Ana Vasic

Classical-neural and quantum-holographic informatics: Psychosomatic-cognitive implications

Modeling of psychosomatic-cognitive functions is considered, in the framework of combined hierarchical brainpsilas and bodypsilas acupuncture neural networks. It is pointed out that presented models of brainpsilas hierarchical neural networks demonstrate encourageing advances in modeling cognitive functions. However, for modeling most cognitive and psychosomatic functions, the subtle biophysical quantum-holographic microwave Hopfield bodypsilas acupuncture neural networks are also necessary. On the one hand, they demonstrate existance of two cognitive modes of consciousness, and on the other hand, they represent natural framework for explaining contemporary meridian (psycho)therapies for very fast removing of psychosomatic disorders, demonstrating close relationship between acupuncture system and consciousness - with significant psychosomatic and transpersonal implications.

Seroprevalence of Ehrlichia Canis Infection in Stray Dogs from Serbia

Abstract Canine Monocytic Ehrlichiosis is a zoonotic bacterial disease with worldwide distribution. With regards to the population of stray dogs, the disease is facilitated due to their lifestyle and the lack of anti-parasitic protection. The aim of this study was to provide serological data on the presence of a specific Ehrlichia canis IgG antibodies in stray dogs, originating from 7 municipalities in Serbia. During the period from April 2013 to June 2014, 217 canine sera were submitted to the laboratory of the Department of Infectious Diseases of Animals and Bees, Faculty of Veterinary Medicine in Belgrade. An immunofluorescent antibody test (IFAT) was performed to detect antibodies to Ehrlichia canis (cut off, 1:50). Seropositive dogs were found in 5 out of 7 counties with a seroprevalence varying from 3.57% to 20% and an overall seroprevalence of 11.06% (24/217). There was no statistically significant difference between the prevalence of infection and the host age or gender. Results showed that stray dogs contribute to maintaining and spreading of Ehrlichia canis in Serbia. Due to the close relationship between people and dogs, it is of great importance to constantly monitor and improve prevention of this disease.

TICK POPULATION IN GOATS AND SHEEP IN ŠABAC

Abstract During our examination performed in the period from 2010 to 2012, we collected ticks from 52 flocks of sheep and 38 goat flocks. Ticks infestation occured in 15.97% (214/1340) of sheep and 16.93% (107/632) of goats. The result showed the presence of Ixodes ricinus, Rhipicephalus sanguineus, R. bursa, Dermacentor marginatus, D. pictus, Haemaphysalis punctata and Ha. inermis. Additional to determination of tick species during the research, the sex ratio and the monthly influence of microclimate conditions (temperature, relative humidity and precipitation quantity) on the dynamics of populations of ticks were followed. Obtained results indicate the importance of the impact of climatic factors on the population dynamics of some species of ticks as well as the dynamics and abundance of different sexes within established species of ticks.

Tick infestation and occurrence of Anaplasma phagocytophilum and piroplasms in cattle in the Republic of Serbia

Ticks transmit important pathogens affecting cattle such as intracellular bacteria of the genus Anaplasma or protozoa of the order Piroplasmida. This study aimed at assessing tick species present on pastures and cattle and determining occurrence of the tick-borne pathogens Anaplasma phagocytophilum and Babesia spp. in cattle. During May and June 2013, ticks and EDTA blood were collected from 185 dairy cows at six locations in Serbia. Ticks were also collected directly from the pastures at four of these locations. The occurrence of A. phagocytophilum was investigated by serological (immunofluorescence antibody test (IFAT)) and molecular methods (real-time PCR) and of piroplasms by traditional PCR followed by sequencing. The most prevalent tick species on animals was Ixodes ricinus, (n = 167), followed by Haemaphysalis punctata (n = 146) and Dermacentor marginatus (n = 122). On the pasture, however, the most common species was H. punctata (n = 41), I. ricinus (n = 37), and D. marginatus (n = 2). Altogether, 4 out of 163 (2.45%) serum samples of cows were positive for A. phagocytophilum-specific antibodies by IFAT. However, the 135 blood samples tested for A. phagocytophilum DNA were all negative. Altogether, 5 out of these 135 samples were positive for piroplasm DNA. PCR products were sequenced and identified as a benign Theileria spp. with 100% identity with GenBank entries from Italy (Theileria sergenti), China (Theileria spp.), and Korea (Theileria buffeli isolate HS252). The results provide evidence for the presence of several hard tick species infesting cattle in Serbia which can carry pathogens potentially influencing animal health, as well as evidence of contact with tick-borne pathogens Theileria spp. and A. phagocytophilum.

Antimicrobial Resistance in Isolates From Sow Nares From Comercial Farms in Methicilllin Resistant Staphylococci (Mrs) And Expended Spectrum Beta Lactamate Antibiotics (Esbl) in Enterobacteriacea Models

Spreading of antimicrobial resistance is a great problem in human and animal medicine. Rising in occurrence in methicillinresistant Staphylococcus spp. (MRS) as well as expended spectrum beta lactamate antibiotics resistance (ESBL) in animals may be potential danger for farm workers in disease spread. Nasal and rectal swabs were taken from 53 sows without clinical symptoms on two farms in Serbia. Nasal swabs were tested on MRS and rectal on ESBL. Laboratory test showed 1 ESBL isolate (1.9%) and 18 (35.85%) MRS of which 3 (15.8%) Staphylococcus aureus and 7 (36.85%) Staphylococcus epidermidis. Seven of nineteen random MRS isolates were put on disc diffusion method. None of them showed resistance to vankomycine (VRSA) and fusidic acid.