Ana A. Feregrino-Pérez

Composition and Chemopreventive Effect of Polysaccharides from Common Beans (Phaseolus vulgaris L.) on Azoxymethane-Induced Colon Cancer

Common beans ( Phaseolus vulgaris L.) contain a high proportion of undigested carbohydrates (NDC) that can be fermented in the large intestine to produce short-chain fatty acids (SCFA) such as acetate, propionate, and butyrate. The objective of the present study was to evaluate the composition and chemopreventive effect of a polysaccharide extract (PE) from cooked common beans ( P. vulgaris L) cv. Negro 8025 on azoxymethane (AOM) induced colon cancer in rats. The PE induced SCFA production with the highest butyrate concentrated in the cecum zone: 6.7 +/- 0.06 mmol/g of sample for PE treatment and 5.29 +/- 0.24 mmol/g of sample for PE + AOM treatment. The number of aberrant crypt foci (ACF) and the transcriptional expression of bax and caspase-3 were increased, and rb expression was decreased. The data suggest that PE decreased ACF and had an influence on the expression of genes involved in colon cancer for the action of butyrate concentration.

Non-digestible fraction of cooked bean (Phaseolus vulgaris L.) cultivar Bayo Madero suppresses colonic aberrant crypt foci in azoxymethane-induced rats

The non-digestible fraction (NDF) of common bean (Phaseolus vulgaris L.) cultivar Bayo Madero was evaluated for its chemopreventive effect on azoxymethane (AOM) induced aberrant crypt foci (ACF) in rats. Diets containing cooked beans (CB) or its non-digestible fraction (NDF) were fed to 72 male rats after 2 azoxymethane injections (15 mg kg(-1) of body weight once a week for 2 weeks). ACF number, short chain fatty acids (SCFA) and β-glucuronidase activity were measured in colon sections from rats sacrificed 7 weeks after the last AOM injection. Food intake and weight gain of rats were unaffected by CB and NDF. CB and NDF suppressed the AOM-induced formation of ACF (0.8 and 1.5 ACF/distal zone, respectively vs. 6.6 ACF/distal zone based on methylene blue stain) and lowered β-glucuronidase activity in cecal, colonic and fecal content compared to AOM group. SCFA production was not significantly different among fecal, cecal and colonic content. These results indicate that CB and NDF from Bayo Madero provide direct chemoprotection against early stage of azoxymethane (AOM)-induced colon cancer in rats.

Current approaches for enhanced expression of secondary metabolites as bioactive compounds in plants for agronomic and human health purposes - a review.

The study of secondary metabolism in plants is an important source for the discovery of bioactive compounds with a wide range of applications. Today these bioactive compounds derived from plants are important drugs such as antibiotics, and agrochemicals substitutes, they also have been economically important as flavors and fragrances, dyes and pigments, and food preservatives. Many of the drugs sold today are synthetic modifications of naturally obtained substances. There is no rigid scheme for classifying secondary metabolites, but they can be divided into different groups based on their chemical components, function and biosynthesis: terpenoids and steroids, fatty acid-derived substances and polyketides, alkaloids, phenolic compounds, non-ribosomal polypeptides and enzyme cofactors. The increasing commercial importance of these chemical compounds has resulted in a great interest in secondary metabolism, particularly the possibility of altering the production of bioactive plant metabolites by means of tissue culture technology and metabolomics. In today’s world the use of bioactive compounds derived from plants plays an important role in pharmaceutical applications. This review presents information about these metabolites and their applications as well as their importance in agronomy and bioactive effects on human health as nutraceuticals. This review includes also the new tendencies to produce these bioactive compounds under different stresses conditions such as biotic and abiotic stress that could be included in production systems.

Elicitor Mixtures Significantly Increase Bioactive Compounds, Antioxidant Activity, and Quality Parameters in Sweet Bell Pepper

Sweet bell peppers are greatly appreciated for their taste, color, pungency, and aroma. Additionally, they are good sources of bioactive compounds with antioxidant activity, which can be improved by the use of elicitors. Elicitors act as metabolite-inducing factors (MIF) by mimic stress conditions. Since plants rarely experience a single stress condition one by one but are more likely to be exposed to simultaneous stresses, it is important to evaluate the effect of elicitors on plant secondary metabolism as mixtures. Jasmonic acid (JA), hydrogen peroxide (HP), and chitosan (CH) were applied to fruits and plants of bell pepper as mixtures. Bioactive compounds, antioxidant activity, and quality parameters were evaluated. The assessed elicitor cocktail leads to an increase in the variables evaluated (P ≤ 0.05) when applied to mature fruits after harvest, whereas the lowest values were observed in the treatment applied to immature fruits. Therefore, the application of the elicitor cocktail to harvested mature fruits is recommended in order to improve bioactive compounds and the antioxidant activity of sweet bell peppers.

Strategies for Sustainable Plant Food Production: Facing the Current Agricultural Challenges—Agriculture for Today and Tomorrow

Plants are fundamental elements of the human diet, either as direct sources of nutrients or indirectly as feed for animals. During the past years, the main goal of agriculture has been to increase yield in order to provide the food that is needed by a growing world population. However, as important as yield, but commonly forgotten in conventional agriculture, is to retain and, if possible, to increase the phytochemical content due to their health implications. By 2025, the global population will exceed 7 billion. In the short term, per capita availability of arable land and irrigation water will decrease from year to year while biotic and abiotic stresses expand. Food security, defined as economic, physical, and social access to a balanced diet and harmless drinking water will be a compromise, with a holistic approach to nutritional and non-nutritional factors needed to achieve success in the eradication of hunger. Science and technology will play a very important role in stimulating and sustaining agriculture leading to long-term increases in productivity without linked ecological harm.

Influence of hydrogen peroxide foliar applications on in vitro antimicrobial activity in Capsicum chinense Jacq.

Abstract The aim of this study was to evaluate the effect of foliar applications of hydrogen peroxide on the antimicrobial activity of Capsicum chinense Jacq. methanolic extracts. The effects of hydrogen peroxide application on metabolites accumulation of C. chinense var. Jaguar and var. Chichen Itza were evaluated. Total flavonoid and phenolic contents, as well as HPLC quantification of capsaicin and dihidrocapsaicin were carried out. Methanolic extracts were microbiologically tested against Staphylococcus aureus, Escherichia coli, Streptococcus mutants, Salmonella thompson, Listeria monocytogenes, Streptococcus faecalis, and Candida albicans. Total phenolics, flavonoids, and capsaicinoids contents in both varieties treated with hydrogen peroxide were found significantly higher as compared to control. The antibacterial activity of chili extracts was observed against Gram-positive and Gram-negative bacteria as well as anti-yeast. The results of in vitro antibacterial activity showed that hydrogen peroxide application increases the inhibitory effect against the pathogenic micro-organisms. Methanolic extracts of var. Jaguar, were the most active against S. aureus, S. Thompson, and C. albicans, while var. Chichen Itza was most potent against E. faecalis and E. coli. Thus, this study confirmed that metabolite-induced factors (MIFs) as hydrogen peroxide, increased secondary metabolites accumulation in C. chinense methanolic extracts and augmented their antimicrobial activity.

Effect of elicitors as stimulating substances on sensory quality traits in color sweet bell pepper (Capsicum annuum L. cv. fascinato and orangela) grown under greenhouse conditions

Abstract The effect of the application of salicylic acid (SA) and hydrogen peroxide (H2O2) was evaluated on sensory quality traits of two commercial sweet bell pepper varieties, Fascinato and Orangela, by descriptive sensory evaluation, principal component analysis (PCA), and Multivariate Analysis of Variance (MANOVA). A sensory descriptive lexicon was established for the sweet pepper and an intensity score was assigned for 25 sensory attributes in all the samples. Such intensity score was performed by twelve trained panelists. Among the results it was shown that foliar application of elicitors to pepper crop produced significant differences in sensory traits of the harvested fruits. MANOVA allowed detecting differences in aroma profile among treatments and varieties, showing important changes depending on the combination of elicitors applied on each variety. The principal component analysis (PCA), showed that the PC1 explained 81.02%, while PC2 explained 9.24% of the variance in the results. A strong correlation between varieties and treatments 2 (36 mM H2O2) and 4 (0.1 mM SA and 36 mM H2O2) with PC1 was observed. Treatments 2 and 4 were related to changes in aroma and flavor traits. Fascinato was more susceptible to the effect of elicitor than Orangela. Therefore, elicitor combinations used in this experiment were capable to improve sensory quality of peppers, and not only promote an increase in shelf life as previous studies have shown.

Role of Biotechnology in the Agrofood Industry

Abstract Biotechnology is the application of various scientific techniques to modify, improve, and increase the value of various foods, animal, plants, and microorganisms of economic interest. This discipline has been developed since ancient times, that is, the Aztecs or tribes of Chad used microorganisms, such as cyanobacteria for nutritional intake. During the first and second world war, yeasts were added to satisfy the nutritional needs of the population because of food shortages. In addition to this for several decades the molecular and genetic techniques, named today as “omics,” have brought great changes in foods, such as genetically modified foods. Likewise, the industry has been benefited because of the transformation of various food by using processing technology, the latter being one of the centers of high-tech 21st century. Within this processing technology, it is found that the automation systems have increased the quality of products, the production and the shelf life, reducing costs and material waste by human error, which impacts a better use of natural resources. However, the progress in the use of this science leads us to make decision schemes or real problems, such as what is the role of intellectual property to reward innovation and allow access to the technology developed or which are environmental risks to genetic modification? Thus, it is of great interest to evaluate the benefits and risks of biotechnology application in the agrofood industry.

Phytochemical and Pharmacological Properties of Secondary Metabolites in Berries

Abstract Berries belong to the best food sources of bioactive compounds, as they are phenolic compounds (phenolic acids, flavonoids, such as anthocyanins and flavonols, and tannins), and ascorbic acid, which forms the most representative composition of these bioactive compounds. Berries with the highest number of bioactive compounds are found in the varieties Rosaceae, Ericaceae, and so on. Therefore, the research suggests an inverse correlation between fruit intake and cancer development. The functional properties in berries are currently recognized and there is a wide consumption of these fruits. Currently, these compounds, either individually or in combination, are responsible for various health benefits of berries, such as prevention of inflammatory disorders, cardiovascular diseases, or protective effects to reduce the risk of various types of cancer, no precise results on the reduction of oxidative stress by modulating protein and lipid oxidation. Berries have a range of phytochemicals that are suggested to exert anticancer effects through different mechanisms. This chapter summarizes the biological compounds active in berries in relation to the prevention and treatment of cancer. Studies on bioavailability and potential toxicity of bioactive compounds also participate in this review. Finally, it is necessary to emphasize in relation in to plant crops and use of elicitors to increase their bioactive compounds and synthesis focused on nutrition and health.

Exogenous fragmented DNA acts as a damage-associated molecular pattern (DAMP) inducing changes in CpG DNA methylation and defence-related responses in Lactuca sativa

Damage-associated molecular patterns (DAMPs) have been studied recently to understand plant self-nonself recognition in response to attack by biotic and abiotic stresses. Extracellular DNA has emerged as a possible DAMP. As a DAMP DNA seems to function depending on the phylogenetic scale and has been tested in a few plant species. This study aimed to evaluate the possible role of self DNA (sDNA) as a DAMP by analysing changes in CpG DNA methylation and defence-related responses in lettuce (Lactuca sativa L.) as a model plant. sDNA and nonself DNA (nsDNA) from Capsicum chinense Murray (both species belong to the same clade, Asterids) stimulated aberrant seed germination and root growth in lettuce seedlings. Similar resultswere obtained with nsDNA obtained from Acaciella angustissima (Mill.) Britton & Rose plants (belonging to the clade Rosids I), although at significantly higher concentrations. Moreover, in most cases, this behaviour was correlated with hypomethylation of CpG DNA as well as defence responses measured as altered gene expression associated with oxidative burst and production of secondary metabolites (phenylpropanoids) related to coping with stress conditions. Our results suggested that extracellular and fragmented DNA has a role as a DAMP depending on phylogenetic closeness in plants as lettuce, inducing epigenetic, genetic and biochemical changes within the plant. The importance of our results is that, for the first time, they demonstrate that sDNA acts as a DAMP in plants, changing CpG DNA methylation levels as well as increasing the production of secondary metabolites associated with defence responses to stress.