Alvaro Delgadillo

Extraction Techniques for Bioactive Compounds and Antioxidant Capacity Determination of Chilean Papaya (Vasconcellea pubescens) Fruit

The aim of this work was to assess and compare different extraction methods by using high hydrostatic pressure (HHPE), ultrasound (UE), agitation (AE), and their combinations for the extraction of bioactive compounds of Chilean papaya. Extract antioxidant capacity was evaluated by three methods (i.e., DPPH, FRAP, and Voltammetry) and phenolic compounds and vitamin C were determined by HPLC. Papaya sample extraction was performed by HHPE at 500 MPa for 10 min and UE and AE for 30 min, respectively. The combined-extractions: HHPE-UE and HHPE-AE, were carried out for 5 min and 15 min, respectively. The highest values found were total phenolic 129.1 mg GAE/100 g FW, antioxidant capacity by DPPH 20.6 mM TE/100 g FW, and voltammetry 141.0 mM TE/100 g FW for HHPE-UE method in free compound extraction. Regarding vitamin C content, its highest value was found by HHPE-UE (74 mg/100 g FW) a combined extraction method. The phenolic compounds rutin and p-coumaric acid were found in all the extracts, both in free and bound forms, respectively. Besides, the combined techniques improved the extraction of bioactive compounds.

Synthesis and Characterization of Chloridobis(dimethylglyoximato)4-(2-ferrocenylvinyl)pyridinecobalt(III): A New Heterobinuclear Cobaloxime

The synthesis of a new heterobinuclear cobaloxime with 4-(2-ferrocenylvinyl)pyridine, fcvpy, is reported. The complex [CoCl(dmgH)2(fcvpy)], where dmgH2 is dimethylglyoxime and dmgH is dimethylglyoximato, has been characterized by 1H-NMR, UV-Vis, cyclic voltammetry, and elemental analysis. The cyclic voltammogram of this complex shows a fc/fc

Apoptosis induced by a novel Ruthenium II complex in a Gallbladder carcinoma cell line

Some metals with striking properties have been used for the development of different complexes with activity against malignant cells. Due to difficulties in early diagnosis of gallbladder cancer (GBC) the main treatment option is chemotherapy. In this line of research, ruthenium complexes represent a less toxic alternative that others metal complexes such as cisplatin. The aim of this study was to evaluate the effect of a novel ruthenium complex in activation of apoptosis in a cell lineG-415. Our results show an activity of inducing cell death and over-expression of pro-apoptotic transcripts.

Selective cytotoxicity of a novel compound based on Ruthenium II in a Gallbladder carcinoma cell line

Gallbladder cancer (GBC) is highly prevalent in Chile and some southern regions of Colombia. This cancer presents low survival rate and poor prognosis in advanced stages. At this respect, the search for new drugs with better selective activity is necessary, among them ruthenium complexes represent an alternative for the development of more efficient drugs for the treatment of GBC. The objective of this study was to determine the in vitro cytotoxic activity of a novel complex based on Ruthenium (II) on the GBC cell line G-415 vs the non-tumor cell line HEK239 using MTT assay. The cell viability of the experiments showed a significant activity of the treatments in tumor cells.

Desactivación de la Fotoluminiscencia de un Complejo de Rutenio Anclado a Nanocristales de Dióxido de Titanio Empleando Cobaloximas

Resumen La desactivacion del estado excitado del complejo [Ru(deeb)(bpy) 2 ](PF 6 ) 2 (donde bpy es 2,2’-bipiridina y deeb es 4,4’-(COOEt) 2 2,2’-bipiridina) fue estudiada en disolucion de acetonitrilo y tambien anclado sobre una pelicula delgada de nanocristales de dioxido de titanio. Para desactivar el estado excitado del compuesto de rutenio se emplearon los complejos [Co(py)(Cl)(dmgH) 2 ] y [Co(py)(Bu)(dmgH) 2 ] (donde py es piridina, Bu es n-butilo y dmgH es dimetilglioxamato). Estas cobaloximas pueden desactivar de manera eficiente el estado excitado tanto en disolucion como soportado sobre dioxido de titanio. El proceso de desactivacion puede ser descrito mediante el modelo Stern-Volmer, cuyo analisis indica que el proceso de desactivacion esta dominado por procesos de difusion y es coherente con un proceso de transferencia de electrones foto inducido. Palabras clave: rutenio, cobaloximas, dioxido de titanio, estado excitado, modelo Stern-Volmer Photoluminiscence Quenching of Ruthenium Complexes Anchored to Nanocrystalline Titanium Dioxide using Cobaloximes