uncorrected images. Non-parametric analysis of variance (Kruskal-Wallis test) showed throughout significant improvements in products from corrected images. Data correction resulting in airborne nadir BRDF adjusted reflectance (aNBAR) showed uncertainty reductions from 60 to 100% (p-value = 0.05) as compared to uncorrected and nadir observations. Using sparse IS data acquisitions, the use of fully parametrized BRDF models is limited. Our normalization scheme is straightforward and can be applied with illumination and observation geometry being the only a priori information. We recommend aNBAR generation to precede any higher level airborne IS product generation based on reflectance data.

Sun-induced chlorophyll fluorescence from high-resolution imaging spectroscopy data to quantify spatio-temporal patterns of photosynthetic function in crop canopies

Passive detection of sun-induced chlorophyll fluorescence (SIF) using spectroscopy has been proposed as a proxy to quantify changes in photochemical efficiency at canopy level under natural light conditions. In this study, we explored the use of imaging spectroscopy to quantify spatio-temporal dynamics of SIF within crop canopies and its sensitivity to track patterns of photosynthetic activity originating from the interaction between vegetation structure and incoming radiation as well as variations in plant function. SIF was retrieved using the Fraunhofer Line Depth (FLD) principle from imaging spectroscopy data acquired at different time scales a few metres above several crop canopies growing under natural illumination. We report the first maps of canopy SIF in high spatial resolution. Changes of SIF were monitored at different time scales ranging from quick variations under induced stress conditions to seasonal dynamics. Natural changes were primarily determined by varying levels and distribution of photosynthetic active radiation (PAR). However, this relationship changed throughout the day demonstrating an additional physiological component modulating spatio-temporal patterns of SIF emission. We successfully used detailed SIF maps to track changes in the canopys photochemical activity under field conditions, providing a new tool to evaluate complex patterns of photosynthesis within the canopy.

Combining Sun-Induced Chlorophyll Fluorescence and Photochemical Reflectance Index Improves Diurnal Modeling of Gross Primary Productivity

Sun-induced chlorophyll fluorescence (F) is a novel remote sensing parameter providing an estimate of actual photosynthetic rates. A combination of this new observable and Monteith’s light use efficiency (LUE) concept was suggested for an advanced modeling of gross primary productivity (GPP). In this demonstration study, we evaluate the potential of both F and the more commonly used photochemical reflectance index (PRI) to approximate the LUE term in Monteith’s equation and eventually improve the forward modeling of GPP diurnals. Both F and the PRI were derived from ground and airborne based spectrometer measurements over two different crops. We demonstrate that approximating dynamic changes of LUE using F and PRI significantly improves the forward modeling of GPP diurnals. Especially in sugar beet, a changing photosynthetic efficiency during the day was traceable with F and incorporating F in the forward modeling significantly improved the estimation of GPP. Airborne data were projected to produce F and PRI maps for winter wheat and sugar beet fields over the course of one day. We detected a significant variability of both, F and the PRI within one field and particularly between fields. The variability of F and PRI was higher in sugar beet, which also showed a physiological down-regulation of leaf photosynthesis. Our results underline the potential of F to serve as a superior indicator for the actual efficiency of the photosynthetic machinery, which is linked to physiological responses of vegetation.

Sensing of Photosynthetic Activity of Crops

The light use efficiency of photosynthesis dynamically adapts to environmental factors and is one major factor determining crop yield. Optical remote sensing techniques have the potential to detect physiological and biochemical changes in plant ecosystems, and non-invasive detection of changes in photosynthetic energy conversion may be of great potential for managing agricultural production in a future bio-based economy. Here we give an overview on the principles of optical remote sensing in crop systems with a special emphasis on investigating hyperspectral reflectance data and the sun-induced fluorescence signal. Especially sun-induced fluorescence as a parameter, which becomes important in remote sensing research may have great potential quantifying the physiological status of the photosynthetic apparatus. Both remote sensing principles were applied during the CEFLES2 campaign in Southern France, where the structural and functional status of several crops was measured on the ground and using state-of-the-art optical remote sensing techniques. Sun-induced fluorescence measurements over a variety of crops showed that additional information can be retrieved also over dense canopies, where classical remote sensing signals often saturate. With a view to the future, we discuss how hyperspectral reflectance and sun-induced fluorescence can quantitatively be related to photosynthetic efficiency and help to measure and manage productivity of natural and agricultural ecosystems.

Field and Airborne Spectroscopy Cross Validation—Some Considerations

Field spectroscopy is increasingly used in various fields of science: either as a research tool in its own right or in support of airborne- or space-based optical instruments for calibration or validation purposes. Yet, while the use of the instruments appears deceptively simple, the processes of light and surface interactions are complex to be measured in full and are further complicated by the multidimensionality of the measurement process. This study exemplifies the cross validation of in situ point spectroscopy and airborne imaging spectroscopy data across all processing stages within the spectroscopy information hierarchy using data from an experiment focused on vegetation. In support of this endeavor, this study compiles the fundamentals of spectroscopy, the challenges inherent to field and airborne spectroscopy, and the best practices proposed by the field spectroscopy community. This combination of theory and case study shall enable the reader to develop an understanding of 1) some of the commonly involved sources of errors and uncertainties, 2) the techniques to collect high-quality spectra under natural illumination conditions, and 3) the importance of appropriate metadata collection to increase the long-term usability and value of spectral data.

Evaluation of gross primary production (GPP) variability over several ecosystems in Switzerland using sun-induced chlorophyll fluorescence derived from APEX data

Plant photosynthesis mediates about 60Gt of the carbon uptake by vegetated ecosystems and is considered to be a critical component of the terrestrial carbon cycle. Photosynthesis is, however, a highly adaptive process and lack of knowledge on its dynamic causes uncertainties in current carbon budgets. New remote sensing approaches allow measuring the chlorophyll fluorescence signal (FS) and hold the potential to directly assess ecosystem photosynthesis and related carbon assimilation rates (i.e., gross primary production (GPP)). This study provides one of the first spatial investigations of GPP at local/regional scale. FS was retrieved from data of the new imaging spectrometer APEX (Airborne Prism EXperiment) and used to model GPP. Spatial variations of GPP were investigated for five major ecosystems in Switzerland. Results of this study are considered as important, e.g., for the development of ESAs (European Space Agency) FLEX (FLuorescence Explorer) mission, for investigating functional ecosystem responses to environmental properties, or for evaluating common ecosystem monitoring approaches (e.g., eddy flux tower).

Cross-Comparison of Albedo Products for Glacier Surfaces Derived from Airborne and Satellite (Sentinel-2 and Landsat 8) Optical Data

Surface albedo partitions the amount of energy received by glacier surfaces from shortwave fluxes and modulates the energy available for melt processes. The ice-albedo feedback, influenced by the contamination of bare-ice surfaces with light-absorbing impurities, plays a major role in the melting of mountain glaciers in a warming climate. However, little is known about the spatial and temporal distribution and variability of bare-ice glacier surface albedo under changing conditions. In this study, we focus on two mountain glaciers located in the western Swiss Alps and perform a cross-comparison of different albedo products. We take advantage of high spectral and spatial resolution (284 bands, 2 m) imaging spectrometer data from the Airborne Prism Experiment (APEX) and investigate the applicability and potential of Sentinel-2 and Landsat 8 data to derive broadband albedo products. The performance of shortwave broadband albedo retrievals is tested and we assess the reliability of published narrow-to-broadband conversion algorithms. The resulting albedo products from the three sensors and different algorithms are further cross-compared. Moreover, the impact of the anisotropy correction is analysed depending on different surface types. While degradation of the spectral resolution impacted glacier-wide mean albedo by about 5%, reducing the spatial resolution resulted in changes of less than 1%. However, in any case, coarser spatial resolution was no longer able to represent small-scale variability of albedo on glacier surfaces. We discuss the implications when using Sentinel-2 and Landsat 8 to map dynamic glaciological processes and to monitor glacier surface albedo on larger spatial and more frequent temporal scales.