Ana Capelo

Micropropagation of Juniperus phoenicea from adult plant explants and analysis of ploidy stability using flow cytometry

We report here the successful micropropagation of adult Juniperus phoenicea L. with respective ploidy stability studies. Microcuttings with axillary buds were grown on five media supplemented with different growth regulator combinations. Best elongation rates were achieved on Driver and Kuniyuki (DKW) medium supplemented with kinetin alone or with naphthaleneacetic acid (NAA), while Rugini olive (OM) medium stimulated the development of new branches. Shoots growing on Murashige and Skoog (MS) medium browned and showed necrotic zones. Shoots of second to fourth subcultures usually had higher elongation rates than those of the first culture. For rooting assays, half strength DKW and OM media, different concentrations of growth regulators, auxin continuous exposure vs. dipping and the type of solid matrix were assessed. During rooting assays, two morphotypes were observed with one type having well developed internodes and the other showing hyperhydratation and no internode development. High rooting rates (40 %) were only obtained in the first morphotype shoots exposed for 5 min to 2.4 µM IBA and then transferred to OM medium without growth regulators. Plants were acclimatized in pots containing a mixture of peat and Perlite (3:2) in greenhouse with progressive reduction of relative humidity. A flow cytometric screening for major ploidy changes revealed no differences among the morphotypes and between them and the mother plant. Also the nuclear DNA content of this species was estimated for the first time using flow cytometry (2C = 24.71 pg).

Education for sustainable development in East Timor

Purpose – The purpose of this paper is to explore the relevance of Education for Sustainable Development (ESD) in East Timor and proposes a set of ESD indicators to be implemented in the formal education sector. Design/methodology/approach – A detailed analysis of relevant literature concerning national development plans (Republica Democratica de Timor-Leste, 2011), literature centred in East Timor and international perspectives about ESD principles, policies and values (UN reports) was carried, in order to examine the relevance of ESD in East Timor and to assist the selection of a set of ESD indicators. These indicators are proposed in order to assess and monitor if the current national policies, educational programmes, secondary school content, learning goals and activities in formal education express concerns related with ESD promotion in terms of specific curricular themes, learning characteristics, learning resources, skills, values and attitudes. Findings – This paper argues that the East Timor gove...

Currículo do pré-secundário em Timor-Leste e sua articulação com o secundário geral

Problems inherited from the last decades have led Timor-Leste to restructure its pre-secondary – EPS – and general secondary – ESG – curricula. Considering the importance of an adequate vertical articulation throughout the schooling cycles and before the conclusion of the first complete edition of the EPS, it is necessary to study if such a curriculum is aligned with the one of the ESG, in particular with regard to Science and Technology. This article focuses on the analysis of the EPS curriculum and the evaluation of its articulation with the one of ESG. Notwithstanding the decalages among the prescribed, the implemented and the appropriate curricula, it is relevant to have a fine-tuning at this level to ensure good quality education and training, essential to achieve the millennium development goals.