Ana Paula Pinto
University of Paraíba Valley
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Publication
Featured researches published by Ana Paula Pinto.
Lasers in Medical Science | 2018
Moisés Lopes Carvalho; Ana Paula Pinto; Leandro Raniero; Maricilia Silva Costa
Candida albicans is an opportunistic fungal producing both superficial and systemic infections in immunocompromised patients. Furthermore, it has been described an increase in the frequency of infections which have become refractory to standard antifungal therapy. Photodynamic antimicrobial chemotherapy (PACT) is a potential antimicrobial therapy that combines visible light and a nontoxic dye, known as a photosensitizer, producing reactive oxygen species (ROS) that can kill the treated cells. The objective of this study was to investigate the effects of PACT, using chlorin e6, as a photosensitizer on C. albicans. In this work, we studied the effect of PACT on both cell growth and biofilm formation by C. albicans. In addition, both ROS production and cell permeability were determined after PACT. PACT inhibited both growth and biofilm formation by C. albicans. We have also observed that PACT increased both ROS production (six times) and cell membrane permeability (five times) in C. albicans. PACT decreased both cell growth and biofilm development. The effect of PACT using chlorin e6 on C. albicans could be associated with an increase in ROS production, which could increase cell permeability, producing permanent damage to the cell membranes, leading to the cell death.
Photodiagnosis and Photodynamic Therapy | 2018
Ana Paula Pinto; Isabela Bueno Rosseti; Moisés Lopes Carvalho; Bruna Graziele Marques da Silva; Carlos Alberto-Silva; Maricilia Silva Costa
BACKGROUND Candida albicans is an opportunistic fungus producing both superficial and systemic infections, especially in immunocompromised individuals. It has been demonstrated that C. albicans ability to form biofilms is a crucial process for colonization and virulence. Furthermore, a correlation between the development of drug resistance and biofilm maturation at Candida biofilms has been shown. Photodynamic Antimicrobial Chemotherapy (PACT) is a potential antimicrobial therapy that combines visible light and a non-toxic dye, known as a photosensitizer, producing reactive oxygen species (ROS) that can kill the treated cells. The objective of this study was to investigate the effects of PACT, using Toluidine Blue O (TBO) on the viability of biofilms produced by C. albicans at different stages of development. METHODS In this study, the effects of PACT on both biofilm formation and viability of the biofilm produced by C. albicans were studied. Biofilm formation and viability were determined by a metabolic assay based on the reduction of XTT assay. In addition, the morphology of the biofilm was observed using light microscopy. RESULTS PACT inhibited both biofilm formation and viability of the biofilm produced by C. albicans. Furthermore, PACT was able to decrease the number of both cells and filamentous form present in the biofilm structure. This inhibitory effect was observed in both early and mature biofilms. CONCLUSIONS The results obtained in this study demonstrated the potential of PACT (using TBO) as an effective antifungal therapy, including against infections associated with biofilms at different stages of development.
Revista UniVap | 2017
Laís Mayara Machado de Amorim; Moisés Lopes Carvalho; Ana Paula Pinto; Francielzo Ferreira Lima; Moacira Lopes Carvalho; Rodrigo S. Pessoa
Revista UniVap | 2017
Ana Paula Pinto; Moisés Lopes Carvalho; Laís Mayara Machado de Amorim; Francielzo Ferreira Lima; Moacira Lopes Carvalho; Renata Amadei Nicolau
Revista UniVap | 2017
Ana Paula Pinto; Moisés Lopes Carvalho; Laís Mayara Machado de Amorim; Regina Carvalho Linhares; Francielzo Ferreira Lima; Moacira Lopes Carvalho; Krieger Rhelyni de Sousa Olinda
Revista UniVap | 2017
Bruna Graziele Marques da Silva; Moisés Lopes Carvalho; Ana Paula Pinto; Maricilia Silva Costa
Revista UniVap | 2017
Ana Paula Pinto; Isabela Bueno Rosseti; Maricilia Silva Costa
Revista Interdisciplinar | 2017
Juliana dos Santos Corrêa; Moisés Lopes Carvalho; Larissa Vanessa Machado Viana; Francielzo Ferreira Lima; Laís Mayara Machado de Amorim; Ana Paula Pinto
Revista Interdisciplinar | 2017
Moisés Lopes Carvalho; Maria do Rosário de Fátima Franco Batista; Ellen Thallita Hill Araújo; Ana Paula Pinto; Moacira Lopes Carvalho; Luana Rodrigues da Silva
Revista Interdisciplinar | 2016
Moisés Lopes Carvalho; Laís Mayara Machado de Amorim; Ana Paula Pinto; Francielzo Ferreira Lima; Moacira Lopes Carvalho; Rodrigo S. Pessoa