Alexandre Fortes Drummond

Association of functional gene polymorphism IL-1β in patients with external apical root resorption

INTRODUCTION External apical root resorption (EARR) is a frequent complication of orthodontic treatment. Interleukin 1beta (IL-1beta) is a potent stimulus for bone resorption and osteoclastic cell recruitment during orthodontic tooth movement. The purpose of this study was to assess the association between a polymorphism in the IL-1beta genes and EARR during orthodontic treatment. METHODS The sample included 61 Brazilian orthodontic patients, divided into 2 groups according to the presence (affected group, n = 23) or absence (control group, n = 38) of EARR in the central and lateral maxillary incisors in the posttreatment period. DNA was obtained from buccal swab cells. The polymorphism was analyzed by the polymerase chain reaction followed by digestion with restriction enzyme. The polymerase chain reaction products were analyzed in 10% polyacrylamide gel and stained with silver. RESULTS There were significant statistical differences (P <0.05) among the frequencies of the alleles and genotypes of the IL-1beta gene polymorphism between the affected and unaffected groups, suggesting that allele 1 predisposed the subjects to EARR (odds ratio = 4.0). CONCLUSIONS The polymorphism of the IL-1beta gene is associated with root resorption in the studied population.

The Dental Aesthetic Index and Dental Health Component of the Index of Orthodontic Treatment Need as Tools in Epidemiological Studies

The present study assesses the validity and reproducibility of two occlusal indices for epidemiological studies—the Dental Aesthetic Index (DAI) and the Dental Health Component of the Index of Orthodontic Treatment Need (DHC-IOTN) for the identification of orthodontic treatment needs. The total of 131 study models was examined by an examiner (orthodontic specialist) for the determination of the DAI and DHC-IOTN. Thirty days later, further assessment was performed to determine the reproducibility. The duration of each exam was measured in seconds with a stopwatch. The indices were compared by a panel of three experts in orthodontics to evaluate validity. The intra-examiner reliability evaluation resulted in an intraclass correlation coefficient of 0.89 for the DAI (95% CI = 0.64 to 1.0) and 0.87 for the DHC-IOTN (95% CI = 0.56 to 0.96). The time spent on the evaluation of the DHC-IOTN was less than the time spent on that of the DAI (P < 0.001). The accuracy of the indices, as reflected by the area under the receiver-operating characteristic curve, was 61% for the DAI (95% CI = 51 to 70; p = 0.037) and 67% for the DHC-IOTN (95% CI = 58 to 77; p = 0.001). Both indices presented good reproducibility and validity.

Esterilização em Ortodontia: eficácia do esterilizador com esferas de vidro

AIM: The aim of this research was to evaluate the efficiency of Steri® 350glass bead sterilizer, on the infection control of the active part of the orthodontic pliers. METHODS: Nine orthodontic pliers were sterilized in the steam autoclave at 121oC in 20 minutes and subsequently the active part of each plier was dipped in a culture for Bacillus stearothermophilus. Later, the active part of each plier was putted into the Steri® 350 in the following periods: 3, 5, 10, 15, 20, 30, and 40 seconds at 255oC to evaluate the efficiency of this method in the infection control. RESULTS AND CONCLUSIONS: This sterilization method has been effective to control the presence of Bacillus stearothermophilus on the active part of orthodontic pliers at 255oC in 10 seconds.

Risk variables of external apical root resorption during orthodontic treatment

INTRODUCTION: External apical root resorption (EARR) is an adverse outcome of the orthodontic treatment. So far, no single or associated factor has been identified as responsible for EARR due to tooth movement. OBJECTIVE: This study investigated the association of risk variables (age, gender, extraction for orthodontic treatment and Angle classification) with EARR and orthodontic treatment. METHOD: The sample (n=72) was divided into two groups according to presence (n=32) or absence (n=40) of EARR in maxillary central and lateral incisors after orthodontic treatment. RESULTS: There were no statistically significant differences in EARR according to age, gender, extraction or type of malocclusion (p>0.05). CONCLUSION: The risk variables examined were not associated with EARR in the study population.

Mini-implante como estratégia de tratamento para reabilitação protética temporária – relato de caso

A avulsao de incisivos permanentes gera um problema estetico e funcional para o paciente, e seu manejo clinico representa um desafio na odontologia. O sucesso do tratamento depende de varios fatores como a idade do paciente, a quantidade e qualidade ossea da regiao edentula, a discrepância ossea e o tipo de maloclusao encontrada. Ressalta-se a importância de minimizar efeitos colaterais nas unidades de ancoragem, garantindo ao mesmo tempo funcao e estetica ao longo dos tratamentos ortodontico e protetico, requeridos na maioria dos casos. O objetivo desse trabalho e apresentar atraves de um relato de caso clinico a versatilidade dos mini-implantes na reabilitacao temporaria de incisivos permanentes em individuos em fase de crescimento e desenvolvimento facial. Um menino de 9 anos que apresentava relacao de molar em classe I e mesializacao dos incisivos laterais, caninos e pre-molares superiores devido a ausencia dos elementos 11 e 21, que foram avulsionados, foi tratado; e ao final de 1 ano e 8 meses de tratamento, pode-se estabelecer uma intercuspidacao dentaria aceitavel e espaco adequado para confeccao das coroas provisorias sobre os parafusos. O acompanhamento radiografico foi realizado atraves de radiografias periapicais e panorâmicas. Na literatura os estudos mostram que os mini-implantes contribuem para a manutencao da quantidade e qualidade ossea do processo alveolar, reduzindo a necessidade de futuras cirurgias de enxerto antes da fixacao dos implantes osseointegrados. Dessa forma, esse caso mostrou que a ancoragem esqueletica com mini-implantes ortodonticos autorosqueaveis auxilia na reabilitacao de pacientes que ainda nao possuem uma idade ossea suficiente para receberem implantes dentarios osseointegrados.