Ali Kemal Uzunlar

Effect of Mobile Phone Exposure on Apoptotic Glial Cells and Status of Oxidative Stress in Rat Brain

The aim of this study was to investigate the effects of mobile phone exposure on glial cells in brain. The study carried out on 31 Wistar Albino adult male rats. The rat heads in a carousel exposed to 900 MHz microwave. For the study group (n:14), rats exposed to the radiation 2h per day (7 days in a week) for 10 months. For the sham group (n:7), rats were placed into the carousel and the same procedure was applied except that the generator was turned off. For the cage control (n:10), nothing applied to rats in this group. In this study, rats were euthanized after 10 months of exposure periods and brains were removed. Brain tissues were immunohistochemically stained for the active (cleaved) caspase-3, which is a well-known apoptosis marker, and p53. The expression of the proteins was evaluated by a semi-quantitative scoring system. However, total antioxidative capacity (TAC), catalase, total oxidant status (TOS), and oxidative stress index were measured in rat brain. Final score for apoptosis in the exposed group was significantly lower than the sham (p < 0.001) and the cage control groups (p < 0.01). p53 was not significantly changed by the exposure (p > 0.05). The total antioxidant capacity and catalase in the experimental group was found higher than that in the sham group (p < 0.001, p < 0.05). In terms of the TOS and oxidative stress index, there was no statistically significant difference between exposure and sham groups (p > 0.05). In conclusion, the final score for apoptosis, total antioxidant capacity and catalase in rat brain might be altered by 900 MHz radiation produced by a generator to represent exposure of global systems for mobile communication (GSM) cellular phones.

Diffuse lipomatosis of thyroid gland

A case of diffuse lipomatosis of the thyroid gland is presented. Previously documented cases of this rare disorder are reviewed. Diffuse lipomatosis of the thyroid, amyloid goiter with adipose tissue, and the relationship between lipomatosis and adenolipoma are discussed.

Effects of Melatonin Administration on Intestinal Adaptive Response After Massive Bowel Resection in Rats

This study evaluates whether melatonin can improve the structure of the small intestine and enhance adaptation in an experimental model of short bowel syndrome. Thirty Sprague–Dawley rats were divided randomly into three experimental groups of 10 animals each. In one group, only laparotomy was performed and these rats served as the sham-control group (G1). The remaining 20 rats underwent 90% small bowel resection (SBR) and formed the two experimental groups: the SBR/untreated group (G2), and the SBR/melatonin-treated group (G3). Rats in the SBR/untreated group received no therapeutic treatment. Rats in the SBR/melatonin-treated group received melatonin intraperitoneally for 3 weeks. The animals were weighed daily. All rats underwent relaparotomy on day 21 of the experiment. Remnant small bowel was excised and evaluated for villus height, total mucosal thickness, and crypt cell mitosis. After the 90% SBR, all animals suffered from diarrhea and weight loss between the first and the sixth postoperative days. The body weight of the SBR/melatonin group showed significant increases at the beginning of postoperative day 10 and day 21 in comparison to that of the SBR/untreated group. The rats treated with melatonin had significantly greater villus height and crypt cell mitosis compared to the sham-control group and the SBR/untreated group. In addition, the mucosal thickness was significantly increased in the SBR/melatonin-treated group compared to the SBR/untreated rats. These observations suggest that melatonin treatment increases villus height, total mucosal thickness, and crypt cell mitosis after massive SBR and it may exert a considerable effect on the mucosal adaptive response in short bowel syndrome in rats.

Mibefradil, a T-Type Ca2+ Channel Blocker, Protects Against Mesenteric Ischemia-Reperfusion-Induced Oxidative Injury and Histologic Alterations in Intestinal Mucosa in Rats

The purpose of the present study was to investigate whether mibefradil can reduce oxidative stress and histologic damage in the rat small bowel subjected to mesenteric ischemia and reperfusion injury. Thirty Sprague-Dawley rats weighing between 210 and 220 g were divided into three groups, each containing 10 rats: group 1, sham operation; group 2, untreated ischemia-reperfusion; and group 3, ischemia-reperfusion plus mibefradil treatment group. Intestinal ischemia for 45 min and reperfusion for 60 min were applied. Ileal specimens were obtained to determine the tissue levels of MDA, CAT, SOD, and GSH-Px and histologic changes. In group 2, MDA values were significantly increased compared to those in groups 1 and 3. In addition, SOD, CAT, and GSH-Px values decreased significantly in group 2 compared to groups 1 and 3. The intestinal injury score increased significantly in group 2 and 3 rats compared to group 1 rats. However, this increase was reduced in group 3 rats compared to group 2. Histopathologically, the rats in group 1 had essentially normal testicular architecture. In group 2 rats, the lesions varied between grade 3 and grade 5. In contrast, most of the specimens in the mibefradil-treated group 3 showed grade 1 injury. Mibefradil plays a role in attenuating reperfusion injury of the small intestine by depressing free radical production and mucosal injury score and regulating postischemic intestinal perfusion while restoring intestinal microcirculatory blood flow and encountered histologic injury.

Epidermoid cyst of the cecum in an elderly man

Ann Saudi Med 2006;26(6):477-479 Epidermoid cyst of the cecum is extremely rare. There have been only five reports of epidermoid cysts of the cecum in the English literature. The lesion has been reported in other internal organs in the epididymis, testis, spleen, accessory spleen and liver.1-9 I report the first case of epidermoid cyst of the cecum in the oldest patient reported thus far and discuss the origin of this entity.

Experimental study on effects of deferoxamine mesilate in ameliorating cisplatin-induced nephrotoxicity

Purpose: Cisplatin (CCDP), an indispensable agent of several chemotherapy protocols, has serious dose limiting side effects, including nephrotoxicity. In this experimental study, we used deferoxamine mesilate (DFO), an iron chelating agent, to ameliorate cisplatin-induced nephrotoxicity. Materials and methods: Sixty adult male bulb-c mice were divided in 6 equal groups. Group 1 received distilled water, group 2 received 100 mg/kg DFO, group 3 received 0.9 mg/kg CCDP, group 4 received 100 mg/kg DFO one hour before 0.9 mg/kg CCDP, group 5 received 1.8 mg/kg CCDP, and group 6 received 200 mg/kg DFO one hour before 1.8 mg/kg CCDP transperitoneally for 10 days. The next day, blood and urine samples were obtained, and all the animals were sacrificed, the kidneys and testes were removed, and histopathologic and biochemical analyses were performed. Results: Low-dose and high-dose CCDP treated mice had significantly more extensive proximal tubular degeneration (p < 0.001) when compared to control animals. Moreover, these changes were significantly less extensive in the mice taking DFO than mice taking CCDP. DFO showed no effect on cisplatin induced testicular histopathology. The cisplatin administration significantly increased the serum urea and plasma creatinin concentrations, and DFO administration prior to CCDP significantly decreased serum urea and plasma creatinin concentrations. Conclusion: Our findings suggest that DFO administration may be safe and useful for ameliorating cisplatin-induced nephrotoxicity.

Castleman's disease in the porta hepatis

Abstract. We report the CT, ultrasonographic, and pathologic features of a localized forms of Castlemans disease in the porta hepatis due to their unusual location. Our report suggests that the CT and the ultrasonographic features of Castlemans disease in these locations without calcifications is nonspecific.

PERSISTENT MüLLERIAN DUCT SYNDROME ASSOCIATED WITH TRANSVERSE TESTICULAR ECTOPIA: REPORT OF TWO CASES

Persistent Mullerian duct syndrome (PMDS) with transverse testicular ectopia (TTS) is a very rare pathological association, often discovered during repair for inguinal hernia or cryptorchidism. We present 2 cases with PMDS associated with TTS and discuss the diagnosis and management. A hysterectomy was performed, with resection of the underdeveloped fallopian tubes. Both gonads wereplaced into subdartos pouches in each scrotum by the transseptal approach. The diagnosis of PMDS was confirmed postoperatively by genetic and hormonal investigations. Chromosome analysis revealed 46,XY. Pathological findings demonstrated the immature testes and the immature uterus.

EFFECT OF EXTREMELY LOW FREQUENCY MAGNETIC FIELD AND MOBILE PHONE EXPOSURE ON NASAL MUCOSA AND NOSE SKIN

ABSTRACT The increasing usage of wireless comunication systems and electric-electronical systems in daily life has caused a concern over possible adverse effects of radio frequency radiation (RFR) and Extremely Low Frequency Magnetic Fields (ELF-MF) on biological systems. The aim of the study was to investigate the long-term effect of900 MHz RFR and ELF-MF exposure on nasal mucosa and nose skin of rats. In this study two different exposure systems were used to get information about the effects of RFR emitted from mobile phones and ELF-MF on nose. In the first part of the study, Twenty one Wistar Albino adult male rats (sham: 7, exposure: 14) were used to determine the effects of 900 MHz RFR on nose. For the exposure group, rat heads were exposed to the 900 MHz RFR 2 h per day (7 days in a week) for 10 months. For the sham group, same process was applied to the rats, except that the generator was turned off. In the second part of the study, 30 male Sprague-Dawley rats (sham: 10, first exposure: 10, second exposure: 10) were used to determine the effects of 100 μT and 500 μT extremely low frequency magnetic field (ELF-MF), which complies to the safety standards for public and occupational exposure. The rats in the exposure groups of this part of the study were exposed to 100 μT and 500 μT ELF-MF for 2 hours per day (7 days in a week) during 10 months in a Plexiglas cage. For the sham group, the same experimental process was applied to the rats but ELF-MF generator was turned off. The results of the present study showed that 10 months of exposures of mobile phones and ELF-MF do not affect the mucous membrane and skin of nose. In conclusion, radio frequency and ELF-MF used in this study do not affect nasal skin and mucosa of rats exposed to 900 MHz RFR, 100 μT and 500 μT ELF-MF.