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Dive into the research topics where Alfred A. Pan is active.

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Featured researches published by Alfred A. Pan.


Journal of Virological Methods | 2008

Development of a high-throughput Alamar blue assay for the determination of influenza virus infectious dose, serum antivirus neutralization titer and virus ca/ts phenotype.

Chengjun Mo; Ryan Yamagata; Alfred A. Pan; Jhansi Reddy; Nisha Hazari; Gregory Duke

FluMist is an intranasal influenza live vaccine containing two Influenza A strains (currently H1N1 and H3N2) and one B strain (Yamagata or Victoria lineage). Characterization of the vaccine requires determination of the median tissue culture infectious dose (TCID(50)) titer, serum antivirus neutralization titer and vaccine cold adapted/temperature sensitive (ca/ts) phenotype. Visual cytopathic effect (CPE) readings are used widely in viral assays, but these are subjective and labor intensive. In response to the need for an efficient, inexpensive and high-throughput assay, a 96-well microplate assay was developed that uses Alamar blue dye staining as a replacement for CPE observation in the determination of influenza virus infectious dose, serum antivirus neutralization titer and virus ca/ts phenotype. Relative operating characteristic curves verified that there was a clear distinction between the fluorescence readings of the Alamar blue stained CPE positive and CPE negative wells. Virus titer was determined by use of both Alamar blue staining and CPE-based TCID(50) assays for wild-type and FluMist influenza vaccine strains as well as a plasmid-rescued influenza FluMist A strain containing a H5N1 derived hemmaglutinin and neuramidinase. Correlation of the two assays was measured by regression analysis and resulted in R(2) values of 0.814 (Influenza A), 0.983 (Influenza B) and 1.000 (H5N1), respectively. Serum microneutralization as well as virus ca/ts phenotype assays also showed a high concordance between readings based on CPE observation and Alamar blue staining. The Alamar blue dye assay is user friendly, environmentally safe and sensitive. Also, it is adaptable to automation, which could provide a high-throughput platform for analysis of pre-clinical and clinical samples.


Journal of Virological Methods | 2007

Biophysical characterization of influenza virus subpopulations using field flow fractionation and multiangle light scattering: Correlation of particle counts, size distribution and infectivity

Ziping Wei; Matt Mcevoy; Vladimir Razinkov; Alla Polozova; Elizabeth Li; Jose Casas-Finet; Guillermo I. Tous; Palani Balu; Alfred A. Pan; Harshvardhan Mehta; Mark Schenerman


Archive | 2004

Methods of producing inflenza vaccine compositions

George Robert Trager; George Kemble; Richard Schwartz; Harshvardhan Mehta; Vu Truong-Le; Zhongying Chen; Alfred A. Pan; Eric Tsao; Chiaoyin Kathy Wang; Luisa Yee; Palani Balu


Archive | 1993

Assay for chagas' disease and reagents for its use

Martin A. Winkler; Alfred A. Pan


Archive | 1994

Assay for Trypansoma cruzi antibodies which specifically bind three different antigens

Martin A. Winkler; Alfred A. Pan


Labmedicine | 1997

The Threat of Chagas’ Disease in Transfusion Medicine: The Presence of Antibodies to Trypanosoma cruzi in the US Blood Supply

Alfred A. Pan; Martin A. Winkler


Archive | 1996

Process for purifying the Gp60/50 antigen of T. cruzi

Martin A. Winkler; Alfred A. Pan


Archive | 1995

Process for linking an antigenic glycolipid of T. cruzi to a protein carrier

Martin A. Winkler; Alfred A. Pan


Molecular Immunology | 2010

Is there a link between the human TRIM21 and Trypanosoma cruzi Clone 36 genes in Chagas’ disease?

Martin A. Winkler; Alfred A. Pan


Archive | 2004

Cross flow filtration in the production of stabilized influenza vaccine compositions

George Kemble; Luisa Yee; Chiaoyin Kathy Wang; Palani Balu; George Robert Trager; Richard Schwartz; Harshvardhan Mehta; Vu Truong-Le; Zhongying Chen; Alfred A. Pan; Eric Tsao

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