Amy Mok
University of Western Ontario
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Featured researches published by Amy Mok.
BJUI | 2012
Justin Zhu; Melanie Kalbfleisch; Yi Xin Yang; Relka Bihari; Ian Lobb; Michael Davison; Amy Mok; Gedaminas Cepinskas; Abdel-Rahman Lawendy; Alp Sener
Whats known on the subject? and What does the study add?
BJUI | 2012
Ian Lobb; Amy Mok; Zhu Lan; Weihua Liu; Bertha Garcia; Alp Sener
Whats known on the subject? and What does the study add?
FEBS Letters | 1992
Amy Mok; Gordon E. McDougall; W.C. McMurray
CDP‐diacylglycerol for polyglycerophosphatide biogenesis can be synthesized within rat liver mitochondria. This membrane‐associated enzyme was predominantly located in the inner mitochondrial membrane. GTP had a significant effect in activating the microsomal CDP‐diacylglycerol synthase, especially if the microsomes were preincubated with GTP in the presence of phosphatidic acid. This stimulatory effect of GTP on the microsomal enzyme was not detected in the mitochondrial fractions. The enzymes could be solubilized from the membrane fractions using CHAPS, and the detergent‐soluble activity partially restored by addition of phospholipids. Mitochondrial and microsomal CDP‐diacylglycerol synthase activity could be completely separated by anion‐exchange column chromatography. The mitochondrial and microsomal CDP‐diacylglycerol synthases appear to be two distinct enzymes with different localization and regulatory characteristics.
Biochimica et Biophysica Acta | 1989
Rudy A.W. Veldhuizen; Amy Mok; W.C. McMurray; Fred Possmayer
The potential involvement of the glycerophosphorylcholine (GPC) pathway for the synthesis of phosphatidylcholine (PC) has been examined in rat liver and lung and in a human line, the A549 cell which possesses characteristics representative of mature alveolar type II epithelial cells. Although mitochondrial and microsomal fractions from the above sources readily incorporated radioactive glycerophosphate into lipids, the only incorporation observed with radioactive GPC was a small variable labelling with the mitochondrial and microsomal fractions from rat lung. Even with these fractions, no radioactivity from GPC was incorporated into PC or lysoPC. Attempts to increase the incorporation of GPC into lipids by manipulating the incubation conditions were unsuccessful. It was concluded that the occurrence of the GPC pathway in liver and lung is unlikely.
The Journal of Urology | 2016
Jennifer Leigh; Manujendra Saha; Amy Mok; Omar Champsi; Rui Wang; Ian Lobb; Alp Sener
PURPOSE Anemia of end stage renal disease affects 90% of patients on hemodialysis and it is a tremendous concern of patients and health care providers. Renal disease creates a state of renal hypoxia, which may contribute to a lack of erythropoietin production from the kidney when low oxygen levels are sensed. This necessitates the use of exogenous erythropoietin preparations. MATERIALS AND METHODS Recent evidence suggests that endogenously derived hydrogen sulfide may mediate oxygen sensing in tissues. Given the known involvement of other small molecules such as nitric oxide in erythropoietin production and the observation of decreased urinary H2S levels in patients with renal failure, we postulated that H2S may be the primary mediator of erythropoietin production during hypoxia. PK1, 786-O and Hep3B cells were incubated in hypoxia (1% O2) for 24 hours. Hypoxic cells were treated with the H2S donor GYY 4137 and the H2S inhibitor hydroxylamine. Following hypoxia erythropoietin, HIF-1α, HIF-2α and CBS expression was measured by quantitative real-time polymerase chain reaction and Western blot. RESULTS Hydroxylamine administration led to a significant decrease in erythropoietin, HIF-1α, HIF-2α and CBS protein levels during hypoxia. This was rescued by administration of GYY 4137 for erythropoietin, CBS and HIF-2α. Additionally, CSE -/- mice placed in hypoxia for 72 hours showed decreased renal erythropoietin production compared to wild-type mice. CONCLUSIONS These data suggest previously undocumented interplay of the production and action of H2S during hypoxia with subsequent erythropoietin production. The use of novel hydrogen sulfide donors could represent an alternative to standard therapies of anemia of renal failure.
The Journal of Urology | 2016
Shouzhe Lin; Fazil Visram; Ian Lobb; Weihua Liu; Aaron Haig; Jifu Jiang; Amy Mok; Dameng Lian; Mark E. Wood; Matthew Whiteman; Alp Sener
FT-IR to determine the biochemical structure and the matrix material content. The matrix material component was calculated as percentage to total biochemical components. The stone microanalysis for heavy metals and trace elements contents (22 elements/stone) was carried out by using ICP-OES. The correlation between matrix material content, biochemical structure and heavy metals was evaluated. RESULTS: FT-IR analyses revealed; 268 (48.6%) Ca oxalate, 166 (30.15) uric acid, 68 (12.3%) mixed and 19 (3.4) Ca phosphate, 13 (2.4%) struvite and 12 (2.2%) cystine stones. ICP-OES showed significant increase in the concentrationof 15 elements (Al, B, Ba,Ca,Cd,Cr, K, Mg, Na, P, Pb, S, Se, Sr and Zn) within the examined urinary stones. Phosphate-based stones (Ca phosphate and Struvite) had the highest concentration of Al, B, Ba, Cr, K, Mg, Na, P, Pb, Sr and Zinc. Calciumbased (Oxalate) stones had high levels of Cd,Fe, Mo and Se. Uric acid stones had high concentration of S. The mean percentage of matrix material was 6.4%. 195 (35.3%) stones had zero matrix content. 190 (34.4%) had matrix material < 6.4%. Zero matrix stones had high concentration of heavymetals (Al, As,Ba,Ca,Cd,Cr, Cu, Fe,Mn,Mo,Naand P). High matrix-containing stones had only higher Se than low matrix. CONCLUSIONS: The human urinary stones contained a significant concentration of heavy and trace elements. Phosphate-based stones had highest concentration of HMTE. Uric acid stones had the least contents of HMTE. Matrix-rich stones had less contents of HMTE. Poor Matrix-containing stones had highest contents of HMTE. These results indicate that matrix material could play an equally important role in stone formation beside other micro-components specially HMTE.
Biochemistry and Cell Biology | 1993
Amy Mok; Gordon E. McDougall; W.C. McMurray
Biochemistry and Cell Biology | 1990
Amy Mok; W.C. McMurray
The Journal of Urology | 2016
Shouzhe Lin; Fazil Visram; Weihua Liu; Aaron Haig; Jifu Jiang; Amy Mok; Dameng Lian; Mark E. Wood; Robert Torregrossa; Matthew Whiteman; Ian Lobb; Alp Sener
Biochemistry and Cell Biology | 1988
Amy Mok; Tanya Wong; Octávio M. de Oliveira Filgueiras; Paul G. Casola; Don W. Nicholson; W.C. McMurray; Paul G.R. Harding; Fred Possmayer