Alexander Hubmer

Role of calcium in photodynamically induced cell damage of human fibroblasts.

Photodynamically induced changes in the cytoplasmic free calcium concentration ([Ca2+]i) and its role in cell damage were investigated in human skin fibroblasts using confocal laser microscopy. Fluorescence and absorbance spectrophotometry measurements indicate that the photosensitizer aluminum phthalocyanine tetrasulfonate (AlPcS4) binds to the plasma membrane and only after irradiation is able to enter the cells, causing massive morphologic alterations. Upon irradiation of sensitizer‐treated cells, the increase in [Ca2+]i is related to the amount of light and extracellular [Ca2+]e. The increase in [Ca2+]i was substantially reduced in the absence of [Ca2+]e. Cell damage or death after photodynamic treatment was prevented and shifted toward higher fluence by increasing [Ca2+]i at high [Ca2+]e and was greater at low [Ca2+]e. Application of Ca2+ channel blockers, such as Co2+, Cd2+ or verapamil, could not prevent the increase of [Ca2+]i. Our results indicate that activation of the photosensitizer, AlPcS4, causes an influx of Ca2+, which protects cells from photodamage. At low [Ca2+]e and high fluence values, release of Ca2+ from internal stores probably as a protective measure occurs in order to increase the [Ca2+]i.

Accumulation of anthropogenic radionuclides in cryoconites on Alpine glaciers.

Cryoconites are airborne sediments which accumulate on the surface of glaciers. In samples of cryoconites a temperate Austrian glacier high activity concentrations of anthropogenic radionuclides were found, which stem from global and Chernobyl fallouts. Radionuclides identified were (137)Cs, (134)Cs, (238)Pu, (239+240)Pu, (90)Sr, (241)Am, (60)Co, (154)Eu, (207)Bi, and (125)Sb. Given the approximately known isotopic ratios, Cs and Pu can be separated into the contributions of either source of origin. Published (137)Cs/(134)Cs and (239+240)Pu/(238)Pu ratios were used for the discrimination of the Dachstein-glacier cryoconites according to their origin from global or Chernobyl fallout. Two different groups of cryoconites were identified, an older population dominated by nuclear weapons fallout and a younger one with predominant Chernobyl fallout. With those data a simple model was formulated to demonstrate the transition and mixing of these two populations on the glacier surface.

90Sr and 137Cs in environmental samples from dolon near the semipalatinsk nuclear test site

The (90)Sr and (137)Cs activities of soil, plant, and milk samples from the village of Dolon, located close to the Semipalatinsk Nuclear Test Site in Kazakhstan, were determined. The areal deposition at the nine sampling sites is in the range of <500 to 6,100 Bq m(-2) and 300 to 7,900 Bq m-2 for (90)Sr and (137)Cs, respectively. Similar values have been reported in the literature. At some of the sites both nuclides mainly have remained in the top 6 cm of the soil profiles; at others they were partly transported into deeper soil layers since the deposition. For most of the samples the (90)Sr yield after destruction of the soil matrix is significantly higher than after extracting with 6 M HCl indicating that (90)Sr is partly associated with fused silicates. The low mean (90)Sr activity concentrations of vegetation samples (14 Bq kg(-1) dw) and milk samples (0.05 Bq kg(-1) fw) suggest that this has favorable consequences in terms of limiting its bioavailability.

Fallout strontium and caesium transfer from vegetation to cow milk at two lowland and two Alpine pastures.

Vegetation-to-milk transfer coefficients for 137Cs, 90Sr and stable strontium were determined for cows grazing on two intensively managed lowland pastures and two Alpine pastures in Austria. The 90Sr transfer coefficient at the four pastures ranged from 0.0005 to 0.0012 dl-1 and correlated with the stable strontium transfer coefficient (0.0006-0.0013 dl-1) with the lower values found on the intensively managed pastures. The 137Cs transfer coefficient ranged from 0.0009-0.0045 dl-1.

Occupational exposure to radon in treatment facilities of the radon-spa Badgastein, Austria

In the spa Badgastein, Austria, radon is used for therapeutic purposes for various diseases. Radon inhalation is applied in a thermal gallery with atmospheric radon concentrations up to 100 kBq/m3, elevated temperature up to 41°C, and humidity close to 100%, or in the form of radon baths where Rn is emanated from water with high natural Rn activity. Frequently, a combination of both treatment procedures is applied. The high environmental radon concentration levels in the thermal gallery, and in the various radon baths, can result in elevated radiation exposure levels, subject to significant local and temporal changes. Particularly in Rn-baths, the treatment procedures can result in high Rn levels or peaks during the use and replacement of Rn-water. For the assessment of the occupational exposure, a combination of long- and intermediate-term integrating Rn-measurements and continuous Rn and Rn-progeny measurements was used to investigate six different treatment facilities. Long-term integrating Rn-measurements, over a period of 1–13 months, provide reliable average Rn-concentration. However, these values cannot be used directly for the dose calculation of individuals due to the short-term fluctuation of the radiation exposure. Parallel integrating and continuous Rn-measurements and continuous Rn-daughter measurements were made to calculate personal occupancy weighting factors and Rn-equilibrium factors to determine potential alpha energy concentration exposure of daytime personnel.

Photodynamic effects on the nuclear envelope of human skin fibroblasts

Photodynamic effects on the nuclear envelope of human skin fibroblasts were investigated by confocal laser fluorescence microscopy and transmembrane resting potential measurements. The results show staining of the nuclear envelope after short incubation times with Photosan III, Photofrin II and haematoporphyrin derivative (HPD) enriched with monomers. Maximum staining was found at the centre of the nuclear envelope. The sequence of fluorescence intensity was HPD enriched with monomers > Photofrin II > Photosan III. After lethal treatment with Photosan III and tetrasulphonated aluminium chloride phthalocyanine, the nuclear transmembrane potential of the nuclear membrane decreased from -20 mV to about -10 mV with reference to the plasma membrane potential.

Radon in the Exhaled Air of Patients in Radon Therapy

In the Gastein valley, numerous facilities use radon for the treatment of various diseases either by exposure to radon in air or in radon rich thermal water. In this study, six test persons were exposed to radon thermal water in a bathtub and the time-dependent radon activity concentration in the exhaled air was recorded. At temperatures between 38°C and 40°C, the radon activity concentration in the water was about 900 kBq/m3 in a total volume of 600 l, where the patients were exposed for 20 min, while continuously sampling the exhaled air during the bathing and 20 min thereafter. After entering the bath, the exhaled radon activity concentration rapidly increased, reaching some kind of saturation after 20 min exposure. The radon activity concentration in the exhaled air was about 8000 Bq/m3 at the maximum, with higher concentrations for male test persons. The total radon transfer from water to the exhaled air was between 480 and 1000 Bq, which is equivalent to 0.08% and 0.2% of the radon in the water.