Amit Alexander Charan

Determination of heavy metals in water of Ganga and Yamuna river basin in Allahabad.

The objectives of this paper were to illustrate the distribution and levels of sediment contamination by heavy metals in the Allahabad city, and to compare recent data with those collected during the early 2001. The metals as, Cd, Ni, Cu, Fe, Pb, Co, Ni and Zn were chosen because of their abundance and toxic effects in the environment of highly industrialized and urbanized areas. The pollutants, which do not remain in water column or solution, could be absorbed rapidly by particulate matters and thereby they also could escape any detection by water monitoring schemes (Meiggs, 1980). Some heavy metals like Cd, Cu, Fe, Ni and Pb were determined in water from four reaches of the river from Yamuna (river-km 1112) upstream from Sangam to Arail Ghat (riverkm 851) downstream from Allahabad.

Antifungal Activity of Methanolic and Ethanolic Leaf Extracts of Medicinal Plants

The following plants were screened for thew study viz., Ocimum sanctum (Tulsi), Withania somnifera (Ashwagandha) and Asparagus racemosus (Shatavari) which were traditionally used in India to treat various diseases. In evaluating antioxidant property and phytochemical analysis, all three plants were screened for antifungal activity. It was evaluated using Well diffusion method. The extracts were tested against fungus Aspergillus niger. Inhibition of fungal growth was investigated using PDA well diffusion method. The contents of total flavonoid compounds in crude methanolic and ethanolic extracts obtained fromOcimum sanctum, Withania somnifera and Asparagus racemosus leaves. How to view point the article : Singh, Hariom Kumar, Prakash, Indra, Kumar, Varun, Gupta, Saurabh and Charan, Amit Alexander (2016). Antifungal activity of methanolic and ethanolic leaf extracts of medicinal plants. Internat. J. Plant Protec., 9(2) : 474-478,DOI : 10.15740/HAS/ IJPP/9.2/474-478.

To study the therapeutic role of Indian spices in the treatment of gastrointestinal disease caused by Vibrio species.

There are various human diseases caused by Vibrio species which are pathogenic in nature these diseases are mainly water-borne. In this case “To Study the Therapeutic Role of Indian Spices In The Treatment Of Gastrointestinal Disease Caused By Vibrio Species”. I have chosen fifteen types of Indian spices which are as follows Amomum subulatum, Brassica nigra, Capsicum annuum, Cinnamomum tamala, Cinnamomum verum, Coriandrum sativum, Cuminum cyminum,Elettaria cardamomum, Glycyrrhiza glabra, Trachyspermum ammi, Pimpinella anisum, Piper nigrum,Trigonella foenum-graecum, Curcuma longa, and Zingiber officinal and prepare their extract in three types of solvents (Ethanol, Methanol and Acetone) with the concentration of 70% against three Vibrio species (Vibrio cholera, Vibrio alginolyticus, Vibrio parahaemolyticus) by using agar well diffusion method.

Identification of vibriospecies from various water samples of Bareilly, using multiplex PCR

The purpose of this study was to investigate the prevalence of Human pathogenic vibrio species in water bodies of Bareilly, U.P. using the traditional culture and multiplex PCR methods. Vibrio species are very much prevalent in water bodies of tropical region. The study was conducted on 25 various water samples collected from rivers and ponds of Bareilly, U.P. For this, species specific primers were designed targeting the toxgene of the five pathogenic species. Confirmation was done using multiplex PCR technique for rapid detection of the five selected pathogenic species including V. cholera, V. alginolyticus, V. parahaemolyticus, V. mimicus and V. vulnificus . Out of the five targeted species three were present i.e. V. cholera, V. alginolyticus, V. parahaemolyticus . Water is essential to life, but many people do not have access to clean and safe drinking water and many die of waterborne bacterial infections. In this review a general characterization of the most important bacterial diseases transmitted through water like cholera is present.

Binding pattern determination for class of anti-alzheimer’s compound

Alzheimer’s disease (AD) is the most common form of dementia. No cure has been observed for the disease, and it worsens as it progresses, which eventually leads to death. It is believed that some plaques and tangles develop within the structure of the brain which causes brain cells to die. Alzheimer’s patients also have a deficiency of neurotransmitters which ultimately hampers the transmission of messages in the brain. It was confirmed by Amyloid hypothesisthat beta-amyloid (A) deposits are the fundamental cause of the disease. So, in this research natural and synthetic compounds were selected on the basis of their binding or inhibition to the amyloid precursor protein (APP). The protein and the ligands were optimized, docked and their interaction was visualized on the basis of binding energy.

Citric acid production from pre-treated sugarcane bagasse by Aspergillus niger under solid state fermentation

Citric acid is one of the important commercially produced organic acid. The aim of the present work was to study the utilization of pre-treated sugar cane bagasse for citric acid production using Aspergillus niger under solid state fermentation. The maximum value of citric acid was observed in acid treated substrate followed by urea and heat respectively. Fermentation conditions were also optimized and maximum production of citric acid occurred when the pH, Initial moisture content and temperature of the fermentation medium were 5, 65 per cent and 30°C, respectively. As a globally required organic acid for various industrial applications, citric acid can be produced at large scale by utilizing pre-treated agro residues such as sugarcane bagasse. Application of agro residues in the production of value added product can be a positive step towards agricultural waste management.