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Dive into the research topics where Anamaria Rekecki is active.

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Featured researches published by Anamaria Rekecki.


Environmental Microbiology | 2009

Development of a bacterial challenge test for gnotobiotic sea bass (Dicentrarchus labrax) larvae.

Kristof Dierckens; Anamaria Rekecki; Stanislas Laureau; Patrick Sorgeloos; Nico Boon; W. Van den Broeck; Peter Bossier

The use of probiotic microorganisms in aquaculture is gaining a lot of interest. Gnotobiotic model systems are required in order to fully understand the effects and modes-of-action of these microorganisms, as the native microbial communities present in non-sterile animals can lead to false conclusions. In this study, a gnotobiotic sea bass larvae (Dicentrarchus labrax) test system was developed. In order to obtain bacteria-free animals, the eggs were disinfected with glutaraldehyde and subsequently incubated in a solution of rifampicin and ampicillin. Axenity was confirmed using culture-dependent and -independent techniques. The gnotobiotic larvae were fed axenic Artemia sp. from 7 days after hatching onwards. In the challenge test, one of the three opportunistic pathogens, Aeromonas hydrophila, Listonella anguillarum serovar O1 and O2a, was added to the model system via the water and encapsulated in Artemia sp. Only serovar O2a led to increased mortality in the sea bass larvae. The presented gnotobiotic model can be used for research on, among others, reciprocal metabolic effects between microorganisms and the host (e.g. as measured by gene expression), immunostimulants, pharmacological research and the histological development of the gastrointestinal tract and growth of larvae.


PLOS ONE | 2011

Herpes Simplex Virus Type 1 Penetrates the Basement Membrane in Human Nasal Respiratory Mucosa

Sarah Glorieux; Claus Bachert; Herman Favoreel; Annelies Vandekerckhove; Lennert Steukers; Anamaria Rekecki; Wim Van Den Broeck; Joline Goossens; Siska Croubels; Reginald Clayton; Hans Nauwynck

Background Herpes simplex virus infections are highly prevalent in humans. However, the current therapeutics suffer important drawbacks such as limited results in neonates, increasing occurrence of resistance and impeded treatment of stromal infections. Remarkably, interactions of herpesviruses with human mucosa, the locus of infection, remain poorly understood and the underlying mechanisms in stromal infection remain controversial. Methodology/Principal Findings A human model consisting of nasal respiratory mucosa explants was characterised. Viability and integrity were examined during 96 h of cultivation. HSV1-mucosa interactions were analysed. In particular, we investigated whether HSV1 is able to reach the stroma. Explant viability and integrity remained preserved. HSV1 induced rounding up and loosening of epithelial cells with very few apoptotic and necrotic cells observed. Following 16–24 h of infection, HSV1 penetrated the basement membrane and replicated in the underlying lamina propria. Conclusions/Significance This human explant model can be used to study virus-mucosa interactions and viral mucosal invasion mechanisms. Using this model, our results provide a novel insight into the HSV1 stromal invasion mechanism and for the first time directly demonstrate that HSV1 can penetrate the basement membrane.


Laboratory Animals | 2010

Surface area assessment of the murine intestinal tract as a prerequisite for oral dose translation from mouse to man

Christophe Casteleyn; Anamaria Rekecki; A Van der Aa; Paul Simoens; W. Van den Broeck

In many pharmacological and toxicological studies knowledge about the intestinal absorption, which is dependent upon the surface area of absorptive epithelia, is indispensible. Although mice are often used in such preclinical studies, very few quantitative data about their intestinal surface area are available. Especially for locally acting candidate drugs in development, this information is crucial for dose translation towards humans. Therefore, the surface area of the intestinal tract of CD-1™ IGS mice was assessed in the present study. The intestinal tracts of 12 mice were collected after euthanasia. From six animals, histological sections from the duodenum, jejunum, ileum, caecum and colon-rectum were made according to common stereological principles. Using these sections, the volumes and surface areas of each intestinal segment were estimated applying stereological counting procedures. In the other six animals, the density and surface area of the microvilli present in each intestinal segment were determined by means of scanning and transmission electron microscopy to assess the increase of the intestinal surface area attributable to the presence of microvilli. The mean total volume and surface area of the intestinal tract were 1.34 cm3 and 1.41 m2, respectively. The relative intestinal surface area (intestinal surface area divided by the body surface area) was 119. The relative intestinal surface area of mice is very similar to that of humans. The results of this study are important for the appropriate dose translation of candidate therapeutic compounds in drug development from mouse to humans.


Journal of Fish Diseases | 2012

Bacterial host interaction of GFP-labelled Vibrio anguillarum HI-610 with gnotobiotic sea bass, Dicentrarchus labrax (L.), larvae

Anamaria Rekecki; Raysa Gunasekara; Kristof Dierckens; Stanislas Laureau; Nico Boon; Herman Favoreel; Maria Cornelissen; Patrick Sorgeloos; Richard Ducatelle; Peter Bossier; W. Van den Broeck

The location and cell damage caused by Vibrio anguillarum, the causative agent of classical vibriosis, within the developing gut of the newly hatched sea bass, Dicentrarchus labrax (L.), is unknown. A gnotobiotic sea bass model was used to investigate the early interactions of V. anguillarum with sea bass larvae. In the present study, germ-free sea bass larvae were orally exposed to a V. anguillarum HI-610 pathogen labelled with the green fluorescent protein (GFP-HI-610) and sampled at regular intervals. Pathogenic colonization of gut enterocytes was observed 2 h post-exposure (p.e.) and onwards, whereas bacteria within the swim bladder were visualized 48 h p.e and onwards. Ultrastructural findings demonstrated direct bacterial contact with the host cell in the oesophageal mucosa and putative attachment to microvilli of mid- and hindgut enterocytes. The present findings form a starting point for studies assessing the impact of potential candidates (probiotics, prebiotics, antimicrobial peptides) to mitigate bacterial virulence.


Journal of Fish Diseases | 2014

White necrotic tail tips in estuary seahorses, Hippocampus kuda, Bleeker

Annelies Declercq; Koen Chiers; W. Van den Broeck; Anamaria Rekecki; S Teerlinck; Dominique Adriaens; Freddy Haesebrouck; Annemie Decostere

1 Department of Morphology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium 2 Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium 3 INVE Technologies, Dendermonde, Belgium 4 Veterinarian for Fish, Private Practice, Gavere, Belgium 5 Research Group Evolutionary Morphology of Vertebrates, Faculty of Sciences, Ghent University, Ghent, Belgium


Veterinary Microbiology | 2012

Light and transmission electron microscopy of Vibrio campbellii infection in gnotobiotic Artemia franciscana and protection offered by a yeast mutant with elevated cell wall glucan

R.A.Y.S. Asanka Gunasekara; Tom Defoirdt; Anamaria Rekecki; Annemie Decostere; Maria Cornelissen; Patrick Sorgeloos; Peter Bossier; Wim Van Den Broeck

Luminescent vibrios are amongst the most important pathogens in aquaculture, affecting almost all types of cultured organisms. Vibrio campbellii is one of these most important pathogens. In this study, the effects of feeding mnn9 yeast cell wall mutant and wild type yeast strain were investigated in the digestive tract of brine shrimp nauplii, Artemia franciscana, after experimental infection with V. campbellii (LMG 21363). Gnotobiotic A. franciscana nauplii were fed daily with dead Aeromonas hydrophila LVS3, and with either wild type strain of bakers yeast, Saccharomyces cerevisiae, or mutant strain mnn9, of which the cell wall contains elevated chitin and glucan and lower mannose levels. After three days of feeding, some nauplii were challenged with V. campbellii. Mean survival (%), individual length (mm) and total length (mm) at one day and two days after challenge were significantly higher in the group fed mnn9 than in the group fed wild type yeast (81 ± 1.50 and 63 ± 0.49, 1.56 ± 0.07 and 1.13 ± 0.02, 38.21 ± 3.11 and 21.26 ± 0.81 respectively for one day and 50 ± 2.37 and 20 ± 1.41, 2.33 ± 0.01 and 1.24 ± 0.04, 34.97 ± 5.56 and 7.45 ± 1.63 for two days after challenge). Histological examination revealed that the luminal diameter and enterocyte height of both mid- and hindgut were larger in the mnn9-fed group. Colonization of the gut lumen by V. campbellii could be observed by transmission electron microscopy for the group of nauplii fed with wild type yeast. Furthermore, it was observed that V. campbellii caused damage to the gut epithelium including shortening and disappearance of the microvilli, destruction of the apical cell membrane and cell lysis in the nauplii fed wild type yeast. The gut epithelium remained intact in challenged nauplii fed mnn9 yeast. The morphological findings of the present study further substantiate previous studies reporting a protective effect of this yeast cell wall mutant.


Journal of Microbiological Methods | 2012

A method for the specific detection of resident bacteria in brine shrimp larvae

Yufeng Niu; Tom Defoirdt; Anamaria Rekecki; Peter De Schryver; Wim Van Den Broeck; Shuanglin Dong; Patrick Sorgeloos; Nico Boon; Peter Bossier

In this study, we describe an easy but efficient method to specifically target the intestinal resident microbiota in brine shrimp larvae during DGGE analysis, hereby excluding the interference of both transient (luminal) bacteria and body surface bacteria. This effective technique has several advantages over alternative methods, with respect of ease of use and rapidity.


Aquaculture Research | 2015

Siberian sturgeon (Acipenser baerii) larvae fed Artemia nauplii enriched with poly-β-hydroxybutyrate (PHB) : effect on growth performance, body composition, digestive enzymes, gut microbial community, gut histology and stress tests

Ebrahim H. Najdegerami; Kartik Baruah; Alireza Shiri; Anamaria Rekecki; Wim Van Den Broeck; Patrick Sorgeloos; Nico Boon; Peter Bossier; Peter De Schryver


Journal of Experimental Marine Biology and Ecology | 2010

Evaluation of probiotic effect of Aeromonas hydrophila on the development of the digestive tract of germ-free Artemia franciscana nauplii

R.A.Y.S. Asanka Gunasekara; Anamaria Rekecki; Kartik Baruah; Peter Bossier; Wim Van Den Broeck


Journal of Fish Diseases | 2013

Luminal uptake of Vibrio (Listonella) anguillarum by shed enterocytes – a novel early defence strategy in larval fish

Anamaria Rekecki; Einar Ringø; Randi Olsen; Reidar Myklebust; Kristof Dierckens; Øivind Bergh; Stanislas Laureau; Maria Cornelissen; Richard Ducatelle; Annemie Decostere; Peter Bossier; W. Van den Broeck

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