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Featured researches published by Amira Adel Taha Al-Hosary.


Journal of Parasitology Research | 2015

Assessment of the First Commercial ELISA Kit for the Diagnosis of Theileria annulata

Amira Adel Taha Al-Hosary; Jabbar Sabir Ahmed; Ann Nordengrahn; Malik Merza

The present study assesses the efficacy of SVANOVIR Theileria annulata-Ab, the first commercial ELISA kit for the diagnosis of Theileria annulata infection in cattle based on a recombinant protein known as T. annulata surface protein (TaSp). As a reference test, a polymerase chain reaction (PCR) assay depending on T. annulata merozoite surface antigen (Tams-1) was applied. A total of 468 blood samples as well as serum samples were randomly collected from cattle and tested in the PCR as well as in the ELISA developed in this study. Moreover, all samples were also analyzed by conventional Giemsa-stained blood smear. The results of this study revealed a good correlation between the results obtained by PCR and the ELISA, whereas all PCR positive samples scored correctly positive in the ELISA and 73 of the 125 PCR negative samples scored correctly negative. Taken together, a sensitivity of 91.25% and a specificity of 78.4% were recorded, when compared to the PCR data. In conclusion, the SVANOVIR Theileria annulata-Ab is a suitable diagnostic assay for use in the diagnosis and epidemiological surveys of Theileria annulata infection in chronic and carrier animals.


Ticks and Tick-borne Diseases | 2018

Epidemiological study on tropical theileriosis (Theileria annulata infection) in the Egyptian Oases with special reference to the molecular characterization of Theileria spp

Amira Adel Taha Al-Hosary; Laila S. Ahmed; Jabbar Sabir Ahmed; Ard M. Nijhof; Peter-Henning Clausen

Theileria annulata infection is a tick-borne disease known as Egyptian fever since 1947. It is a destructive obstacle for the livestock production in the Egyptian Oases (EL-Wady EL-Geded Province). The present study was conducted on 1068 cattle, ranged from below one year to more than eight years old; belonged to different farms and villages in EL-Wady EL-Geded Province. The infection was confirmed by blood smears, Tams-1 target based polymerase chain reaction (Tams-1 PCR), 18Ss rRNA polymerase chain reaction and semi nested-polymerase chain reaction (nPCR) followed by DNA sequencing and phylogenetic analyses, in addition to tick identification. Molecular techniques confirmed the infection in 63.6% (679/1068) of the examined animals while Giemsa-stained blood smears confirmed it in 36.8% (393/1062). Male and female animals showed molecular confirmed infection rates of 64.5 and 62.7%, respectively. Animals less than one year old were more infected (83.33%, 400/480) followed by animals less than three years (57.31%, 149/260) and animals less than five years (42.45%, 90/212), respectively. On the other hand, animal of five years old or above were less infected and the infection rate in this group was estimated to be 34.48% (40/116). Two tick species were identified during the present study: Hyalomma anatolicum and Rhipicephalus annulatus. Theileria annulata was the only Theileria species found in the Egyptian oases in respect to phylogenetic analysis of the obtained sequences.


Journal of Parasitic Diseases | 2014

Microscopical and serological studies on Sarcocystis infection with first report of S. cruzi in buffaloes (Bubalus bubalis) in Assiut, Egypt

Asmaa M. Metwally; Mahmoud R. Abd Ellah; Amira Adel Taha Al-Hosary; Mosaab A. Omar


Archive | 2010

Using Polymerase chain reaction (PCR) for Diagnosis of Bovine Theileriosis in Upper Egypt

Ahmed Abdel-Rady; Laila S. Ahmed; Amr Mohamed Mohamed; Amira Adel Taha Al-Hosary


Journal of Animal and Veterinary Advances | 2011

Comparison of Primer Sets for Amplification of 30 kDa Merozoite Surface Antigen of Bovine Theileriasis

Mahmoud R. Abd Ell; Amira Adel Taha Al-Hosary


Journal of Parasitic Diseases | 2017

Comparison between conventional and molecular methods for diagnosis of bovine babesiosis (Babesia bovis infection) in tick infested cattle in upper Egypt

Amira Adel Taha Al-Hosary


Journal of Advanced Veterinary and Animal Research | 2017

Detection and identification of Foot and Mouth disease virus serotypes in Assiut governorate, Egypt

Khaled Ahmed Sayed El-Khabaz; Amira Adel Taha Al-Hosary


International Journal for Agro Veterinary and Medical Sciences | 2010

Epidemiological studies on Bovine Theileriosis in Upper Egypt

Ahmed Abdel-Rady; Laila S. Ahmed; Amr Mohamed Mohamed; Amira Adel Taha Al-Hosary


Indian Journal of Animal Research | 2018

New Approach to Use Blood Smears for Diagnosis of Bovine Theileriosis.

M Merza; Amira Adel Taha Al-Hosary; A. Nordengrahn


Asian Journal of Animal and Veterinary Advances | 2018

Evaluation of Oxidative Stress in Sheep Infested with Ticks and Concurrent Diagnosis of Theileriosis

Mahmoud R. Abd Ellah; Amira Adel Taha Al-Hosary; Laila S. Ahmed

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