Ana Filipa Ferreira

The ibex Capra pyrenaica returns to its former Portuguese range

The ibex Capra pyrenaica has recently recolonized its former Portuguese range from a contiguous Spanish protected area. The first observations of ibex in Portugal were in Peneda-Gerês National Park in 1998. In 2001 we began a survey to confirm ibex presence in Portuguese territory, and to determine the current status of the species there. There are three ibex nuclei in the general area of the international border, and they are expanding their geographical range with two of the nuclei almost restricted to Portuguese territory. In 2003 the ibex population consisted of a minimum of 75 individuals. Different founder histories have led to distinct age structures in each nucleus, but in general they exhibit the population dynamics typical of a recently reintroduced population with high reproductive potential. Priority conservation actions for this newly established population need to include increased monitoring, and reinforcement of the population with individuals from elsewhere. Effective conservation will require collaboration between staff of both Portuguese and Spanish protected areas and an integrated Spanish-Portuguese conservation plan.

Investigating population structure of Sea Lamprey (Petromyzon marinus, L.) in Western Iberian Peninsula using morphological characters and heart fatty acid signature analyses.

This study hypothesizes the existence of three groups of sea lamprey Petromyzon marinus L. in Portugal (North/Central group, Tagus group, and Guadiana group), possibly promoted by seabed topography isolation during the oceanic phase of the life cycle. Within this context, our purpose was to analyze the existence of a stock structure on sea lamprey populations sampled in the major Portuguese river basins using both morphological characters and heart tissue fatty acid signature. In both cases, the multiple discriminant analysis revealed statistically significant differences among groups, and the overall corrected classification rate estimated from cross-validation procedure was particularly high for the cardiac muscle fatty acid profiles (i.e. 83.8%). Morphometric characters were much more useful than meristic ones to discriminate stocks, and the most important variables for group differentiation were eye length, second dorsal fin length and branchial length. Fatty acid analysis showed that all lampreys from the southern Guadiana group were correctly classified and not mixing with individuals from any other group, reflecting a typical heart fatty acid signature. Our results revealed that 89.5% and 72.2% of the individuals from the Tagus and North/Central groups, respectively, were also correctly classified, despite some degree of overlap between individuals from these groups. The fatty acids that contributed to the observed segregation were C16:0; C17:0; C18:1ω9; C20:3ω6 and C22:2ω6. Detected differences are probably related with environmental variables to which lampreys may have been exposed, which leaded to different patterns of gene expression. These results suggest the existence of three different sea lamprey stocks in Portugal, with implication in terms of management and conservation.

Structural lipid changes and Na(+)/K(+)-ATPase activity of gill cells' basolateral membranes during saltwater acclimation in sea lamprey (Petromyzon marinus, L.) juveniles.

Seawater acclimation is a critical period for anadromous species and a process yet to be understood in lampreys. Considering that changes in lipid composition of the gill cells basolateral membranes may disrupt the major transporter Na(+)K(+)-ATPase, the goal of this study was to detect changes at this level during juvenile sea lamprey seawater acclimation. The results showed that saltwater acclimation has a direct effect on the fatty acid composition of gill cells basolateral membranes phospholipids. When held in full-strength seawater, the fatty acid profile of basolateral membranes phospholipids suffered a restructure by increasing either saturation or the ratio between oleic acid and eicosapentaenoic acid. Simultaneously, the activity of Na(+)K(+)-ATPase revealed a significant and positive correlation with basolateral membranes cholesterol content in the presence of highest salinity. Our results pointed out for lipid adjustments involving the functional transporter present on the gill cell basolateral membranes to ensure the role played by branchial Na(+)K(+)-ATPase in ion transport during saltwater acclimation process. The responses observed contributed to the strategy adopted by gill cells basolateral membranes to compensate for osmotic and ionic stressors, to ensure the success of the process of seawater acclimation associated with the downstream trophic migration of juvenile sea lamprey.

Does livestock influence the diet of Iberian ibex Capra pyrenaica in the Peneda-Gerês National Park (Portugal)?

Abstract We carried out a pilot study about the influence of the domestic goat Capra hircus on the feeding ecology of the Iberian ibex Capra pyrenaica in the Peneda-Gerês National Park (PGNP, Portugal). We mapped the grazing areas of domestic goat flocks and determined their stocking rates in two mountains of PGNP, Gerês and Amarela, and we investigated the diets of both ungulates using fecal microhistology in an area where they partially overlapped during spring and summer. Diet composition, ligneous diversity, and ligneous trophic niche were compared seasonally. Although differences in ligneous components were observed, results revealed a considerable diet overlap mainly due to graminoids consumption, especially during autumn and spring. This suggests that direct or indirect foraging competition between the goat species should not be discarded if they spatially concur. Results also emphasized distinct summer feeding strategies of C. pyrenaica in the area, and the influence of cattle (Bos taurus) and horses (Equus caballus) were hypothesized. We recommend removing feral goats from the mountain environment and monitoring grazing areas of domestic flocks. Estimates of ecological carrying capacity and future diet studies should include cattle, horses, and improvement of herb identification.

Can mitochondrial malondialdehyde content be a useful tool to evaluate sea lamprey juveniles’ capacity to seawater acclimatization?

Abstract The sea lamprey is an anadromous species that migrates twice during its life cycle between freshwater and seawater. Microphagous larvae generally spend 4–5 years burrowed in the substrate of rivers and streams before undergoing metamorphosis that ends with the beginning of the juvenile trophic migration. Once metamorphosis is complete, sea lamprey juvenile downstream migrants are fully tolerant to seawater salinity. Pollution resulting from industrial effluents may disturb the seawater acclimatization causing oxidative damage, and ultimately may lead to a decrease of sea lamprey population. The aim of this study was to compare salt acclimation of sea lamprey juveniles captured in river basins with different levels of aquatic pollution, using mitochondrial glutathione (GSH) and malondialdehyde (MDA) of gills and liver as markers of physiological stress and cell damage. The results showed that juveniles from the Lima basin exhibited the highest levels of mitochondrial MDA in gills, even though significant changes in the stress markers of mitochondrial gills of all animals subject to salt acclimation were not detected. In addition, an increase in the oxidative damage of hepatic mitochondria of macrophthalmia from the Vouga basin suggests the occurrence of metabolic failures with the potential to disturb the capacity to adaptation to the marine environment.

Seasonal dietary shifts and selection of Iberian wild goat Capra pyrenaica Schinz, 1838 in Peneda-Gerês National Park (Portugal)

This study intended to know Iberian wild goat Capra pyrenaica Schinz, 1838 feeding strategy in two proximate mountains it recently recolonized, Geres and Amarela (Peneda-Geres National Park, PGNP, Portugal). For that purpose we studied species dietary composition using faecal diet microhistological determinations and also its diet selection. Albeit wild goat exhibited an intermediate browse - graze behaviour in the two areas, grazing was more pronounced in Geres while browsing in Amarela. Both areas presented a dietary shift in spring consisting in an increase on the consumption and preference for graminoids. This feeding strategy extended through summer only in Amarela. Results obtained are congruent with wild goat generalist feeding behaviour in other regions of the Iberian Peninsula and suggest that species feeding strategy in PGNP respond to spatial patterns of resources, specifically of graminoids, and to livestock stocking rates and management.