Ananda B. W. Manage

Vertical migration of Haemonchus contortus infective larvae on Cynodon dactylon and Paspalum notatum pastures in response to climatic conditions.

Observations were made on vertical migration patterns of Haemonchus contortus infective larvae on Cynodon dactylon (bermudagrass) and Paspalum notatum (bahiagrass) pastures under summer climatic conditions typical of East Texas. Ten thousand H. contortus infective larvae (L3) were introduced to 100 cm(2) subplots of each pasture species within a plot area of 1m(2). Subplots were inoculated with larvae by applying them in an aqueous medium to the soil or mat beneath the vegetation. Herbage from the inoculated areas was harvested on 5 sampling days over a span of 21 days. L3 recoveries were observed and recorded each day on four herbage strata viz. 0-5, 5-10, 10-20 and >20 cm from ground level. The log transformed larval recovery data were analyzed for effect of day, stratum, and day x stratum interaction for each grass species during two separate experimental periods. Precipitation, relative humidity and temperature during the study were subjected to correlation and multiple regression analyses with the larval counts. Significant (P</=0.0017) differences were found for the effect of day on larval recoveries. No significant differences were detected for stratum or day x stratum interaction effects, though stratum provided a strong indication of influencing larval recovery. A high positive correlation (>or=0.93) between rainfall and total average daily larval counts was apparent. The multiple regression analysis did not show significant results for any of the climatic factors tested. This study showed that the H. contortus infective larvae can survive beyond 21 days in the soil and infest pasture grasses when the climatic conditions are favorable. Avoiding use of H. contortus contaminated pasturelands in summer at the onset of rainfall following a dry spell may effectively reduce nematode loads in susceptible farm animals. Additional studies should focus on factors affecting long term L3 survivability, migrational pattern on these and other plant species and the relationship between climatic factors and larval migration patterns throughout the year. Total larval recovery of H. contortus in this study was greater in bahiagrass than bermudagrass. While the design of this study did not allow for testing one pasture species against another, studies with potted plants would allow for some valid comparisons. Soil characteristics may also play a role in L3 survival and subsequent migration.

Nano-intercalated rhodanese in cyanide antagonism

Abstract Present studies have focused on nano-intercalated rhodanese in combination with sulfur donors to prevent cyanide lethality in a prophylactic mice model for future development of an effective cyanide antidotal system. Our approach is based on the idea of converting cyanide to the less toxic thiocyanate before it reaches the target organs by utilizing sulfurtransferases (e.g., rhodanese) and sulfur donors in a close proximity by injecting them directly into the blood stream. The inorganic thiosulfate (TS) and the garlic component diallydisulfide (DADS) were compared as sulfur donors with the nano-intercalated rhodanese in vitro and in vivo. The in vivo and in vitro experiments showed that DADS is not a more efficient sulfur donor than TS. However, the utilization of external rhodanese significantly enhanced the in vivo efficacy of both sulfur donor-nitrite combinations, indicating the potential usefulness of enzyme nano-delivery systems in developing antidotal therapeutic agents.

A study of the powerplay in one-day cricket

This paper investigates the powerplay in one-day cricket. The rules concerning the powerplay have been tinkered with over the years, and therefore the primary motivation of the paper is the assessment of the impact of the powerplay with respect to scoring. The form of the analysis takes a “what if” approach where powerplay outcomes are substituted with what might have happened had there been no powerplay. This leads to a paired comparisons setting consisting of actual matches and hypothetical parallel matches where outcomes are imputed during the powerplay period. Some of our findings include (a) the various forms of the powerplay which have been adopted over the years have different effects, (b) recent versions of the powerplay provide an advantage to the batting side, (c) more wickets also occur during the powerplay than had there been no powerplay and (d) there is some effect in run production due to the over where the powerplay is initiated. We also investigate individual batsmen and bowlers and their performances during the powerplay.

The host effects of Gambusia affinis with an antibiotic-disrupted microbiome.

While serving as critical tools against bacterial infections, antimicrobial therapies can also result in serious side effects, such as antibiotic-associated entercolitis. Recent studies utilizing next generation sequencing to generate community 16S gene profiles have shown that antibiotics can strongly alter community composition and deplete diversity. However, how these community changes in the microbiota are related to the host side effects is still unclear. We have used the freshwater Western mosquitofish (Gambusia affinis) as a tractable vertebrate model system to study host effects following exposure to a broad spectrum antibiotic, rifampicin. After 3days of exposure, the bacterial communities of the mucosal skin and gut microbiomes lost diversity and shifted composition. Compared to unexposed controls, treated fish were more susceptible to a specific pathogen, Edwardsiella ictaluri, yet displayed no survival differences when subjected to a polymicrobial water challenge of soil or feces. Treated fish were more susceptible to osmotic stress from NaCl, but not to the toxin nitrate. Treated fish failed to gain weight as well as controls over one month when fed a matched diet. Because of small sample sizes, pathogen susceptibility and weight gain differences were not statistically significant. This study provides supporting evidence in an experimental laboratory system that an antibiotic can have significant and persistent negative host effects, and provides for future study into the mechanisms of these effects.

An Introductory Application of Principal Components to Cricket Data.

Principal Component Analysis is widely used in applied multivariate data analysis, and this article shows how to motivate student interest in this topic using cricket sports data. Here, principal component analysis is successfully used to rank the cricket batsmen and bowlers who played in the 2012 Indian Premier League (IPL) competition. In particular, the first principal component is seen to explain a substantial portion of the variation in a linear combination of some commonly used measures of cricket prowess. This application provides an excellent, elementary introduction to the topic of principal component analysis.

Improved tests for homogeneity of variances

ABSTRACT Equality of variances is one of the key assumptions of analysis of variances (ANOVA). There are several testing procedures available to validate this assumption, but it is rare to find a test procedure which controls the type I error rate while providing high statistical power. In this article, we introduce a bootstrap test based on the ratio of mean absolute deviances (RMD). We also propose a two-stage testing procedure where we first quantify the skewness of the distributions and then choose an appropriate test for homogeneity of variances. The performances of these test procedures are studied via a simulation study.

How Well Do Multiple Testing Methods Scale Up when Both n and k Increase

With increasingly massive data sets in biopharmaceutical research, particularly in genomic and related applications, there is concern about how well multiple comparisons methods “scale up” with increasing number of tests (k). Familywise error rate-controlling methods do not scale up well, and false discovery rate-controlling methods do scale up well with increasing k. But neither method scales up well with increasing sample size (n) when testing point nulls. We develop a loss function approach to investigate scale-up properties of various methods; we find that while Efrons recent proposal scales up best when both sample size n and number of tests k increase, but its performance otherwise can be erratic.

Attendance: Case-Study in Developmental Mathematics Classrooms

Increasing classroom attendance rates is important to improving success in developmental mathematics courses. Our results indicated that absence rates begin to increase after the first exam. Furthermore, the number of absences gradually increased throughout the semester and a higher proportion of class meeting time was missed in classes that met three days per week compared to two days per week. Thus, attempts to emphasize to students the importance of classroom attendance on course grades needs to begin before the first test. When absences were measured by proportion of class meeting time, effect of absences on students’ failing the course was higher for classes that met two days per week compared to classes that met three days per week.

Confidence limit calculation for antidotal potency ratio derived from lethal dose 50.

AIM To describe confidence interval calculation for antidotal potency ratios using bootstrap method. METHODS We can easily adapt the nonparametric bootstrap method which was invented by Efron to construct confidence intervals in such situations like this. The bootstrap method is a resampling method in which the bootstrap samples are obtained by resampling from the original sample. RESULTS The described confidence interval calculation using bootstrap method does not require the sampling distribution antidotal potency ratio. This can serve as a substantial help for toxicologists, who are directed to employ the Dixon up-and-down method with the application of lower number of animals to determine lethal dose 50 values for characterizing the investigated toxic molecules and eventually for characterizing the antidotal protections by the test antidotal systems. CONCLUSION The described method can serve as a useful tool in various other applications. Simplicity of the method makes it easier to do the calculation using most of the programming software packages.