Almir R. Pepato

Reassessment of the evolutionary relationships within the dog‐faced bats, genus Cynomops (Chiroptera: Molossidae)

The low representativeness of the dog‐faced bats (genus Cynomops Thomas, ) in collections has constrained the study of the diversity and the evolutionary relationships within this genus. Taxonomic revisions of some taxa, in particular the large‐sized Cynomops abrasus (Temminck, ), are crucial for understanding the phylogeny of Cynomops. A total of four subspecies of C. abrasus have been described to date, all widespread in South America: C. a. mastivus (Thomas, ), C. a. brachymeles (Peters, 1865), C. a. cerastes (Thomas, ) and C. a. abrasus (Temminck, ). Here, we evaluated the phylogenetic relationships within Cynomops, and the status of the four C. abrasus subspecies using complete sequences of two mitochondrial genes (Cyt b and COI) and 39 morphological characters. Maximum‐parsimony, maximum‐likelihood and Bayesian phylogenetic reconstructions recovered a novel hypothesis for Cynomops, supported the recognition of C. a. mastivus as a distinct species, separated from C. abrasus, and two hypotheses of lineages previously unrecognized for Cynomops. The use of mitochondrial genes combined with morphological characters revealed again to be a powerful tool to recover the phylogenetic relationships within Cynomops and demonstrated that the genus is more diverse than previously thought.

New records of the genus Limnohalacarus (Halacaridae, Trombidiformes) from southern Brazil

Two Limnohalacarus species are reported from the southern Brazilian state of Rio Grande do Sul, Limnohalacarus cultellatus Viets, 1940 and L. mamillatus Fain & Lambrechts, 1987, originally described on the basis of individuals from the Caribbean region and aquaria in Belgium, respectively. The former species is also known from Madagascar, Hungary, North America, and was referred to Brazil and El Salvador in the literature. The latter species is also known from Western Australia.

The complex evolutionary history and phylogeography of Caridina typus (Crustacea: Decapoda): long-distance dispersal and cryptic allopatric species

The evolutionary history of the old, diverse freshwater shrimp genus Caridina is still poorly understood, despite its vast distribution – from Africa to Polynesia. Here, we used nuclear and mitochondrial DNA to infer the phylogeographic and evolutionary history of C. typus, which is one of only four species distributed across the entire range of the genus. Despite this species’ potential for high levels of gene flow, questions have been raised regarding its phylogeographic structure and taxonomic status. We identified three distinct lineages that likely diverged in the Miocene. Molecular dating and ancestral range reconstructions are congruent with C. typus’ early dispersal to Africa, possibly mediated by the Miocene Indian Ocean Equatorial Jet, followed by back dispersal to Australasia after the Jet’s closure. Furthermore, several different species delimitation methods indicate each lineage represents a distinct (cryptic) species, contradicting current morphospecies delimitation of a single C. typus taxon. The evolutionary history of C. typus lineages is complex, in which ancient oceanic current systems and (currently unrecognised) speciation events preceded secondary sympatry of these cryptic species.

Molecular phylogeny of marine mites (Acariformes: Halacaridae), the oldest radiation of extant secondarily marine animals

The family Halacaridae comprises more than one thousand mostly marine or rarely freshwater species. Many are predacious, but among marine mites, some genera evolved the ability to feed on macroalgae. We inferred a time-calibrated phylogeny based on 18S rDNA, 28S rDNA, and Cytochrome oxidase I (5,143 nt aligned) and all non-monotypic halacarid subfamilies plus a representative outgroup set (72 taxa). The family Halacaridae was rendered as the sister-group of Parasitengona, diverging 321.5, 264.0-381.3 Ma and radiating 271.3, 221.7-324.2 Ma (median, HPD). Thus, marine mites represent the oldest known extant animal lineage that secondarily invaded the sea, with the marine turtles being the second oldest such lineage (crown group 212.3, 194.9-231.4 Ma). Two freshwater mite lineages, represented by Limnohalacarus (219.2, 165.9-274.6) and Porohalacarus (175.3, 118.5-233.1), were inferred mutually non-monophyletic, suggesting two independent invasions to freshwater. The conventional subfamily Rhombognathinae (macroalgae feeders) was not recovered as monophyletic, with Metarhombognathus-Rhombognathides, restricted to the Northern Hemisphere, originating 177.5, 134.8-223.3 Ma and diversifying 88.3, 32.7-152.3 Ma. This is congruent to a previous hypothesis of their northern origin prior to the opening of the Norwegian Sea (58 Ma). Our phylogeny indicates the need for reclassification of the traditional subfamilies and suggests that previous molecular results (e.g., Rhombognathus deeply nested in Copidognathinae) is an analytical artifact due to a chimeric sequence.