Anabella Covazzi Harriague

Characteristics of the mesophotic megabenthic assemblages of the Vercelli Seamount (North Tyrrhenian Sea)

The biodiversity of the megabenthic assemblages of the mesophotic zone of a Tyrrhenian seamount (Vercelli Seamount) is described using Remotely Operated Vehicle (ROV) video imaging from 100 m depth to the top of the mount around 61 m depth. This pinnacle hosts a rich coralligenous community characterized by three different assemblages: (i) the top shows a dense covering of the kelp Laminaria rodriguezii; (ii) the southern side biocoenosis is mainly dominated by the octocorals Paramuricea clavata and Eunicella cavolinii; while (iii) the northern side of the seamount assemblage is colonized by active filter-feeding organisms such as sponges (sometimes covering 100% of the surface) with numerous colonies of the ascidian Diazona violacea, and the polychaete Sabella pavonina. This study highlights, also for a Mediterranean seamount, the potential role of an isolated rocky peak penetrating the euphotic zone, to work as an aggregating structure, hosting abundant benthic communities dominated by suspension feeders, whose distribution may vary in accordance to the geomorphology of the area and the different local hydrodynamic conditions.

Organic matter features, degradation and remineralisation at two coastal sites in the Ligurian Sea (NW Mediterranean) differently influenced by anthropogenic forcing

Organic matter (OM) features, degradation and remineralisation were studied in the seawater of a natural coastal site (Marine Protected Area of Portofino) and at an urbanised coastal site (Quarto, Genoa city) of the Ligurian Sea. The accumulation of low trophic value OM and the persistence of phytoplanktonic biomass throughout the year were observed only at the urbanised site. Efficient OM degradation and remineralisation via high-activity (two times higher than at the Portofino site) hydrolytic enzymatic activities (alkaline phosphatase and leucine aminopeptidase) were recorded. This active degradation indicated a generally good resistance of the Quarto system to environmental pressures (coastal inputs and anthropogenic influence). However, the high potential release of recycled inorganic nutrients and the favourable environmental conditions (i.e. higher seawater temperature at the Quarto site and allochthonous inputs), could encourage unpredictable development of the autotrophic fraction, including an already observed dystrophic blooming of toxic microalgae.

Organic matter recycling in a beach environment influenced by sunscreen products and increased inorganic nutrient supply (Sturla, Ligurian Sea, NW Mediterranean)

The beaches are sites where the human influence may be strong and the beach ecosystems have often shown a high sensibility to environmental alterations. These zones may be affected by a large series of anthropogenic-derived pressures, such as unbalanced inorganic nutrient input, that may cause anomalous development of primary production, altering the structure of the trophic webs. Furthermore, the utilisation of cosmetic sunscreen products is reaching unexpected levels, thus assuming a potentially important as well as unknown role in the contamination of marine environments. The present study was planned to test the response of the beach ecosystem to increases in inorganic nutrients (nitrate and phosphate) and to the input of a widely used cosmetic sunscreen product. A short-term laboratory experiment was carried out on microsystems consisting of sediments and seawater from the swash zone of a Ligurian city beach (Sturla). The processes related to organic matter (OM) recycling and some microbial food web components (bacteria and micro-autotrophic organisms) were analysed. The multivariate statistical analysis of the results showed that the increase in inorganic nutrients and sunscreen caused only a transient alteration in the OM recycling processes in the seawater. The sedimentary processes, instead, were different in the different systems, although starting from the same condition. In the sediment, surprisingly, an increase in inorganic nutrients did not lead to an increase in the primary biomass nor to significantly higher bacterial abundance, while the sunscreen caused increased OM recycling, especially devoted to protein and lipid mobilisation, supporting a growing bacterial and autotrophic community by reducing the bottom-up pressure. Additional toxicity tests performed on protozoa highlighted that, while the inorganic nutrients seemed to show no effects, sunscreen decreased the protozoan viability, thus likely favouring microautotrophic and bacterial increases by reducing the top-down pressure.

Organic matter characterisation and turnover in the sediment and seawater of a tourist harbour

A survey of a Ligurian tourist harbour was carried out during winter 2006 and summer 2007 in order to study the organic matter (OM) turnover through extracellular enzymatic activity. Seawater and sediments were sampled at six stations, three inside the port boundaries, one outside the port and two in an area influenced by the outflow of a minor river (Boate). The seawater showed OM turnover times similar to other oligo-mesotrophic coastal areas, and low concentrations of chlorophyll-a and inorganic nutrients. The sediments, instead, revealed high OM loads and a predominance of proteolysis. A significant reduction of the OM loads was observed in the outside station, indicating that the OM accumulation was due to the structures and activities of the harbour and to the Boate influence. The OM biotic recycling via enzymatic activity was enhanced especially during summer. Although the carbohydrates were probably highly refractory, their turnover was notably faster, due to glycolytic enzymatic activity that was enhanced more than the proteolytic in both the sediment and in the seawater. This suggested that the removal and recycling of OM were potentially efficient, and prevented the shift to eutrophication of the Rapallo harbour area.

Pelagic–benthic coupling in a subtidal system of the North-Western Mediterranean

Suspended particulate matter, zooplankton, and macrobenthos dynamics were investigated in a shallow area of the Ligurian Sea (north-west Mediterranean) characterized by wide temporal variability over an annual cycle. As indicated by multivariate analyses, the seasonal dynamics can be summarized as follows: (1) a late winter–early spring phytoplankton bloom followed by high zooplankton and macrobenthos densities during the spring months; (2) low-quality particulate suspended matter in summer, and an increase in the importance of zooplankton taxa with a wide range of feeding strategies, a decrease in macrofaunal abundance, and an increase in deposit-feeders and predators; and (3) a second phytoplankton bloom in autumn, followed by an increase in copepod density and a low macrofaunal abundance. In conclusion, pelagic and benthic communities in the coastal area of the Ligurian Sea mainly seem to be controlled bottom-up. Our results suggest that the quality of the particulate organic matter may play an important role in determining the temporal changes of both plankton and benthic assemblages, while the direct influence of other environmental features (such as sediment grain size) is relevant only for some macrobenthic taxa (e.g. crustaceans).

Linking Environmental Forcing and Trophic Supply to Benthic Communities in the Vercelli Seamount Area (Tyrrhenian Sea)

Seamounts and their influence on the surrounding environment are currently being extensively debated but, surprisingly, scant information is available for the Mediterranean area. Furthermore, although the deep Tyrrhenian Sea is characterised by a complex bottom morphology and peculiar hydrodynamic features, which would suggest a variable influence on the benthic domain, few studies have been carried out there, especially for soft-bottom macrofaunal assemblages. In order to fill this gap, the structure of the meio-and macrofaunal assemblages of the Vercelli Seamount and the surrounding deep area (northern Tyrrhenian Sea – western Mediterranean) were studied in relation to environmental features. Sediment was collected with a box-corer from the seamount summit and flanks and at two far-field sites in spring 2009, in order to analyse the metazoan communities, the sediment texture and the sedimentary organic matter. At the summit station, the heterogeneity of the habitat, the shallowness of the site and the higher trophic supply (water column phytopigments and macroalgal detritus, for instance) supported a very rich macrofaunal community, with high abundance, biomass and diversity. In fact, its trophic features resembled those observed in coastal environments next to seagrass meadows. At the flank and far-field stations, sediment heterogeneity and depth especially influenced the meiofaunal distribution. From a trophic point of view, the low content of the valuable sedimentary proteins that was found confirmed the general oligotrophy of the Tyrrhenian Sea, and exerted a limiting influence on the abundance and biomass of the assemblages. In this scenario, the rather refractory sedimentary carbohydrates became a food source for metazoans, which increased their abundance and biomass at the stations where the hydrolytic-enzyme-mediated turnover of carbohydrates was faster, highlighting high lability.

Intertidal benthic communities of two Chilean coastal islands (Santa María and Mocha, Southeastern Pacific)

Six macrotidal beaches located on two Chilean coastal islands (Santa María and Mocha, SE Pacific Ocean) were investigated in spring 2001 (29 October–3 November) to verify the role of the food supply (in terms of quantity and biochemical composition of the organic matter) on the abundance and diversity of the macro- and meiobenthic communities inhabiting the beaches. Samples of sediment were collected from the intertidal zone of the three beaches of each island investigated. The total organic matter content of the sediment did not differ between the islands, whereas the phytopigment and protein contents were significantly higher on Mocha (2.3±0.9 and 75±21 μ g g−1, mean values of the three beaches±SE, respectively) than on Santa María (0.5±0.2 and 50±16 μ g g−1, mean values of the three beaches±SE). The macro- and meiofaunal assemblages displayed the highest abundances on Mocha (821±223 and 561±194 ind. 10 cm−2, respectively). The abundance of both assemblages was correlated with the quality of the food supply, and significant correlations were observed with the phytopigment content (Spearman-rank R=0.645 p<0.01 and R=0.934 p<0.001 for the macro- and meiofauna, respectively). The results of the present study suggest that food availability may play a key role in structuring benthic communities of the oceanic beaches of Chilean coastal islands. A single observation is not enough to fully understand the real mechanisms that shape the beach communities, however, the snapshot image we collected during this study suggests that the role of food availability in shaping benthic beach communities may be as important as that played by the hydrodynamic conditions.

Macro- and meiofaunal community features in the critical environmental system of a tourist harbour (Rapallo, Ligurian Sea, NW Mediterranean).

Two samplings were carried out in a tourist harbour, during low and high touristic activity periods, to study the macro- and meiofaunal communities in relation to the environmental features. A multivariate analysis showed close relationships: the maritime traffic disturbance and the food quality and availability drive the spatial differences of the assemblages, dividing the area into three sub-areas: the area near the Boate torrent that empties into the harbour, the harbour proper, and the external area (just outside the harbour). Macro- and meiofauna showed notably different temporal trends, indicating competition for the resources and the higher sensitivity of the macrofauna to environmental pressures. The macrofauna strongly decreased as a response to heavier harbour activities, with increasing turbidity also affecting the external station outside the harbour. Finally, comparing the macrofaunal communities to those sampled in the same area 10 years before, we found that their abundance, richness and biomass had notably decreased, highlighting the worsening of the harbour environment due to the increased organic load and turbidity.

Urbanised beaches of the Ligurian coastal area (NW Mediterranean): A classification based on organic-matter characteristics and hydrolytic enzymatic activities

The beaches of Liguria have been intensively affected by human activities for over a century, transforming nearly the entire coastline from natural to urbanised and significantly upsetting beach ecological properties. The present study aims to investigate 9 Ligurian beaches characterised by different degree of urbanisation, to test if and to what extent the organic-matter (OM) recycling processes can be linked to the human activity. Swash zone sediment, sampled during the spring-summer-autumn period, when the anthropogenic influence is at its maximum due to tourism, was analysed for OM features and recycling processes. Multivariate statistical analyses showed that huge amounts of detrital OM accumulated in the more urbanised sites, where the anthropogenic influence was at its peak, deriving from higher inhabitant number and density, from the presence of crowded roads very near to the swash zone and sewage treatment plants. The presence of torrent outlets on the beaches provided further OM accumulation. Lipids, carbohydrates and degraded autotrophic pigments were the OM fractions mainly responsible of the differentiation, and rather constant, high labile phosphorus contents were found in the more urbanised sites. The high activity values of the hydrolytic enzymes indicate the response of the microbial system to the OM accumulation in the urban sites. However, a decoupling of the trends of some enzymatic activities (namely glucosidase and lipase) and their target OM was observed in the highly urbanised conditions.