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Dive into the research topics where Ana Paula Testa Pezzin is active.

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Featured researches published by Ana Paula Testa Pezzin.


Materials Research-ibero-american Journal of Materials | 2003

Blendas biodegradáveis de poli(3-hidroxibutirato)/poli(e-caprolactona): obtenção e estudo da miscibilidade

Nilton Vogelsanger; Michele Formolo; Ana Paula Testa Pezzin; Andréa Lima dos Santos Schneider; Sandra Aparecida Furlan; Heloísa Pinna Bernardo; Sérgio Henrique Pezzin; A.T.N. Pires; Eliana Aparecida de Rezende Duek

Due to its biodegradability, poly(3-hydroxybutyrate) P(3-HB) has attracted much attention in the environmental sector. However, some characteristics of this polymer, such as high crystallinity, poor processability and high brittleness, have lead several research groups to study polymeric blends in order to modify P(3-HB) properties. Poly(e-caprolactone) (PCL) is a synthetic polyester which is completely degraded after about one year when buried in soil. In general, it acts as a polymeric plasticizer lowering the elastic modulus and enhancing the processability of the blend. Blends of two biodegradable polymers, P(3-HB) and PCL have been prepared by casting in different compositions. Miscibility, thermal behavior and morphology of these blends were studied using modulated differential scanning calorimetry (MDSC), scanning electron microscopy (SEM) and polarizing light microscopy (PLM). The two glass transition temperatures, detected by MDSC, suggest the immiscibility of the system. Phase separation was confirmed by PLM.


Polimeros-ciencia E Tecnologia | 2002

Estudo da Degradação In Vitro de Blendas de Poli(p-dioxanona)/Poli(l-Ácido Láctico) (PPD/PLLA) Preparadas por Evaporação de Solvente

Ana Paula Testa Pezzin; Cecília A.C. Zavaglia; Eliana Ap. R. Duek

Blends of two crystalline bioreabsorbable polymers, poly(L-lactic acid) (PLLA) and poly(p- dioxanone) (PPD), have been prepared by solvent casting in different compositions. The blends were immersed in tubes containing a phosphate buffer solution (pH = 7.4) in a thermally controlled bath of 37 ± 1°C and studied by differential scanning calorimetry (DSC), thermogravimetric analysis (TGA) and scanning electron microscopy (SEM). It was observed by in vitro study that PLLA has a slower degradation rate than PPD and the blends presented an intermediate degradation rate. This shows that it is possible to vary the degradation rate of the blend by changing its composition.


BMC Proceedings | 2014

Vacuum dried membranes of poly (l-lactic acid) and bacterial cellulose for biomedical applications

Drielle Justiniano de Souza; Luismar Marques Porto; Ana Paula Testa Pezzin

Background Periodontitis is the inflammation of tissues supporting the tooth when gingivitis is not treated [1]. The treatment should reduce/eliminate inflammation of the tissue, which is induced by the biofilm with its byproducts and correct anatomic defects caused by the disease [2]. Guided tissue regeneration is a technique that applies membranes that form a blood clot that may allow the growth and differentiation of cells and regenerate the periodontal tissues. This should be able to block and isolate the area and contribute passively to tissue integration with guaranteed protection, including possible bacterial contamination [2,3]. This work aimed to obtain biocomposites of poly(l-lactic acid) (PLLA) with bacterial cellulose (BC) by different methodologies to be applied as membranes for the treatment of periodontitis. The characterization was performed by scanning electron microscopy, cell adhesion and contact angle. It was observed that the membranes 80/20 prepared by casting prior to vacuum drying of BC exhibited better adhesion. This membrane proved to be non-cytotoxic and more hydrophilic.


Polimeros-ciencia E Tecnologia | 2010

Citocompatibilidade de blendas de poli(p-dioxanona)/ poli(hidroxi butirato) (PPD/PHB) para aplicações em engenharia de tecido cartilaginoso

Andrea Rodrigues Esposito; Eliana A. R. Duek; Carolina Lucchesi; Luciana Prazeres; Ana Paula Testa Pezzin

Buscando estrategias que repercutam na melhoria da interacao entre materiais polimericos biorreabsorviveis e o crescimento celular, o presente estudo in vitro teve como objetivo estudar a influencia de blendas de PPD/PHB na adesao celular e crescimento de fibrocondrocitos obtidos a partir de cultura primaria. As blendas de PPD/PHB foram preparadas pelo metodo de evaporacao de solvente nas composicoes 100/0, 60/40 e 50/50 e caracterizadas por microscopia eletronica de varredura (MEV). Observacoes ultra-estruturais mostraram alteracoes na morfologia celular, sugerindo que os fibrocondrocitos podem responder a alteracoes no substrato alterando seu perfil fenotipico. As analises com MTT demonstraram que as blendas nao apresentaram citotoxicidade e permitiram a adesao e proliferacao dos fibrocondrocitos sobre os substratos em todas as suas composicoes. O ensaio colorimetrico com Sirius Red evidenciou a capacidade de manutencao da sintese de matriz extracelular colagena sobre as amostras, concluindo-se que as blendas de PPD/PHB podem ser indicadas para o cultivo celular.


Polimeros-ciencia E Tecnologia | 2017

Produção de poli(3-hidroxibutirato) por Cupriavidus necator em batelada alimentada usando glicerol

Bruna Regina Sombrio; Andréa Lima dos Santos Schneider; Ana Paula Testa Pezzin; Giannini Paszinick Apati; Tamiris Schroeder

Poly(3-hydroxybutyrate) [P(3HB)] is a natural, biodegradable polyester and is considered an attractive substitute for the petrochemical polymers, because has advantage of being degraded in the soil within a few months by microorganisms. This work exploits three strategies to synthesize P(3HB) from of the Cupriavidus necator with glycerol as co-substrate: cultivation without glycerol, with addition of 20 g L–1 glycerol in the phase of polymer producing and addition of 20 g L–1 of glycerol at the start of cultivation, in order to evaluate its effect on cell growth and the synthesis of the polymer. The results showed that the addition of glycerol at the start of culture led to highest values of accumulation percentage the P(3HB) (64.12%). However, in the experiments without glycerol can be observed higher values toward biomass to substrate yield (0.17 g g–1). These parameters presented statistically significant differences in function of the cultivation strategy used.


Polimeros-ciencia E Tecnologia | 2016

Microencapsulação do pesticida cipermetrina em blendas de P(3HB/PCL): caracterização e ensaio de liberação in vitro

Evana Cássia Dall’Agnol; Jaqueline Suave; Marcia Margarete Meier; Valdir Soldi; Denise Abatti Kasper Silva; Ana Paula Testa Pezzin

A aplicacao de polimeros biodegradaveis para encapsular pesticidas e uma estrategia que permite, a partir de diferentes proporcoes entre os polimeros, modificar o perfil de liberacao do agente. Este trabalho avaliou a liberacao controlada do pesticida cipermetrina encapsulado em microesferas de blendas de P(3HB)/PCL (100/0, 0/100, 97/03, 95/05, 90/10, 80/20 e 70/30) obtidas pelo metodo de emulsificacao-evaporacao do solvente. As imagens de microscopia eletronica de varredura revelam a forte influencia da PCL na porosidade das microesferas. As analises de infravermelho mostraram a presenca do pesticida em todas as composicoes de polimeros avaliadas. O ensaio de liberacao de cipermetrina sugere que, no intervalo de 4 horas, o teor de cipermetrina liberada e dependente da composicao das blendas utilizadas. As microesferas de PHB e blendas com menores teores de PCL, 97/03 e 95/05 liberaram 75% a 85% enquanto a composicao 70/30 liberou 100% de cipermetrina.


Artificial Organs | 2003

Bioreabsorbable Polymer Scaffold as Temporary Meniscal Prosthesis

Ana Paula Testa Pezzin; Tulio Pereira Cardoso; Maria do Carmo Alberto Rincón; Cecília A.C. Zavaglia; Eliana Aparecida de Rezende Duek


Materials Research-ibero-american Journal of Materials | 2006

Thermal and mechanical behavior of injection molded Poly(3-hydroxybutyrate)/Poly(epsilon-caprolactone) blends

Marcia Adriana Tomaz Duarte; Roberson Goulart Hugen; Eduardo Sant'Anna Martins; Ana Paula Testa Pezzin; Sérgio Henrique Pezzin


Polimeros-ciencia E Tecnologia | 2013

Influence of poly(ethylene grycol) - (PEG) on the properties of influence of poly(3-hydroxybutyrate-CO-3-hydroxyvalerate) - PHBV

Sara E. M. Catoni; Ketlyn N. Trindade; Caio A. T. Gomes; Andréa Lima dos Santos Schneider; Ana Paula Testa Pezzin; Valdir Soldi


Materials Science and Engineering: C | 2009

Strategies of biosynthesis of poly(3-hydroxybutyrate) supplemented with biodiesel obtained from rice bran oil

L.P. Mazur; D.D. da Silva; Vitor Henrique Grigull; Michele Cristina Formolo Garcia; T.O. Magalhães; T.M. Wagner; S. Einloft; J. Dullius; Andréa Lima dos Santos Schneider; Ana Paula Testa Pezzin

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Eliana Aparecida de Rezende Duek

Pontifícia Universidade Católica de São Paulo

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Sérgio Henrique Pezzin

Universidade do Estado de Santa Catarina

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Carolina Lucchesi

State University of Campinas

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Eduardo Sant'Anna Martins

Universidade do Estado de Santa Catarina

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Eliana A. R. Duek

State University of Campinas

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Eliana Ap. R. Duek

State University of Campinas

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J. Dullius

Pontifícia Universidade Católica do Rio Grande do Sul

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