Aline Desmedt
Centre national de la recherche scientifique
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Featured researches published by Aline Desmedt.
Nature Neuroscience | 2005
Jean-Michel Revest; Francesco Di Blasi; Pierre Kitchener; Françoise Rougé-Pont; Aline Desmedt; Marc Turiault; François Tronche; Pier Vincenzo Piazza
Many of the behavioral consequences of stress are mediated by the activation of the glucocorticoid receptor by stress-induced high levels of glucocorticoid hormones. To explore the molecular mechanisms of these effects, we combined in vivo and in vitro approaches. We analyzed mice carrying a brain-specific mutation (GRNesCre) in the glucocorticoid receptor gene (GR, also called Nr3c1) and cell lines that either express endogenous glucocorticoid receptor or carry a constitutively active form of the receptor (ΔGR) that can be transiently induced. In the hippocampus of the mutant mice after stress, as well as in the cell lines, activation of glucocorticoid receptors greatly increased the expression and enzymatic activity of proteins in the MAPK signaling pathway and led to an increase in the levels of both Egr-1 mRNA and protein. In parallel, inhibition of the MAPK pathway within the hippocampus abolished the increase in contextual fear conditioning induced by glucocorticoids. The present results provide a molecular mechanism for the stress-related effects of glucocorticoids on fear memories.
The Journal of Neuroscience | 1998
Aline Desmedt; René Garcia; Robert Jaffard
Recent data obtained using a classic fear conditioning paradigm showed a dissociation between the retention of associations relative to contextual information (dependent on the hippocampal formation) and the retention of elemental associations (dependent on the amygdala). Furthermore, it was reported that conditioned emotional responses (CERs) could be dissociated from the recollection of the learning experience (declarative memory) in humans and from modifications of the hippocampal–septal excitability in animals. Our aim was to determine whether these two systems (“behavioral expression” system and “factual memory” system) interact by examining the consequences of amygdalar lesions (1) on the modifications of hippocampal–septal excitability and (2) on the behavioral expression of fear (freezing) resulting from an aversive conditioning during reexposure to conditional stimuli (CSs). During conditioning, to modulate the predictive nature of the context and of a discrete stimulus (tone) on the unconditional stimulus (US) occurrence, the phasic discrete CS was paired with the US or randomly distributed with regard to the US. After the lesion, the CER was dramatically reduced during reexposure to the CSs, whatever the type of acquisition. However, the changes in hippocampal–septal excitability persisted but were altered. For controls, a decrease in septal excitability was observed during reexposure to the conditioning context only for the “unpaired group” (predictive context case). Conversely, among lesioned subjects this decrease was observed in the “paired group” (predictive discrete CS case), whereas this decrease was significantly reduced in the unpaired group with respect to the matched control group. The amplitude and the direction of these modifications suggest a differential modulation of hippocampal–septal excitability by the amygdala to amplify the contribution of the more predictive association signaling the occurrence of the aversive event.
The Journal of Neuroscience | 2006
Ludovic Calandreau; Pierre Trifilieff; Nicole Mons; Laurence Costes; Marc Marien; Jacques Micheau; Robert Jaffard; Aline Desmedt
Ample data indicate that tone and contextual fear conditioning differentially require the amygdala and the hippocampus. However, mechanisms subserving the adaptive selection among environmental stimuli (discrete tone vs context) of those that best predict an aversive event are still elusive. Because the hippocampal cholinergic neurotransmission is thought to play a critical role in the coordination between different memory systems leading to the selection of appropriate behavioral strategies, we hypothesized that this cholinergic signal may control the competing acquisition of amygdala-mediated tone and contextual conditioning. Using pavlovian fear conditioning in mice, we first show a higher level of hippocampal acetylcholine release and a specific pattern of extracellular signal-regulated kinase 1/2 (ERK1/2) activation within the lateral (LA) and basolateral (BLA) amygdala under conditions in which the context is a better predictor than a discrete tone stimulus. Second, we demonstrate that levels of hippocampal cholinergic neurotransmission are causally related to the patterns of ERK1/2 activation in amygdala nuclei and actually determine the selection among the context or the simple tone the stimulus that best predicts the aversive event. Specifically, decreasing the hippocampal cholinergic signal not only impaired contextual conditioning but also mimicked conditioning to the discrete tone, both in terms of the behavioral outcome and the LA/BLA ERK1/2 activation pattern. Conversely, increasing this cholinergic signal not only disrupted tone conditioning but also promoted contextual fear conditioning. Hence, these findings highlight that hippocampal cholinergic neurotransmission controls amygdala function, thereby leading to the selection of relevant emotional information.
European Journal of Neuroscience | 1999
Aline Desmedt; René Garcia; Robert Jaffard
Previous experiments using a classical fear conditioning paradigm have provided evidence that the processing of contextual conditional stimuli (CSs) by the hippocampus would be controlled by the amygdala through a modulation of hippocampal‐lateral septal (H‐LS) excitability. More specifically, our suggestion was that vasopressin release into the LS would occur in an elemental conditioning case [pairing CS–US (unconditional stimulus) procedure] and would result in less hippocampal‐dependent contextual stimuli processing (i.e. overshadowing of CSs by the simple CS). Conversely, when an unpairing CS–US procedure is used, this would result in more contextual stimuli processing through a decrease in vasopressin release into the LS. The aim of the present experiment was to test this hypothesis using intraseptal injection of vasopressin or its V1/V2 antagonist. In agreement with this hypothesis, results suggest that vasopressin release into the LS would constitute a device by which priority is given to the more salient simple stimulus to the detriment of contextual information.
European Journal of Neuroscience | 2003
Aline Desmedt; Aline Marighetto; René Garcia; Robert Jaffard
To what extent the hippocampus is required for contextual conditioning remains a matter of debate. The present experiments examined the effects of ibotenate hippocampal lesions on discriminative fear conditioning to context in mice using measures of freezing in two conditioning paradigms. In both paradigms animals received foot shock as the unconditional stimulus (US) when placed in the (conditioning) context and no foot‐shock when placed in the other (neutral) context. In both contexts, animals were presented with a tone as the conditioned stimulus (CS). In the conditioning context there was either no interval (delay condition) or a 30‐s interval (trace condition) between tone CS end and shock US onset. These two paradigms were used because theory predicts that in the trace condition animals would learn more about contextual cues as predictors, or not, of shock US occurrence than in the delay condition. In agreement with this, we observed that sham‐operated mice learned the context discrimination faster in the trace than in the delay condition. Lesions of the hippocampus significantly retarded, but did not prevent, the acquisition of the context discrimination in the trace condition. In contrast, lesions produced an opposite (facilitatory) effect in the delay condition, which was mainly observed during tone CS presentation. The data suggest that mice used two distinct competing strategies in solving this discrimination task: (i) a strategy relying on the processing of background contextual stimuli allowing direct establishment of context–US associations of different strengths, and (ii) a conditional cue (tone)‐based strategy. Hence, hippocampal lesions may impair the use of the former strategy while exacerbating (unmasking) the use of the latter.
Learning & Memory | 2010
Ludovic Calandreau; Bertrand Desgranges; Robert Jaffard; Aline Desmedt
The aim of the present experiment was to directly assess the role of the glutamatergic hippocampal-lateral septal (HPC-LS) neurotransmission in tone and contextual fear conditioning. We found that pretraining infusion of glutamatergic acid into the lateral septum promotes tone conditioning and concomitantly disrupts contextual conditioning. Infusion of glutamatergic antagonist, on the contrary, promotes contextual conditioning to the detriment of tone fear conditioning. These findings highlight the direct contribution of the glutamatergic HPC-LS neurotransmission to the adaptive selection among environmental stimuli of those that best predict the occurrence of the aversive event.
Learning & Memory | 2006
Pierre Trifilieff; Cyril Herry; Peter Vanhoutte; Jocelyne Caboche; Aline Desmedt; Gernot Riedel; Nicole Mons; Jacques Micheau
Learning & Memory | 2005
Ludovic Calandreau; Aline Desmedt; Laurence Decorte; Robert Jaffard
Brain Research Reviews | 2004
Dan Yaniv; Aline Desmedt; Robert Jaffard; Gal Richter-Levin
Learning & Memory | 2007
Ludovic Calandreau; Robert Jaffard; Aline Desmedt