Alfredo Calleja

Nutritive evaluation of forages by near infrared reflectance spectroscopy

Since the potential of near infrared (NIR) spectroscopy for forage evaluation was discovered three decades ago, it has become clear that it is a powerful tool for the estimation of chemical components in these feed-stuffs. In addition, it has been successfully applied for the estimation of digestibility and degradability parameters, thus facilitating livestock ration formulation. The present review deals with the main reference methods that have been used in order to achieve proper calibration of NIR spectroscopy. Special attention will be focused on the weak points of these procedures with the intention of increasing NIR spectroscopy potential in this area.

Influence of harvest season, cutting frequency and nitrogen fertilization of mountain meadows on yield, floristic composition and protein content of herbage

The aim of the present study was to investigate the influence of cutting frequency, harvest season and nitrogen fertilization on yield and floristic composition of the herbage harvested from a mountain meadow. The meadow was divided into 22 plots, each receiving a different N P K fertilization treatment. The study lasted seven years, and during the first three years (1985-1987) each plot was harvested twice per year (June and September) according to a traditional harvest system, whereas in the years 1989-1991 each plot was harvested three times per year (spring, summer and autumn) following a more intensive harvest system. Nitrogen fertilizer favoured the development of the grasses in spring, thus reducing the proportion of legumes in the two cut harvest system, whereas grasses reached an advanced stage of maturity before the first cut in late June. However, the three cut harvest system entailed an earlier first cut in spring. As a result, the production of legume biomass was significantly increased both in the first cut and in the subsequent regrowths. No significant response in total herbage production to the N fertilizer was observed in the more intensive harvest system. Therefore, the three cut harvest system without nitrogen fertilization seemed to be the most suitable practice for the management of these botanically-complex mountain meadows. These results may contribute to design fertilization and management practices of mountain hay meadows to optimize their productivity and sustainability.

Prediction of aspects of neutral detergent fibre digestion of forages by chemical composition and near infrared reflectance spectroscopy

Sixty-two herbage samples, harvested in natural meadows located in the mountains of Leon (north-west Spain), and characterised by a diverse botanical composition and different stages of maturity of the plants, were used to evaluate the ability of chemical composition and near infrared (NIR) spectroscopy to predict in vitro digestibility and in sacco degradability of the neutral detergent fibre (NDF) fraction. In vitro digestibility was performed as described by the Goering and Van Soest procedure. Three dry Holstein-Friesian cows fitted with a rumen cannula were used to incubate the herbage samples. A Bran+Luebbe InfraAlyzer 500 spectrophotometer was used to obtain the NIR spectra corresponding to the 62 original herbage samples. Prediction equations for the estimation of in vitro digestibility and in sacco degradability parameters of the NDF fraction were generated using NIR spectra or chemical data as independent variables. The results showed that the in vitro digestibility and kinetic parameters of degradation of the NDF fraction could not be predicted accurately, probably as a consequence of the errors corresponding to the reference methods. In contrast, these errors did not greatly affect the extent of disappearance of the NDF fraction at later times, so the accuracy of prediction of these parameters was higher, especially when NIR spectra were used as independent variables. This is probably due to the close relationship that the parameters showed with the chemical data, since this kind of information, together with some physical characteristics of the samples, is included in the NIR spectra.

Nutritive value of herbage from mountain hay meadow managed under traditional and intensive harvest systems as affected by nitrogen fertilisation and time of cutting

The aim of this study was to investigate the influence of harvest season and nitrogen (N) fertilisation on chemical composition and digestibility of the herbage harvested from a mountain meadow (of the vegetation type Arrhenatheretalia). Four rates of N fertiliser (0, 60, 120 and 180 kg N/ha per year in a single spring application of calcium ammonium nitrate) were compared in field-replicated plots. The experiment lasted 7 years. During the first 3 years each plot was harvested twice per year (June and September) according to a traditional harvest system, whereas in the last 3 years (after a transitional year) each plot was harvested three times per year (spring, summer and autumn) following a more intensive harvest system. In both harvest systems, herbage collected in the first cut (early or late June) had higher fibre contents and lower digestibility (P 0.10) herbage yield in the 3-cuts system. N fertiliser changed the botanical composition of herbage and promoted grass growth, resulting in increased (P < 0.05) fibre content and decreased (P < 0.05) digestibility and rate of degradation of herbage, these effects being variable in the different harvest seasons. Our results suggest that the more intensive management system without N fertilisation could be considered a suitable practice for the management of these botanically complex mountain meadows. These results may contribute to design fertilisation and management practices of mountain hay meadows to optimise their productivity and sustainability.