Anatoly Masalov

Effect of iron oxide nanoparticles on the blood coagulation according to light scattering data

The work shows that iron oxide nanoparticles obtained in acoustoplasma discharge with cavitation affect the rate of one of the reactions of the process of blood coagulation - cleavage of fibrinogen by thrombin. As a result of the reaction, a fibrin gel is formed. By means of dynamic and static light scattering we reveal that adding of thrombin initially mixed with nanoparticles to the fibrinogen solution leads to dramatically acceleration of gel formation. Adding of nanoparticles to the solution of fibrinogen (before thrombin addition) leads to stopping of the reaction at the first stage (without gel formation). This data shows that ferrous oxide nanoparticles can act as regulators of enzyme reaction - in one case accelerating it, and in the other - by inhibiting it. Previously we presented the dynamics of distributions of the scattered light intensity on particle sizes in the fibrinogen-thrombin system with various sequences of nanoparticle addition. In this work we showed dynamics of the intensity correlation function in the samples, whose form becomes close to “stretched exponent” in pregel state or power law in a gel.

Effect of iron oxide nanoparticles on the concentration-versus-sizes relation of proteins in the blood plasma and serum, and in model solutions

The effect of iron (III) oxide nanoparticles produced in acoustoplasma discharge with cavitation on the concentration and the sizes of particles in model protein solutions, human blood serum and plasma samples is studied. Dynamic and static light scattering data on size and concentration of particles show that the nanoparticles addition to fibrinogen-thrombin system affects the course of enzymatic reaction. Interaction of nanoparticles with fibrinogen solution (before thrombin addition) does not significantly change the distribution of scattered light intensity on particle sizes. Comparison of the relations of particle sizes and their concentration for fibrinogen solution with and without nanoparticles shows an increase of the slope of size-concentration relation in a log-log scale, which indicates an increase in the concentration of small particles and decrease of big ones. For model solution of fibrinogen with thrombin, initially incubated with iron oxide nanoparticles, the slopes of the size-concentration relation equals to k = –(4.62±0.33) and slightly differs from the slope of the relation for fibrinogen-thrombin system without nanoparticles k=–(4.23±0.28). We believe that changes in the size-concentration relation indicate the interaction of nanoparticles with proteins, which results in gelation rate change.