Aliakbar Taherian

Effect of Diet Contains Sesame Seed on Adult Wistar Rat Testis

Estudios demuestran que algunos antioxidantes son eficaces en la mejora de la infertilidad masculina. Debido a la presencia de varios compuestos antioxidantes que existen en la semilla de sesamo, este estudio fue disenado y realizado para evaluar los efectos de su consumo sobre la estructura testicular y las hormonas sexuales de ratas macho adultas. Se utilizaron 30 ratas Wistar adultas de 200 g obtenidas desde el centro laboratorio animal de Kashan University of Medical Sciences. Las ratas se dividieron en grupos experimental y de control. El grupo control recibio una dieta estandar y el grupo experimental una dieta que contenia 70% de dieta estandar y 30% de semillas de sesamo, despues del destete durante 12 semanas. Al final del estudio, se midieron el peso y volumen de los testiculos y tubulos seminiferos, diametro luminal epitelial, y las concentraciones de LH, FSH y testosterona. Los datos fueron analizados mediante t-test con el programa SPSS. Fue considerado significativo un valor P<0,05. El peso corporal de las ratas, peso y volumen testicular, y el porcentaje volumetrico de los tubulos seminiferos en los dos grupos no fue significativo. La media del numero de celulas y la motilidad de los espermatozoides en epididimo izquierdo, numero de celulas del epitelio y porcentaje volumetrico del epitelio, y lumen intersticial de los tubulos fueron significativos (P<0,0001) en el grupo experimental en comparacion con el control. La concentracion de LH aumento significativamente en el grupo experimental en comparacion con el control (P<0,03). La ingesta de semillas de sesamo mejora de los parametros testiculares, la fertilidad y la produccion de espermatozoides en machos.

The Effects of Vasectomy on Epididymal Morphology and Sperm Parameters in Adult Male Balb/c mice

La vasectomia es un procedimiento anticonceptivo comun en los hombres. El presente estudio tuvo como objetivo explorar el impacto de la vasectomia sobre la morfologia del epididimo y los parametros espermaticos en ratones macho adultos Balb/c. Fueron utilizados en el estudio 20 ratones adultos (8 semanas de edad), con un peso ponderado de 20-30 g. Se dividieron en 2 grupos (vasectomia y Sham). Las cirugias se realizaron bajo anestesia con pentobarbital sodico (40 mg/kg de peso corporal, IP), con acceso a traves de una incision medio- abdominal inferior. La cabeza del epididimo izquierdo fue fijada para estudios histologicos y la cabeza del epididimo derecho se utilizo para el conteo de espermatozoides y evaluar su motilidad. La motilidad progresiva rapida y lenta de los espermatozoides se redujo significativamente en el grupo de ratones vasectomizados en comparacion con el grupo Sham (p<0,05), y el numero de espermatozoides inmoviles en el grupo sometido a la vasectomia aumento. Granuloma espermatico se observo en el 60% de los epididimos despues de la vasectomia. Tambien, el estudio histologico mostro un aumento del diametro del lumen tubular, espacio intersticial e infiltracion de celulas inmunitarias en el tejido intersticial en el grupo sometido a la vasectomia . La vasectomia aumenta los cambios histopatologicos en el epididimo y disminuye la motilidad de los espermatozoides, generando una reduccion en las tasas de fertilidad.

Wandering Spleen in an Adult Man Associated With the Horseshoe Kidney

Introduction A wandering spleen occurs when there is a laxity of the ligaments that fix the spleen in its normal anatomical position. Case Presentation This is a case report of a wandering spleen with horseshoe kidney in a 29-year-old male admitted with acute lower abdominal pain and vomiting to emergency department of Shariati hospital in Isfahan province. Sonographic examination showed a homogeneous 21 × 15 × 8 cm mass in the lower part of the abdomen and pelvis associated with a horseshoe kidney. Laparotomy confirmed the clinical and ultrasound findings. Conclusions The association of horseshoe kidney with a wandering spleen in this case may be due to an embryological anomaly.

The combination of retinoic acid and estrogen can increase germ cells genes expression in mouse embryonic stem cells derived primordial germ cells

Generation of germ cells from embryonic stem cells in vitro could have great application for treating infertility. The temporal expression profile of several genes was expressed at different stages of germ cell development and examined in differentiation the mouse embryonic stem cells. Cells were treated in three groups of control, with 10-8 M of all-trans retinoic acid and the combination of 10-9 M of 17β-Estradiol and retinoic acid for 7, 12, 17 or 22 days. Quantitative RT-PCR and Immunofluorescent were used to investigate the possible inductive effects of estrogen on mouse embryonic stem cell-derived primordial germ cells. mRNA expression of Oct4 and Dazl were downregulated in embryonic stem cells by the retinoic acid group, whereas Mvh transcription was reduced by retinoic acid and estrogen group in these cells compared to the control group. But, retinoic acid with estrogen group-treated cells exhibited increased mRNA expression of Stra8, Fragilis, Sycp3, GDF9, and Stella compared to untreated controls. The expression of Stella and Mvh proteins were remarkably increased in cell colonies. This study shows that estrogen affects the expression of specific markers of primordial germ cells. Also, estrogen and retinoic acid speed up and increase the level of expression of specific markers.

Comparing Apoptosis and Necrosis Effects of Arctium Lappa Root Extract and Doxorubicin on MCF7 and MDA-MB-231 Cell Lines

Objective: Breast cancer is a heterogeneous disease and very common malignancy in women worldwide. The efficacy of chemotherapy as an important part of breast cancer treatment is limited due to its side effects. While pharmaceutical companies are looking for better chemicals, research on traditional medicines that generally have fewer side effects is quite interesting. In this study, apoptosis and necrosis effect of Arctium lappa and doxorubicin was compared in MCF7, and MDA-MB-231 cell lines. Materials and Methods: MCF7 and MDA-MB-231 cells were cultured in RPMI 1640 containing 10% FBS and 100 U/ml penicillin/streptomycin. MTT assay and an annexin V/propidium iodide (AV/PI) kit were used respectively to compare the survival rate and apoptotic effects of different concentrations of doxorubicin and Arctium lappa root extract on MDA-MB-231 and MCF7 cells. Results: Arctium lappa root extract was able to reduce cell viability of the two cell lines in a dose and time dependent manner similar to doxorubicin. Flow cytometry results showed that similar to doxorubicin, Arctium Lappa root extract had a dose and time dependent apoptosis effect on both cell lines. 10µg/mL of Arctium lappa root extract and 5 µM of doxorubicin showed the highest anti-proliferative and apoptosis effect in MCF7 and MDA231 cells. Conclusion: The MCF7 (ER/PR-) and MDA-MB-231 (ER/PR+) cell lines represent two major breast cancer subtypes. The similar anti-proliferative and apoptotic effects of Arctium lappa root extract and doxorubicin (which is a conventional chemotherapy drug) on two different breast cancer cell lines strongly suggests its anticancer effects and further studies.