Network


Latest external collaboration on country level. Dive into details by clicking on the dots.

Hotspot


Dive into the research topics where Alexey Khodjakov is active.

Publication


Featured researches published by Alexey Khodjakov.


Nature Reviews Molecular Cell Biology | 2010

Mechanisms of chromosome behaviour during mitosis

Claire E. Walczak; Shang Cai; Alexey Khodjakov

For over a century, scientists have strived to understand the mechanisms that govern the accurate segregation of chromosomes during mitosis. The most intriguing feature of this process, which is particularly prominent in higher eukaryotes, is the complex behaviour exhibited by the chromosomes. This behaviour is based on specific and highly regulated interactions between the chromosomes and spindle microtubules. Recent discoveries, enabled by high-resolution imaging combined with the various genetic, molecular, cell biological and chemical tools, support the idea that establishing and controlling the dynamic interaction between chromosomes and microtubules is a major factor in genomic fidelity.


Nature Cell Biology | 2009

Chromosome congression in the absence of kinetochore fibres

Shang Cai; Christopher B. O'Connell; Alexey Khodjakov; Claire E. Walczak

Proper chromosome congression (the process of aligning chromosomes on the spindle) contributes to accurate and faithful chromosome segregation. It is widely accepted that congression requires kinetochore fibres (K-fibres), microtubule bundles that extend from the kinetochores to spindle poles. Here, we demonstrate that chromosomes in human cells co-depleted of HSET (human kinesin-14) and hNuf2 (human Ndc80/Hec1-complex component) can congress to the metaphase plate in the absence of K-fibres. However, the chromosomes are not stably maintained at the metaphase plate under these conditions. Chromosome congression in HSET + hNuf2 co-depleted cells required the plus-end directed motor CENP-E (centromere protein E; kinesin-7 family member), which has been implicated in the gliding of mono-oriented kinetochores alongside adjacent K-fibres. Thus, proper end-on attachment of kinetochores to microtubules is not necessary for chromosome congression. Instead, our data support the idea that congression allows unattached chromosomes to move to the middle of the spindle where they have a higher probability of establishing connections with both spindle poles. These bi-oriented connections are also used to maintain stable chromosome alignment at the spindle equator.


Nature Cell Biology | 2010

Centromere tension: a divisive issue

Alexey Khodjakov; Jonathon Pines

It has been proposed that the spindle assembly checkpoint detects both unattached kinetochores and lack of tension between sister kinetochores when sister chromatids are not attached to opposite spindle poles. However, here we argue that there is only one signal — whether kinetochores are attached to microtubules or not — and this has implications for our understanding of both chromosome segregation and the control of genomic stability.


Cytoskeleton | 1997

A synergy of technologies: combining laser microsurgery with green fluorescent protein tagging.

Alexey Khodjakov; Richard W. Cole; Conly L. Rieder

When focused through an objective lens with a high numerical aperture, nanosecond pulses of high-intensity green (532-nm) laser light can be used to selectively destroy any cellular component whose boundaries can be defined by light microscopy. These components include, for example, chromosomes, spindle fibers, bundles of keratin, or actin filaments, mitochondria, vacuoles, and so forth. In addition, the definition of poorly resolved components can be enhanced for selective destruction by tagging one or more of their constituent proteins with green fluorescence protein (GFP). As a example we show that the centrosome in living PtK1 cells can be clearly defined, and then destroyed by green laser light, after transforming the cells with gamma-tubulin/GFP fusion protein. In some transformed cells it is even possible to target and selectively destroy just one of the centrioles.


Molecular Biology of the Cell | 2012

Concerted effort of centrosomal and Golgi-derived microtubules is required for proper Golgi complex assembly but not for maintenance.

Tatiana Vinogradova; Raja Paul; Ashley D. Grimaldi; Jadranka Loncarek; Paul M. Miller; Dmitry Yampolsky; Valentin Magidson; Alexey Khodjakov; Alex Mogilner; Irina Kaverina

Using computational modeling and laser microsurgery, we establish that neither the centrosomal microtubule array nor the Golgi-derived array is solely sufficient for correct Golgi assembly. Only the concerted effort of both MT arrays results in the integral, polarized Golgi complex necessary for polarized trafficking and cell motility.


Journal of Cell Science | 2004

Multiple mechanisms regulate NuMA dynamics at spindle poles

Olga P. Kisurina-Evgenieva; Gary J. Mack; Quansheng Du; Ian G. Macara; Alexey Khodjakov; Duane A. Compton

The large coiled-coil protein NuMA plays an essential role in organizing microtubule minus ends at spindle poles in vertebrate cells. Here, we use both in vivo and in vitro methods to examine NuMA dynamics at mitotic spindle poles. Using fluorescence recovery after photobleaching, we show that an exogenously expressed green-fluorescent-protein/NuMA fusion undergoes continuous exchange between soluble and spindle-associated pools in living cells. These dynamics require cellular energy and display an average half-time for fluorescence recovery of ∼3 minutes. To explore how NuMA dynamics at spindle poles is regulated, we exploited the association of NuMA with microtubule asters formed in mammalian mitotic extracts. Using a monoclonal antibody specific for human NuMA, we followed the fate of human NuMA associated with microtubule asters upon dilution with a hamster mitotic extract. Consistent with in vivo data, this assay shows that NuMA can be displaced from the core of pre-assembled asters into the soluble pool. The half-time of NuMA displacement from asters under these conditions is ∼5 minutes. Using this assay, we show that protein kinase activity and the NuMA-binding protein LGN regulate the dynamic exchange of NuMA on microtubule asters. Thus, the dynamic properties of NuMA are regulated by multiple mechanisms including protein phosphorylation and binding to the LGN protein, and the rate of exchange between soluble and microtubule-associated pools suggests that NuMA associates with an insoluble matrix at spindle poles.


Journal of Biology | 2009

The nature of cell-cycle checkpoints: facts and fallacies

Alexey Khodjakov; Conly L. Rieder

The concept of checkpoint controls revolutionized our understanding of the cell cycle. Here we revisit the defining features of checkpoints and argue that failure to properly appreciate the concept is leading to misinterpretation of experimental results. We illustrate, using the mitotic checkpoint, problems that can arise from a failure to respect strict definitions and precise terminology.


Progress in cell cycle research | 1997

Mitosis and checkpoints that control progression through mitosis in vertebrate somatic cells

Conly L. Rieder; Alexey Khodjakov

During mitosis in vertebrates the sister kinetochores on each replicated chromosome interact with two separating arrays of astral microtubules to form a bipolar spindle that produces and/or directs the forces for chromosome motion. In order to ensure faithful chromosome segregation cells have evolved mechanisms that delay progress into and out of mitosis until certain events are completed. At least two of these mitotic checkpoint controls can be identified in vertebrates. The first prevents nuclear envelope breakdown, and thus spindle formation, when the integrity of some nuclear component(s) is compromised. The second prevents chromosome disjunction and exit from mitosis until all of the kinetochores are attached to the spindle.


Journal of Muscle Research and Cell Motility | 2002

Dynamic microtubules in Dictyostelium

Michael P. Koonce; Alexey Khodjakov

The term ‘microtubule dynamics’ is often used to describe assembly/disassembly characteristics of this important cytoskeletal polymer. The ability to image microtubules in live Dictyostelium cells has revealed additional dynamic components, acting on the individual assembled tubules. At least two separate forces are involved, in generation of pronounced bending motions during interphase and in creating tension with the cell cortex. This review attempts to summarize what is known about conventional microtubule dynamics in Dictyostelium as well as to describe these two additional motility components. We propose that these forces are important both in maintaining the overall structure of the microtubule array and in supporting intracellular traffic.


PLOS Biology | 2007

The spindle pole bodies facilitate nuclear envelope division during closed mitosis in fission yeast.

Liling Zheng; Cindi L. Schwartz; Valentin Magidson; Alexey Khodjakov; Snezhana Oliferenko

Many organisms divide chromosomes within the confines of the nuclear envelope (NE) in a process known as closed mitosis. Thus, they must ensure coordination between segregation of the genetic material and division of the NE itself. Although many years of work have led to a reasonably clear understanding of mitotic spindle function in chromosome segregation, the NE division mechanism remains obscure. Here, we show that fission yeast cells overexpressing the transforming acid coiled coil (TACC)-related protein, Mia1p/Alp7p, failed to separate the spindle pole bodies (SPBs) at the onset of mitosis, but could assemble acentrosomal bipolar and antiparallel spindle structures. Most of these cells arrested in anaphase with fully extended spindles and nonsegregated chromosomes. Spindle poles that lacked the SPBs did not lead the division of the NE during spindle elongation, but deformed it, trapping the chromosomes within. When the SPBs were severed by laser microsurgery in wild-type cells, we observed analogous deformations of the NE by elongating spindle remnants, resulting in NE division failure. Analysis of dis1Δ cells that elongate spindles despite unattached kinetochores indicated that the SPBs were required for maintaining nuclear shape at anaphase onset. Strikingly, when the NE was disassembled by utilizing a temperature-sensitive allele of the Ran GEF, Pim1p, the abnormal spindles induced by Mia1p overexpression were capable of segregating sister chromatids to daughter cells, suggesting that the failure to divide the NE prevents chromosome partitioning. Our results imply that the SPBs preclude deformation of the NE during spindle elongation and thus serve as specialized structures enabling nuclear division during closed mitosis in fission yeast.

Collaboration


Dive into the Alexey Khodjakov's collaboration.

Top Co-Authors

Avatar
Top Co-Authors

Avatar

Greenfield Sluder

University of Massachusetts Medical School

View shared research outputs
Top Co-Authors

Avatar

Patricia Wadsworth

University of Massachusetts Amherst

View shared research outputs
Top Co-Authors

Avatar
Top Co-Authors

Avatar

Claire E. Walczak

Indiana University Bloomington

View shared research outputs
Top Co-Authors

Avatar

E. D. Salmon

University of North Carolina at Chapel Hill

View shared research outputs
Top Co-Authors

Avatar
Top Co-Authors

Avatar

Valentin Magidson

New York State Department of Health

View shared research outputs
Top Co-Authors

Avatar
Top Co-Authors

Avatar
Researchain Logo
Decentralizing Knowledge