André L. Teixeira

Influência das diferentes fases do ciclo menstrual na flexibilidade de mulheres jovens

Introduction: The menstrual cycle is the main responsible for changes in female physiology, which may affect some morphofunctional responses. Objective: to investigate the influence of the different phases of the menstrual cycle on the physical flexibility of young women. Methods: 44 volunteers were divided into a control group (n = 24), which made regular use of hormonal contraceptives, and an experimental group (n = 20), which did not use contraceptives. All volunteers underwent three days of evaluations, one for each phase of menstrual cycle (follicular, ovulatory and luteal). Anthropometric data (body mass, body mass index, waist and abdomen circumferences), and body composition data (body fat percentage and lean mass) were assessed. Flexibility was then analyzed through the sit and reach test on Wells bench. The non-parametric Mann-Whitney test was then applied for intragroup comparisons, and the Friedman test for comparison between the different menstrual phases. Results: No significant differences between groups within and between different phases of the cycle were observed (p > 0.05). Greater variability within the control group was observed when compared to the experimental group. Conclusion: Regardless of the menstrual cycle phase and of the use of hormonal contraceptives, the physical flexibility is not altered in young women.

Relação entre o nível de atividade física e a incidência da síndrome pré-menstrual

PURPOSE To determine the relationship between the level of physical activity and the incidence of premenstrual syndrome. METHODS A cross-sectional design was conducted on 71 apparently healthy university students (24.4±4.8 yrs; 61.5±8.7 kg; 1.63±0.06 m). The level of physical activity was determined with a questionnaire and the presence of premenstrual syndrome was verified based on daily symptoms self-reported in a diary during two consecutive menstrual cycles. 17 premenstrual symptoms are considered in the diary, which should be scored on a 5-point scale (0-4) according to their occurrence, so that a score can be calculated in each cycle. The occurrence of premenstrual syndrome was considered if three or more symptoms were reported up to six days before menstruation (premenstrual period) and were absent up to six days after menstruation (postmenstrual period). RESULTS The Spearman correlation coefficient showed a significant and negative relationship between the level of physical activity and premenstrual syndrome score (r=-0.506; 95%CI -0.335 to -0.678; p<0.001). When the participants were divided into a group with a positive diagnosis of premenstrual syndrome (n=31) and a healthy group (n=40), the Mann-Whitney test showed higher habitual physical activity in the healthy group than in the premenstrual syndrome group (7.96±1.17 and 6.63±1.20, respectively) (p<0.001). CONCLUSIONS There is a negative relationship between the level of physical activity and the incidence of premenstrual syndrome, with women with a positive diagnosis of premenstrual syndrome having a lower level of physical activity than healthy women.

Heart rate variability across the menstrual cycle in young women taking oral contraceptives

Previous studies have shown that resting heart rate variability (HRV) is modified by different phases of the menstrual cycle in nonusers of oral contraceptive pills (OCP); however, the effect of OCP on autonomic control of the heart remains unclear. The purpose of this study was to investigate HRV during the low hormone (LH-not taking OCP) and during the high hormone (HH-active OCP use) phases of the menstrual cycle in young women. Seventeen healthy women (19-31 years) taking OCP for at least 6 consecutive months were enrolled in this study. Plasma estradiol and progesterone were verified at each visit. HRV was assessed by using one-lead electrocardiography in time and frequency domains, in which participants rested in the supine position for a 20-min period with a breathing rate of 15 cycles/min. In addition, resting heart rate, and systolic and diastolic blood pressure were obtained. Both plasma estradiol (LH: 19.8 ± 4.2 pg/mL vs. HH: 12.4 ± 1.5 pg/mL; p > .05) and progesterone (LH: 0.247 ± 0.58 ng/mL vs. HH: 0.371 ± 0.08 ng/mL; p > .05) (mean ± SE) levels were similar in both phases. No significant difference was obtained for any component of HRV, heart rate, or blood pressure between the LH and HH phases (p > .05). These results provide preliminary evidence that use of OCP does not affect HRV during the menstrual cycle in healthy women.

GABAergic contribution to the muscle mechanoreflex-mediated heart rate responses at the onset of exercise in humans

Previous studies have indicated that central GABAergic mechanisms are involved in the heart rate (HR) responses at the onset of exercise. On the basis of previous research that showed similar increases in HR during passive and active cycling, we reasoned that the GABAergic mechanisms involved in the HR responses at the exercise onset are primarily mediated by muscle mechanoreceptor afferents. Therefore, in this study, we sought to determine whether central GABA mechanisms are involved in the muscle mechanoreflex-mediated HR responses at the onset of exercise in humans. Twenty-eight healthy subjects (14 men and 14 women) aged between 18 and 35 yr randomly performed three bouts of 5-s passive and active cycling under placebo and after oral administration of diazepam (10 mg), a benzodiazepine that produces an enhancement in GABAA activity. Beat-to-beat HR (electrocardiography) and arterial blood pressure (finger photopletysmography) were continuously measured. Electromyography of the vastus lateralis was obtained to confirm no electrical activity during passive trials. HR increased from rest under placebo and further increased after administration of diazepam in both passive (change: 12 ± 1 vs. 17 ± 1 beats/min, P < 0.01) and active (change: 14 ± 1 vs. 18 ± 1 beats/min, P < 0.01) cycling. Arterial blood pressure increased from rest similarly during all conditions ( P > 0.05). Importantly, no sex-related differences were found in any variables during experiments. These findings demonstrate, for the first time, that the GABAergic mechanisms significantly contribute to the muscle mechanoreflex-mediated HR responses at the onset of exercise in humans. NEW & NOTEWORTHY We found that passive and voluntary cycling evokes similar increases in heart rate and that these responses were enhanced after diazepam administration, a benzodiazepine that enhances GABAA activity. These findings suggest that the GABAergic system may contribute to the muscle mechanoreflex-mediated vagal withdrawal at the onset of exercise in humans.

Impaired popliteal artery flow-mediated dilation caused by reduced daily physical activity is prevented by increased shear stress

We recently showed that 5 days of reduced daily physical activity impair popliteal artery, but not brachial artery, flow-mediated dilation (FMD). However, the mechanisms by which physical inactivity causes leg vascular dysfunction are unclear. We reason that a reduction in leg blood flow-induced shear stress is a primary underlying mechanism by which reduced daily physical activity impairs popliteal artery FMD. Thus the purpose of this study was to determine whether increased leg blood flow and shear stress during inactivity prevent the reduction in popliteal artery FMD. Bilateral popliteal artery FMD measures were performed at baseline and after 5 days of a transition from high (>10,000 steps/day) to low levels (<5,000 steps/day) of physical activity in 13 healthy and physically active men [20 ± 2 (SD) yr]. During the inactive period, one foot was submerged in ~42°C water (i.e., heated leg) three times a day for 30 min each period, to increase blood flow and thus shear stress, whereas the contralateral leg remained dry and served as internal control (i.e., nonheated leg). During heating, popliteal artery mean shear rate was increased in the heated leg (change of 119.3 ± 26.4%, P < 0.01) but slightly decreased in the nonheated leg (change of -21.8 ± 7.5%, P = 0.03). Popliteal artery FMD was impaired after 5 days of reduced daily physical activity in the control nonheated leg (P < 0.01) but was unchanged in the heated leg (P = 0.34). These results support the hypothesis that reduced leg blood flow-induced shear stress during physical inactivity is a key underlying mechanism mediating leg vascular dysfunction.NEW & NOTEWORTHY We found that the impairment in popliteal artery flow-mediated dilation caused by physical inactivity can be prevented by increased shear stress. These findings indicate that reduced leg blood flow-induced shear stress during physical inactivity may be a key underlying mechanism mediating the detrimental leg vascular effects of physical inactivity. Heating the foot area may be used as a nonpharmacological therapy to combat inactivity-induced leg vascular dysfunction, especially in people who are unable or unwilling to be active.

Effects of ovarian hormones and oral contraceptive pills on cardiac vagal withdrawal at the onset of dynamic exercise.

The purpose of this study was to investigate the effects of the ovarian hormones and the use of oral contraceptive pills (OCP) on cardiac vagal withdrawal at the onset of dynamic exercise. Thirty physically active women aged 19–32 years were divided into two groups: OCP users (n = 17) and non-OCP users (n = 13). Participants were studied randomly at three different phases of the menstrual cycle: early follicular (day 3.6 ± 1.2; range 1–5), ovulatory (day 14.3 ± 0.8; range 13–16) and midluteal (day 21.3 ± 0.8; range 20–24), according to endogenous (in non-OCP users) or exogenous (in OCP users) estradiol and progesterone variations. The cardiac vagal withdrawal was represented by the cardiac vagal index (CVI), which was obtained by the 4-s exercise test. Additionally, resting heart rate, systolic (SBP) and diastolic blood pressure (DBP) were obtained. The CVI was not significantly different between the three phases of the menstrual cycle in either the non-OCP users (early follicular: 1.58 ± 0.1; ovulatory: 1.56 ± 0.1; midluteal: 1.58 ± 0.1, P > 0.05) or OCP users (early follicular: 1.47 ± 0.1; ovulatory: 1.49 ± 0.1; midluteal: 1.47 ± 0.1, P > 0.05) (mean ± SEM). Resting cardiovascular responses were not affected by hormonal phase or OCP use, except that the SBP was higher in the OCP users than non-OCP users in all phases of the cycle (P < 0.05). In summary, our results demonstrate that cardiac vagal withdrawal at the onset of dynamic exercise was not impacted by the menstrual cycle or OCP use in physically active women.

Capsaicin-based analgesic balm attenuates the skeletal muscle metaboreflex in healthy humans

The exercise pressor reflex (EPR) is comprised of group III and IV skeletal muscle afferents and is one of the principal mediators of the cardiovascular response to exercise. In animals, capsaicin-based analgesic balm (CAP) attenuates the pressor response to muscle contraction, indicating the transient receptor potential vanilloid 1 (TRPv1) receptor (localized on the group IV afferent neuron) as an important mediator of the EPR. However, whether these findings can be extrapolated to humans remains unknown. Here, we tested the hypothesis that CAP would attenuate blood pressure (BP) and muscle sympathetic nerve activity (MSNA) responses to isolated muscle metaboreflex activation in healthy men. MSNA (microneurography) and beat-to-beat heart hate (HR, by electrocardiography), and BP (finger photoplethysmography) were continuously measured in eight healthy males (23 ± 5 yr) at rest, during isometric handgrip exercise, and during postexercise ischemia (PEI). Trials were performed before and 30 and 60 min after the topical application of CAP (0.1%, CAPZASIN-HP) over the volar forearm of the subjects exercising arm. Isometric exercise evoked increases in mean BP (∆32 ± 4 mmHg) and MSNA (∆26 ± 5 bursts/min; ∆19 ± 5 bursts/100 heart beats). The increases in BP during handgrip were not affected by CAP, but the increase in MSNA was lower after 60 min of CAP application. During PEI, the increases in BP and MSNA were all significantly less than those before CAP (all P < 0.05). In conclusion, CAP attenuated BP and sympathetic responses evoked by PEI in humans. These data provide evidence that transient receptor potential vanilloid 1 receptors potentially contribute to the EPR in humans, via its metabolic component. NEW & NOTEWORTHY We found that topical application of capsaicin-based analgesic balm attenuates arterial blood pressure and muscle sympathetic nerve activity during isolated muscle metaboreflex activation following isometric handgrip exercise in healthy humans. These findings suggest that the transient receptor potential vanilloid 1 may contribute to the exercise pressor reflex in humans via its metabolic component.

Sympathetically-mediated cardiac responses to isolated muscle metaboreflex activation following exercise are modulated by body position in humans

Isolated muscle metaboreflex activation with posthandgrip exercise ischemia (PEI) increases sympathetic nerve activity and partially maintains the exercise-induced increase in blood pressure, but a smaller heart rate (HR) response occurs. The cardiopulmonary baroreceptors, mechanically sensitive receptors that respond to changes in central blood volume and pressure, are strongly associated with changes in body position and upon activation elicit reflex sympathoinhibition. Here, we tested the hypothesis that postural changes modulate the sympathetically mediated cardiac response to PEI in humans. Beat-to-beat HR (electrocardiography) and blood pressure (finger photoplethysmography) were continuously measured, and cardiac function was assessed by echocardiography in 13 healthy men (21 ± 3 yr). After a 15-min rest period, 90-s static handgrip at 40% maximum voluntary contraction was performed followed by 3 min of PEI. Four trials were randomly conducted during either seated or supine position with and without β1-adrenergic blockade (25 mg atenolol). During PEI under control conditions, HR remained elevated from baseline in the seated [change (Δ): 4 ± 1 beats/min] but not in the supine (change: -1 ± 1 beats/min) position. Similarly, stroke volume and cardiac output were increased from baseline in the seated (∆13.0 ± 2.4 ml and ∆1.1 ± 0.2 l/min, respectively) but not in the supine (∆2.5 ± 2.9 ml and ∆0.13 ± 0.20 l/min, respectively) position. During β1-adrenergic blockade, HR, stroke volume, and cardiac output remained unchanged in both conditions. We conclude that sympathetically mediated cardiac responses to PEI are influenced by changes in body position. These findings indicated that muscle metaboreflex and cardiopulmonary baroreflex have an interactive influence on the neural control of cardiovascular function in humans. NEW & NOTEWORTHY In the present study, we demonstrated that muscle metaboreflex activation increases heart rate, stroke volume, and cardiac output in the seated position but not in the supine position and not after β1-adrenergic blockade. These findings indicate that sympathetically mediated cardiac responses to isolated muscle metaboreflex activation after exercise are modulated by central blood volume mobilization.

The role of water intake on cardiac vagal reactivation after upper-body resistance exercise.

The aim of this study was to assess the hypothesis that water intake will accelerate cardiac vagal reactivation after a single session of upper-body resistance exercise. 13 healthy men (26.5±5.9 years) with previous experience in resistance training were enrolled. In visits 1 and 2, participants performed the one-repetition maximum (1RM) test and retest with the bench press exercise. The sessions 3 and 4 were performed randomly, while participants consumed 500 ml (experimental visit) or 50 ml (control visit) of water immediately after 3 sets of maximum repetitions at 80% of 1RM. Cardiac vagal activity was represented by cardiac vagal index (CVI) measured before, immediately after and 30 min post-exercise. Additionally, heart rate and blood pressure were measured. The results show that CVI was higher 30 min post-exercise when 500 ml of water was ingested compared to 50 ml (1.39±0.07 vs. 1.23±0.07; p=0.02) (mean±SEM). Heart rate and blood pressure values were similar in both trials. We conclude that water intake accelerates post-resistance exercise cardiac vagal reactivation. These findings suggest that hydration after resistance exercise might be beneficial for cardiovascular safety in healthy subjects.

Association between different phases of menstrual cycle and body image measures of perceived size, ideal size, and body dissatisfaction.

The association between phases of the menstrual cycle and body image was investigated. 44 university women (M age = 23.3 yr., SD = 4.7) judged their perceived and ideal body size, and body dissatisfaction was calculated at each phase of the menstrual cycle, including premenstrual, menstrual, and intermenstrual. Participants selected one of nine figural drawings ranging from very thin to obese that represented their perceived size and ideal size. Body dissatisfaction was measured as the absolute difference between scores on perceived and ideal figural drawings. During each menstrual phase, anthropometric measures of weight, height, body mass index, circumference of waist and abdomen, and body composition were taken. There were no significant differences in any anthropometric measures between the three menstrual cycle phases. Perceived body size and body dissatisfaction were significantly different between menstrual phases, with the largest perceived body size and highest body dissatisfaction occurring during the menstrual phase. Ideal body size did not differ between menstrual phases, although participants desired a significantly smaller ideal size as compared to the perceived size.