Allison Jean Tanner

Closed system cell culture protocol using HYPERStack vessels with gas permeable material technology.

Large volume adherent cell culture is currently standardized on stacked plate cell growth products when microcarrier beads are not an optimal choice. HYPERStack vessels allow closed system scale up from the current stacked plate products and delivers >2.5X more cells in the same volumetric footprint. The HYPERStack vessels function via gas permeable material which allows gas exchange to occur, therefore eliminating the need for internal headspace within a vessel. The elimination of headspace allows the compartment where cell growth occurs to be minimized to reduce space, allowing more layers of cell growth surface area within the same volumetric footprint. For many applications such as cell therapy or vaccine production, a closed system is required for cell growth and harvesting. The HYPERStack vessel allows cell and reagent addition and removal via tubing from media bags or other methods. This protocol will explain the technology behind the gas permeable material used in the HYPERStack vessels, gas diffusion results to meet the metabolic needs of cells, closed system cell growth protocols, and various harvesting methods.

Novel Surface Technologies for Genomics, Proteomics, and Drug Discovery

Following the recent progress in functional genomics and proteomics, and high-throughput screening (HTS) in drug discovery, evolving technologies over the last decade have offered a tremendous leap over the caveats of traditional techniques. In response to this metamorphosis of technologies through different platforms, Corning has introduced a suite of surface technologies with applications in microarray printing, enhanced attachment, and consumables in drug discovery. Microarrays generated on an ultra-flat glass substrate with GAPS coating exhibiting a robust chemistry and low surface background have led to higher sensitivity and reproducibility for the expression assay. Recent introduction of UltraGAPS™ surface enables oligo attachment for use in differential gene expression analysis. Various attachment surfaces to meet the needs of the applications in genomics, proteomics and drug discovery will be discussed.