Allan Leck Jensen
Aarhus University
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Publication
Featured researches published by Allan Leck Jensen.
Gene | 1995
Torben Heick Jensen; Allan Leck Jensen; Jørgen Kjems
We have constructed two new vectors for the production of foreign proteins in Escherichia coli. The vectors, pGEX-GTH and pET-HTG, produce protein fused to glutathione S-transferase (GST) at the N- and C-termini, respectively, allowing one-step purification on glutathione-Sepharose. Furthermore, they carry the recognition sequence (RRASV) for the catalytic subunit of cAMP-dependent heart muscle kinase (HMK) at the terminus distal to the GST tag, enabling specific 32P labeling in vitro. By positioning the GST and HMK sequences at opposite ends of the introduced gene, only full-length fusion protein becomes radiolabeled after purification. Avoiding the labeling of shorter fusion protein species, often observed in bacterial expression of foreign genes, is particularly important for a number of different purposes, including protein mobility shift analysis and protein footprinting technology.
FEBS Letters | 1997
Torben Heick Jensen; Allan Leck Jensen; Anne Marie Szilvay; Jørgen Kjems
Human immunodeficiency virus type 1 (HIV‐1) Rev is a small RNA‐binding protein which is essential for viral replication. To investigate the structure of Rev we have mapped the binding sites of a panel of monoclonal antibodies (mAb) by protein footprinting and identified a mAb protecting amino acids within both the N‐ and C‐terminal parts of Rev. Our mapping results support a previously proposed structure (Auer et al., Biochemistry, 33 (1994) 2988–2996) predicting that a helix–loop–helix motif in Rev brings the termini of the protein into proximity. Furthermore, we demonstrate that the binding sites mapped by protein footprinting are in agreement with conventional epitope mapping results and that this technique provides an advantageous strategy for mapping discontinuous sites.
Agricultural Systems | 2007
Nina K. Detlefsen; Allan Leck Jensen
Agricultural Systems | 2004
Nina K. Detlefsen; Allan Leck Jensen
Computers and Electronics in Agriculture | 2013
Ibrahim A. Hameed; Dionysis Bochtis; Claus G. Sørensen; Allan Leck Jensen; R. Larsen
Journal of Molecular Biology | 1998
Allan Leck Jensen; Torben Heick Jensen; Jørgen Kjems
Archive | 2017
Ilse A. Rasmussen; Allan Leck Jensen; Margit Styrbæk Jørgensen; Helene Kristensen; Dennis Christensen
IFOAM Organic World Congress 2017 | 2017
Ilse A. Rasmussen; Allan Leck Jensen; Margit Styrbæk Jørgensen; Helene Kristensen; Malgorzata Conder; Cristina Micheloni; Bram Moeskops
Archive | 2016
Ilse A. Rasmussen; Allan Leck Jensen
Spanish Journal of Agricultural Research | 2015
K. Zhou; Allan Leck Jensen; Dionysis Bochtis; C.G. Sørensen