Altero Aguzzi

Commercial mushrooms: nutritional quality and effect of cooking

With the aim of extending knowledge on chemical composition of mushrooms, commercial samples, widely consumed in Italy (Boletus group, Agrocybe aegerita and Pleurotus eryngii) have been examined to determine proximate composition and some components of nutritional interest such as dietary fibre, chitin, beta glucan and total phenols in raw and cooked samples. The moisture values ranged from 67.2 to 91.5 g/100 g edible weight in the raw samples, and from 71.9 to 90.4 g /100 g edible weight in the cooked samples. The lipid fraction was not relevant. Proteins, higher in Boletus group dried samples than in the other mushroom species (Agrocybe aegerita and Pleurotus eryngii), ranged from 1.5 to 7.9 g/100 g edible weight, while the ash content varied between 0.5 and 2.0 g/100 g of edible weight. Dried and re-hydrated Boletus samples showed higher levels of soluble and insoluble dietary fibre than the other samples. The amount of beta glucans varied within the same species (Boletus group samples) and represented from 2 to 13% of total dietary fibre. The chitin content ranged from 0.5 to 3.3 g/100 g edible weight. Finally, total phenols varied widely among different mushroom species, from 51.4 to 403.8 mg/100 g edible weight and from 70.4 to 301.6 mg/100 g edible weight, respectively, for raw and cooked samples.

Determination of folate vitamers in food and in Italian reference diet by high-performance liquid chromatography☆

A trienzyme treatment (conjugase, alpha-amylase, protease) followed by affinity chromatography and reversed-phase HPLC with UV and fluorescence detection was performed for the quantification of folate vitamers in legumes (chickpea and beans), processed meats (salami Milano and Parma ham) and in an Italian reference diet. This method allowed a good separation of six folate vitamers: 5-methyltetrahydrofolate, 5-formyltetrahydrofolate, folic acid, 10-formylfolic acid, 10-formyldihydrofolate and tetrahydrofolate within 30 min. Recovery, reproducibility and limits of detection of the method are reported. HPLC results were 24-52% lower than the microbiological assay findings.

Optimization of heme iron analysis in raw and cooked red meat

Abstract Optimization of analytical conditions for heme iron extraction and its quantitative determination in raw and cooked red meat was performed by Box-Hunter design, Response Surface Methodology (RSM) was used to find a relationship between factors and response. Six analytical parameters, percent HCl in extracting solution, sample weight, stirring time, developing color time, manual stirring time, centrifugation time, were assumed to be critical factors affecting heme iron extraction. HCl concentration (X 1 ), sample weight (X 2 ), and their interaction, was the main factors affecting the effectiveness of heme extraction from meat. Optimum conditions for maximizing heme extraction were for raw meat when X 1 was 0.38 M and X 2 was 1.54 g, for cooked meat when X 1 was 0.31 M and X 2 was 1.14 g. Beef meat analyzed in this study was characterized by a high total iron content and by a corresponding high level of heme iron, representing 86% of total iron content for raw meat and 83% for cooked meat.

Intake of vitamin A and carotenoids from the Italian population--results of an Italian total diet study.

The present study focused on vitamin A and carotenoids (alpha-and beta-carotene, lutein and zeaxanthin, beta-cryptoxanthin, lycopene) daily intake from the Italian total diet. The input of some food groups (cereals, vegetables, fruits, milk and dairy, meat and meat products, fish) most responsible for major and minor contributions to the daily intake of these molecules was evaluated. Furthermore the contribution to the dietary intake of beta-carotene and lutein of the most consumed vegetables in the market basket of the Italian total diet (beets, brassica vegetables, carrots, chicory, courgette (zucchini), green beans, lettuce, peas, pepper, spinach, tomatoes) was also investigated. Vitamin A daily intake was 855 mg/person/day. The vegetables food group made the greatest contribution (37%), followed by the meat and meat products food group (23%). The Italian total diet provided 14.3 mg/person/day of carotenoids; lycopene was the highest (7.4 mg/day), followed by lutein + zeaxanthin (4 mg/day), beta-carotene (2.6 mg/day), alpha-carotene (0.15 mg/day), and beta-cryptoxanthin (0.17 mg/day). Carrots and tomatoes were the main sources of beta-carotene in the diet, otherwise the daily consumption of leafy vegetables (spinach, beets, lettuce) made the main contribution to lutein + zeaxanthin daily intake.

Micronutrients in Italian ham: A survey of traditional products

The study provides original analytical data on the micronutrient profile of some traditional Italian hams, representative of the major ham categories produced in Italy: 4 dry-cured hams (Modena, Nazionale, Parma, San Daniele), 3 cooked hams (Cotto, Scelto, Alta Qualità), 1 smoked ham (Speck). Data on macronutrients (protein, lipid, moisture), energy, trace elements (Fe, Zn, Cu, Mn, Se), B vitamins (B1, B2, PP, B6, B12) and vitamin E level in the 80 Italian hams sampled are reported. Smoked and dry-cured ham were the richest sources of Fe, Zn and Se and, among vitamins, dry-cured ham had the highest level of B2, PP, B6 and B12; cooked ham provided the lowest energy intake. The contribution of ham to micronutrients recommended dietary allowances was estimated: a ham portion (50g) was a good source especially of Zn and Se providing over 12% of RDA of both; among B vitamins, dry-cured ham greatly contributed to B1 and B6 vitamins RDA (both over 30%).

Globulins enhance in vitro iron but not zinc dialysability: a study on six legume species.

The study was addressed to evaluate the in vitro iron and zinc dialysability from the globulin fraction of six legumes. Five legume species including white bean, mottled bean (Taylor bean), chickpea, lentil, lupin, and a modified mottled bean variety, selected by back-crossing to obtain seeds with globulins composed by G1 fraction only, were used. Globulins (G1 + G2) were extracted from the seeds and analysed for their in vitro iron and zinc dialysability. The highest globulin concentration was detected in lentil (89%). The percentage of globulins in the modified variety of Taylor bean (G1 only) was higher than that of the commercial variety (G1 + G2). The highest concentration of iron was found in Taylor bean globulins. The modified variety of Taylor bean contained 2.6-fold higher iron concentration than the whole seed, and the commercial variety had 1.8-fold higher iron only. The highest zinc concentration was found in lentil globulins. Also iron dialysability from globulins was markedly higher than that of the respective whole seed. The highest value of iron dialysability was found in lentil (10.8%). Zinc dialysability was generally high (above 20%), but no significant differences between whole seed and globulins were detected. The results showed that globulins enhanced iron but not zinc dialysability. Lupin and the modified variety of Taylor bean showed a different behaviour in terms of mineral dialysability compared to the other legumes. The amino acid composition of the digestion products of whole seeds and globulins failed to evidence any direct influence on iron and zinc availability.

Nutritional composition and antioxidant properties of traditional Italian dishes.

This work aims at evaluating the main nutrients (proximate, minerals), their contribution for Dietary Reference Values in Italian population, and the antioxidant properties of some popular dishes prepared in a dedicated lab-kitchen: spaghetti alle vongole, pomodori al riso, gateau di patate, carciofi alla romana, pan di Spagna. Results showed that nutrient contents and energy value varied extensively because of the variety and the nature of ingredients in the analyzed dishes, and thus defined different daily nutrient intakes and their association with health effects. A serving portion of either spaghetti alle vongole or carciofi alla romana showed to contribute to the daily nutritional recommendations respectively, with an appreciable percentage of available carbohydrates (30-22%) and a considerable content of dietary fibre (91%). Gateau di patate was particularly rich in calcium (343mg), both contributing 34% of the recommended intake, in addition to appreciable content of phosphorus and zinc. With regards to spaghetti alle vongole, pomodori al riso, gateau di patate and pan di Spagna, extractable polyphenols contributed less than 15% to total antioxidant activity, and hydrolysable polyphenols give a major contribution; carciofi alla romana showed an inverse trend. Findings provide an important contribution to the update of Italian Food Composition Databases.

Food Composition Databases: Considerations about Complex Food Matrices

Nowadays, many countries have their own national Food Composition Databases, whose continuous updating allows the inclusion of a large number of foods, reflecting the food habits of the population and the growing number of foods on the market in the best way possible. Therefore, particular attention should be directed to the study not only of individual foods or food components but also of the nutritional characteristics of dishes, meals and diets, as they are really consumed. Recently, a reviewed sensitivity in Europe towards the implementation of standardized procedures for generating reliable composition data for composite dishes has been carried out. Although direct chemical analysis is the most accurate method to determine food composition, the nutrient content of complex matrices and composite dishes is often calculated from the nutrient contents of the individual ingredients, considering the different thermal treatments and using some preparation factors. In this context, this paper aims to give an updated picture on Food Composition Databases; in particular, their application regarding complex matrices is examined together with the need to optimize their calculated nutritional values. Results obtained by this calculation should almost always be observed as approximations.

From Plant Compounds to Botanicals and Back: A Current Snapshot

This work aims at giving an updated picture of the strict interaction between main plant biologically active compounds and botanicals. The main features of the emerging class of dietary supplements, the botanicals, are highlighted. Focus is also on the definition of actual possibilities of study approach and research strategies. Examples of innovative directions are given: assessment of interaction of bioactive compounds, chemometrics and the new goal of biorefineries. Current models of existing databases, such as plant metabolic pathways, food composition, bioactive compounds, dietary supplements, and dietary markers, are described as usable tools for health research. The need for categorization of botanicals as well as for the implementation of specific and dedicated databases emerged, based on both analytical data and collected data taken from literature throughout a harmonized and standardized approach for the evaluation of an adequate dietary intake.