Alicia Snider

Abstract 69: Stem Cells Harvested from Bone Marrow and Adipose Tissue Demonstrate Equivalent Healing but Through Different Mechanisms in a Murine Model of Irradiated Mandibular Fracture Healing

METHODS: Immunocompromised nude-mice underwent external beam irradiation of the scalp. Five weeks later, mice either received seven deferoxamine treatments (1mg in 100ul) or saline subcutaneously to the irradiated area every other day. Laser Doppler analysis (LDA) was recorded prior to irradiation, following irradiation, and 24 hours following each treatment. Human fat grafts were then injected in the subcutaneous plane of the scalp and volume retention measured by CT scan over 8 weeks. Finally, skin and fat samples were evaluated histologically for vasculature, dermal thickness, and fat graft quality.

Abstract 48. Stem Cells Harvested from Bone Marrow and Adipose Tissue Demonstrate Differential Healing Mechanisms and Union Rates in a Murine Model of Irradiated Mandibular Fracture Repair

METHODS: Custom 3DBC scaffolds were either coated with collagen (control) or coated with collagen and immersed in 100 μM dipyridamole (DIPY). Sheep (n=5) were subjected to 4 trephine-induced (12 mm diameter) calvarial defects with immediate scaffold placement via two separate operations: anteriorly (control) and posteriorly (DIPY) on the right (3 weeks healing) and left (6 weeks healing) sides of the calvarium. Following sacrifice, defects were evaluated through microcomputed tomography and histologic analysis for bone, scaffold, and soft tissue quantification as a function of time in vivo. Statistical analysis was performed by a mixed model and significance was defined by p<0.05.

Remediation of Radiation-Associated Vasculopathy Utilizing a Novel Angiogenic Nanotechnology

RESULTS: 4 cm long full-thickness skin wounds were incised on the back of mice. The mice were locally treated by subcutaneous injections of CD24 derivatives from either bacterial or mammalian cell sources and compared to untreated mice. Wounds were histologically analyzed and scored, based on the degree of cellular invasion, granulation tissue formation, vascularity, and re-epithelialization. In the treated mice the wounds closed significantly faster then the untreated mice. No statistically significant difference was seen between bacterial and mammalian origin derivatives.

Abstract: Stem Cells Harvested from Bone Marrow and Adipose Tissue Demonstrate Equivalent Healing Potential in a Murine Model of Irradiated Mandibular Fracture Healing

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