Network


Latest external collaboration on country level. Dive into details by clicking on the dots.

Hotspot


Dive into the research topics where Ana Paula Pinto is active.

Publication


Featured researches published by Ana Paula Pinto.


Lasers in Medical Science | 2018

Biofilm formation by Candida albicans is inhibited by photodynamic antimicrobial chemotherapy (PACT), using chlorin e6: increase in both ROS production and membrane permeability

Moisés Lopes Carvalho; Ana Paula Pinto; Leandro Raniero; Maricilia Silva Costa

Candida albicans is an opportunistic fungal producing both superficial and systemic infections in immunocompromised patients. Furthermore, it has been described an increase in the frequency of infections which have become refractory to standard antifungal therapy. Photodynamic antimicrobial chemotherapy (PACT) is a potential antimicrobial therapy that combines visible light and a nontoxic dye, known as a photosensitizer, producing reactive oxygen species (ROS) that can kill the treated cells. The objective of this study was to investigate the effects of PACT, using chlorin e6, as a photosensitizer on C. albicans. In this work, we studied the effect of PACT on both cell growth and biofilm formation by C. albicans. In addition, both ROS production and cell permeability were determined after PACT. PACT inhibited both growth and biofilm formation by C. albicans. We have also observed that PACT increased both ROS production (six times) and cell membrane permeability (five times) in C. albicans. PACT decreased both cell growth and biofilm development. The effect of PACT using chlorin e6 on C. albicans could be associated with an increase in ROS production, which could increase cell permeability, producing permanent damage to the cell membranes, leading to the cell death.


Photodiagnosis and Photodynamic Therapy | 2018

Photodynamic Antimicrobial Chemotherapy (PACT), using Toluidine blue O inhibits the viability of biofilm produced by Candida albicans at different stages of development

Ana Paula Pinto; Isabela Bueno Rosseti; Moisés Lopes Carvalho; Bruna Graziele Marques da Silva; Carlos Alberto-Silva; Maricilia Silva Costa

BACKGROUND Candida albicans is an opportunistic fungus producing both superficial and systemic infections, especially in immunocompromised individuals. It has been demonstrated that C. albicans ability to form biofilms is a crucial process for colonization and virulence. Furthermore, a correlation between the development of drug resistance and biofilm maturation at Candida biofilms has been shown. Photodynamic Antimicrobial Chemotherapy (PACT) is a potential antimicrobial therapy that combines visible light and a non-toxic dye, known as a photosensitizer, producing reactive oxygen species (ROS) that can kill the treated cells. The objective of this study was to investigate the effects of PACT, using Toluidine Blue O (TBO) on the viability of biofilms produced by C. albicans at different stages of development. METHODS In this study, the effects of PACT on both biofilm formation and viability of the biofilm produced by C. albicans were studied. Biofilm formation and viability were determined by a metabolic assay based on the reduction of XTT assay. In addition, the morphology of the biofilm was observed using light microscopy. RESULTS PACT inhibited both biofilm formation and viability of the biofilm produced by C. albicans. Furthermore, PACT was able to decrease the number of both cells and filamentous form present in the biofilm structure. This inhibitory effect was observed in both early and mature biofilms. CONCLUSIONS The results obtained in this study demonstrated the potential of PACT (using TBO) as an effective antifungal therapy, including against infections associated with biofilms at different stages of development.


Revista UniVap | 2017

ESTUDO DO CRESCIMENTO DE Candida spp. COM O MODELO DE VERHULST E REGRESSÃO LINEAR

Laís Mayara Machado de Amorim; Moisés Lopes Carvalho; Ana Paula Pinto; Francielzo Ferreira Lima; Moacira Lopes Carvalho; Rodrigo S. Pessoa


Revista UniVap | 2017

EFEITOS DA LASERTERAPIA DE BAIXA INTENSIDADE E EXTRATOS VEGETAIS NO PROCESSO DE REPARO TECIDUAL: REVISÃO INTEGRATIVA

Ana Paula Pinto; Moisés Lopes Carvalho; Laís Mayara Machado de Amorim; Francielzo Ferreira Lima; Moacira Lopes Carvalho; Renata Amadei Nicolau


Revista UniVap | 2017

SUICÍDIO EM IDOSOS NO BRASIL: uma revisão integrativa

Ana Paula Pinto; Moisés Lopes Carvalho; Laís Mayara Machado de Amorim; Regina Carvalho Linhares; Francielzo Ferreira Lima; Moacira Lopes Carvalho; Krieger Rhelyni de Sousa Olinda


Revista UniVap | 2017

EFEITO ANTIFÚNGICO DO RESVERATROL SOBRE A CANDIDA ALBICANS

Bruna Graziele Marques da Silva; Moisés Lopes Carvalho; Ana Paula Pinto; Maricilia Silva Costa


Revista UniVap | 2017

ESTUDO DO EFEITO DA TERAPIA FOTODINÂMICA ANTIMICROBIANA SOBRE A VIABILIDADE DO BIOFILME PRODUZIDO POR Candida albicans

Ana Paula Pinto; Isabela Bueno Rosseti; Maricilia Silva Costa


Revista Interdisciplinar | 2017

Identificação rápida de candida albicans por espectroscopia vibracional: uma análise de evidências internacionais

Juliana dos Santos Corrêa; Moisés Lopes Carvalho; Larissa Vanessa Machado Viana; Francielzo Ferreira Lima; Laís Mayara Machado de Amorim; Ana Paula Pinto


Revista Interdisciplinar | 2017

Sistematizando o cuidado ao paciente com prostatite: relato de caso clínico

Moisés Lopes Carvalho; Maria do Rosário de Fátima Franco Batista; Ellen Thallita Hill Araújo; Ana Paula Pinto; Moacira Lopes Carvalho; Luana Rodrigues da Silva


Revista Interdisciplinar | 2016

Análise do crescimento de Candida albicans e Candida krusei com diferentes modelos matemáticos

Moisés Lopes Carvalho; Laís Mayara Machado de Amorim; Ana Paula Pinto; Francielzo Ferreira Lima; Moacira Lopes Carvalho; Rodrigo S. Pessoa

Collaboration


Dive into the Ana Paula Pinto's collaboration.

Top Co-Authors

Avatar

Laís Mayara Machado de Amorim

Universidade Federal do Espírito Santo

View shared research outputs
Top Co-Authors

Avatar

Rodrigo S. Pessoa

Instituto Tecnológico de Aeronáutica

View shared research outputs
Top Co-Authors

Avatar
Top Co-Authors

Avatar

Maricilia Silva Costa

Federal University of São Paulo

View shared research outputs
Researchain Logo
Decentralizing Knowledge