Alexander V. Kalachev

An ultrastructural study of phagocytosis and shrinkage in nutritive phagocytes of the sea urchin Anthocidaris crassispina

The ultrastructural mechanisms of waste-sperm phagocytosis and postspawning shrinkage were studied for accessory cells (nutritive phagocytes; NPs) of the sea urchin Anthocidaris crassispina. Sperm cells were phagocytosed by NPs; they penetrated into the cytoplasm of the NPs inside heterophagosomes formed by an invagination of the cell membrane. Single-sperm-containing heterophagosomes aggregated to form large multisperm heterophagosomes that were accompanied by cytoplasmic vesicles and lipids. Two types of vesicle, viz., Golgi-complex-derived electron-dense vesicles (“zymogen granules”) and smooth-endoplasmic-reticulum-derived electron-lucent vesicles, were incorporated within multisperm heterophagosomes. Completed multisperm heterophagosomes were transformed into electron-dense remnant bodies, the content of which underwent destruction, resulting in “empty” vacuoles inside the remnant body. The “empty” vacuoles were then compressed by the surrounding cytoplasm. Shrinkage of NPs occurred upon completion of sperm degeneration in gonad tubules. This process was undertaken by structures termed cell-size-reducing autolysosomes, which performed two types of autolysis, and resulted in the formation of “cheese-hole”-like vacuoles in the cytoplasm of NPs. Subsequent cytoplasmic compression of these vacuoles was required for the reduction in size of NPs, an essential event for remodeling the cell for the next gametogenetic cycle.

Selective resorption in nutritive phagocytes of the sea urchin Anthocidaris crassispina

Phagocytic resorption during spermatogenesis was studied in the sea urchin Anthocidaris crassispina. Nutritive phagocytes in gonad absorbed both waste sperm cells and residual bodies discarded from maturing spermatids, and these materials were subsequently compartmented in heterophagosomes. Based on 180 heterophagosomes examined by transmission electron microscopy, over 99% of heterophagosomes contained either residual bodies or sperm cells only. Simultaneous resorption of sperm cells and residual bodies in a heterophagosome was uncommon, with only approximately 0.56% occurrence, suggesting that heterophagosomes have a selective resorption ability in nutritive phagocytes.

Biplexiform ganglion cells contact photoreceptors in the retina of the greenling Hexagrammos octogrammus.

The biplexiform cell (bpxRGC) is a relatively and recently discovered type of retinal ganglion cells. Like “ordinary” ganglion cells, bpxRGCs have dendrites arborizing within the inner plexiform layer. However, as distinct from other ganglion cells, they have dendrites ascending to the outer plexiform layer. To date, bpxRGCs have been found in mammals, amphibians, and teleost fishes (Cook et al. [1996] Vis Neurosci 13:517–528). The mammalian and amphibian bpxRGCs form direct contacts with photoreceptors and may participate in rapid signal transmission to the brain (Mariani [1982] Science 216:1134–1136; Straznicky and Gábriel [1995] J Hirnforsch 36:135–141). The synaptic organization of teleost bpxRGCs has not been studied. We have studied the synaptic structure of bpxRGCs in the teleost fish Hexagrammos octogrammus. In the sclerad part of the outer plexiform layer, bpxRGC dendrites occurred among the elements in invaginated ribbon synapses (triads) in cone pedicles and rod spherules. Earlier, we showed that greenling bpxRGCs project to the optic tectum (Podugolnikova et al. [2002] Sensornye systemy 15:44–53). We suggest that greenling bpxRGCs participate in some of the tectum‐mediated reactions requiring a quick launch of visuomotor reflexes. Synapse 64:937–940, 2010.

Presence, character and number of accessory cells in holothurian male germinative epithelium: an ultrastructural study

Summary Accessory cell patterns were examined in the male gonads of the holothurians Apostichopus japonicus, Cucumaria japonica and Eupentacta fraudatrix. A. japonicus has a different type of somatic cell from the other two species. The general morphology of accessory cells is quite similar in C. japonica and E. fraudatrix, but there is a significant difference in the number of these cells in the germinative epithelium in these species. The possibility that individual features of accessory cell populations can be useful as an additional taxonomic character is discussed.

Microautophagy in nutritive phagocytes of sea urchins

Two types of cells were observed in germinative epithelium of male and female sea urchins: germ cells and somatic accessory cells; the latter referred to as nutritive phagocytes. At the onset of gametogenesis, nutritive phagocytes accumulate nutrients and greatly increase in their size. As gametogenesis progresses, the accumulated nutrients are transferred from nutritive phagocytes into developing gametes, and size of the nutritive phagocytes decreases. An electron microscopic study of nutritive phagocytes in sea urchins, Strongylocentrotus intermedius, at different stages of annual reproductive cycle showed for the first time that both macro- and microautophagy take place in nutritive phagocytes. Both processes occur simultaneously and regulate size and composition of nutritive phagocytes in male and female sea urchins. Nutritive phagocytes consume redundant cytoplasm via macroautophagy. Microautophagy is probably involved in consumption of redundant membranes that appear within nutritive phagocytes due to destruction of nutrient-storing globules, macroautophagy, and phagocytosis of germ cells or their remnants.

Resorption of Gametes in the Testes of the Sea Star Asterina pectinifera (Mueller et Troschel, 1842)

The process of resorption of spermatozoa is described in the testes of the starfish Asterina pectinifera. It is shown that the mode of resorption of the male gametes in this species is different from that in the starfish Asterias vulgaris (see Walker [17, 18]), A. amurensis, and Aphelasterias japonica (see Kas’yanov et al.

An ultrastructural study of testes permeability in sea urchins, Strongylocentrotus intermedius.

Permeability of testes in sea urchins, Strongylocentrotus intermedius, was investigated by using an electron-opaque tracer, lanthanum nitrate. This tracer is able to enter the basal compartment of germinative epithelium, where developing germ cells are located. However, its ability to penetrate the gonadal lumen was reduced. An incomplete permeability barrier between the basal compartment and the gonadal lumen is supposed to exist in testes of S. intermedius.

A brief summary of neuroendocrine regulation of reproduction in sea stars

Over than fifty years starfishes have been widely used as model for studying the mechanisms of cell cycle regulation, oocyte maturation and fertilization. Besides, significant work has been done to investigate the role of nervous system in the control of reproduction and spawning in these animals. Nowadays, sea stars represent one of the most thoroughly studied model for hormonal regulation of reproduction among invertebrates. However, while the general picture of neuroendocrine control of asteroid reproduction can be drawn easily, our knowledge concerning the details of this process still has some gaps. Filling these gaps is essential for studying the diversity of hormonal mechanisms involved in regulation of animal reproduction. The present paper aims to briefly summarize current data on hormonal regulation of reproduction in sea stars and to highlight existing gaps in our knowledge on the details of this process.

An ultrastructural study of accessory cells in the testes of the sea cucumber Chiridota pellucida (Holothuroidea: Apoda)

Summary The somatic accessory cells in the testes of the sea cucumber, Chiridota pellucida (Holothuroidea: Apoda), are flattened flagellated epithelial cells covering the basal lamina of the germinal epithelium. Their nucleus is irregular or elongated with a prominent nucleolus. The cytoplasm contains the Golgi complex, cisternae of rough and smooth endoplasmic reticulum, numerous electron-lucent vesicles, and lipid droplets. C. pellucida accessory cells differ in ultrastructure from those in other holothurians.

Phagocytic Activity of Accessory Cells in the Gonad of the Sea Urchin Strongylocentrotus nudus

The ultrastructural mechanism of postspawning sperm resorption in the testes of the sea urchin Strongylocentrotus nudus is described. Two types of phagosomes (containing sperm and containing residual bodies) are formed in the cytoplasm of nutritive phagocytes. The phagosomes fuse with electron-dense globules, and their contents are gradually destroyed. Afterward, phagosomes are transformed into electron-transparent vacuoles, which are finally compressed by the surrounding cytoplasm.