Alexis J. Kniazeff

Effect of Neuraminidase Pretreatment on the Susceptibility of Normal and Transformed Mammalian Cells to Bovine Enterovirus 261

TAYLOR et al. reported regression of ascites and solid tumours in mice inoculated with bovine enterovirus 1 (BEV-1)1 without any observable pathological effect on the animals. The basis of the oncolytic specificity was associated with the adsorption spectrum of the virus2,3. The specificity was also expressed in vitro since virus transformed and oncogenic cells were susceptible to BEV-1, in contrast to cells of un-transformed lines and of primary cultures, which were resistant4.

Debut and Accumulation of Centric Fusion Products: An Index to Age of Certain Cell Lines

Fourteen cell lines derived from cattle ( Bos taurus ), sheep ( Ovis arks ), goat ( Capra hircus ) and dog ( Canis familiaris ) were

Chromatin Bridges and Origin of Multinucleate Cells in a Bovine Testicular Cell Line

In the course of cytological observations of a cell line of bovine testicular origin since its initiation, many chromatin bridges and binucleate cells were noticed following the apparently spontaneous occurrence of chromatid fragmentation and formation of dicentric chromosomes. Subsequently, the frequency of binucleate cells and their respective nuclear sizes were recorded from permanent slides of material of passages made prior to, during, and after the incidence of chromatid fragmentation. Since the frequency of binucleate cells rose simultaneously with that of the chromatin bridges, and since the same nuclear size was involved in most cases, the frequency of chromatin bridges was taken as an index of their contribution to the production of binucleate cells following the period of chromatid fragmentation.

Peculiarities of ovine cells in culture

SummaryThe chromosome complement of two ovine (Ovis aries L.) kidney cell lines are described. Nuclei of MDOK, the older cell line, are generally larger than those of OK cells, they are hypo-tetraploid and show considerable chromosomal irregularities both in structure and behavior. The OK line observed since its initiation, is now hyper-diploid and exhibits a gradual accumulation of chromosomes. Both cell lines have in common the fact that they now maintain a larger proportion of chromosomes with interstitial centromeres than telocentric chromosomes. This observation parallels similar ones made earlier in bovine cells. However, the processes whereby this condition arose in the respective cultures are believed to be different and are discussed.

On the karyotype of the tahr Hemitragus jemlahicus and the Y-chromosome of goats and sheep

Somatic chromosomes of a female and male Himalayan thar, Hemitragus jemlahicus (H. Smith) are described. The diploid number is 48, there are 12 atelocentric and 34 telocentric autosomes in both sexes, the X-chromosome is meta- or submetacentric. The morphological appearance of the Y-chromosome is compared with that of other bovid species including recent observations on the goat Capra hircus.