Alice K.Y. Chan

Ethoxyresorufin-O-deethylase enzyme activities and accumulation of secondary/tertiary lysosomes in rabbitfish Siganus oramin as biomarkers for xenobiotic exposures

The sensitivities of using hepatic and intestinal ethoxyresorufin-O-deethylase (EROD) activities and hepatic accumulation of secondary/tertiary (2 degrees/3 degrees) lysosomes to detect xenobiotic exposures were assessed in the rabbitfish Siganus oramin in a metropolitan harbour, subtropical Hong Kong, over a complete seasonal cycle of one year. Additional information on the body-burden pollutants and physiological indices in S. oramin, and seasonal variables in seawater quality, were extracted from published data and re-analyzed. Under the influences of pollutant cocktail and seasonal factors, neither the hepatic nor intestinal EROD activity was indicative of total polycyclic aromatic hydrocarbons (Sigma PAH), total polychlorinated biphenyls, condition factor and hepatosomatic index (HSI) in S. oramin. However, the relative ratio of hepatic to intestinal EROD activities provided an indication to differentiate the xenobiotic intake route in the fish through diffusion via gills/skin or consumption of contaminated food. In addition, the elevated hepatic accumulation of 2 degrees/3 degrees lysosomes was closely associated with the dominant temporal trends of zinc and Sigma PAH, as well as reduced HSI, in S. oramin. Being minimally influenced by any investigated seasonal factors, the hepatic 2 degrees/3 degrees lysosomes in S. oramin was recommended as an effective biomarker of xenobiotic exposures and toxic effects for use in coastal pollution monitoring programmes.

Sediment characteristics and benthic ecological status in contrasting marine environments of subtropical Hong Kong.

Sediment characteristics and benthic communities on a finer sampling scale in four contrasting environments in subtropical Hong Kong were analyzed in summer and winter 2012. In two harbour habitats which suffered from historic sewage pollution or hypoxic events, organic carbon, nutrient and trace metal content in the sediment were significantly higher than that in an offshore area and a marine reserve. The relatively low organic and nutrient content in the offshore habitat could be resulted from enhanced resuspension of such materials from the seabed owing to intense water mixing and disturbance caused by bottom trawling. The biotic indices AMBI and M-AMBI were shown to be useful in assessing the benthic ecological status of these habitats. Such indices can also be more sensitive than sediment physico-chemical parameters in differentiating the response of macrofauna to seasonal changes in the benthic environment.

Susceptibility of fish to Chattonella marina is determined by its tolerance to hypoxia.

The harmful alga Chattonella marina has caused massive fish kills and economic losses worldwide. However, the fish kill mechanisms by C. marina have not been identified. The present study has confirmed that a significant elevation of blood osmolality is the universal response in moribund fish exposed to C. marina and the possible reasons leading to contradictory reports were identified. Both osmotic distress and respiratory impairment are important mechanisms leading to fish kill by C. marina. The susceptibility of marine fish to C. marina appears to be inversely related to their tolerance to hypoxia, with the hypoxia intolerant goldlined seabream being the most susceptible, and the hypoxia tolerant green grouper being the most tolerant to C. marina. Further studies in the marine medaka (Oryzias melastigma) showed that fish susceptibility to C. marina is directly related to susceptibility of the fish to hypoxia, but not related to its tolerance to hypersalinity stress.

Trophic and growth baseline of dominant subtidal gastropods in contrasting subtropical marine environments

Using 13C/12C, 15N/14N and 18O/16O isotopes, the trophic relationship and growth estimation were analyzed in gastropods Nassarius siquijorensis, Murex trapa and Turritella bacillum and their potential food sources and predators in summer and winter from estuarine and oceanic environments in subtropical Hong Kong. Results of δ13C and δ15N values and isotopic mixing model revealed N. siquijorensis and M. trapa were one trophic level higher than T. bacillum, in which its main food source was particulate organic matter (POM) whereas N. siquijorensis largely consumed POM and polychaetes and M. trapa also preyed on other gastropods. Crabs were the major predator of gastropods. Organisms collected from oceanic waters were more 13C enriched than from estuarine waters, reflecting different carbon food sources from marine or terrestrial origin. The δ18O profile from shell carbonate suggested these gastropods were one to two years old. T. bacillum exhibited faster summer growth than the other two species.