André Lacroix

Female canaries produce eggs with greater amounts of testosterone when exposed to preferred male song

Male birdsong has a great influence in the stimulation of female reproduction. However, female physiological responsiveness to song may depend on the degree of complexity of male song. This is expected because females of iteroparous organisms may increase their fitness by matching their reproductive investment to the predicted value of each reproductive attempt. To the extent that the expression of male ornaments is a signal of male quality, we expect females to increase their investment when paired to highly ornamented males. However, female investment may be cryptic and difficult to detect, such as androgen content in the eggs. In this study, we exposed female canaries (Serinus canaria) to attractive and unattractive song repertoires using a crossover design. As predicted, females invested greater concentrations of testosterone in their eggs when exposed to attractive repertoires than when exposed to unattractive repertoires. This implies that song repertoires convey important information about the reproductive value of a given male and suggests that testosterone deposition in egg yolk may be costly.

Modulation of prolactin but not corticosterone responses to stress in relation to parental effort in a long-lived bird

We tested the hypothesis that parental effort modulates the magnitude of corticosterone and prolactin responses to stress in a long-lived bird, the Black-legged kittiwake (Rissa tridactyla). To do so, we compared corticosterone and prolactin responses to capture/restraint stress between chick-rearing birds and failed breeders (no parental effort). We predicted that (1) the increase in plasma corticosterone levels in response to stress should be lower in chick-rearing birds, (2) the decrease in plasma prolactin levels in response to stress should be lower in chick-rearing birds, and (3) as both sexes care for the chick, there should be no sex difference in the hormonal response to stress. Baseline plasma corticosterone and prolactin levels were higher in chick-rearing birds and were not influenced by body condition. Failed breeders were in better condition than chick-rearing individuals. Corticosterone response to stress was unaffected by parental effort as both chick-rearing and failed birds exhibited a robust corticosterone increase. Prolactin response to stress was however clearly influenced by parental effort: chick-rearing birds showed a modest 9% prolactin decrease whereas in failed birds prolactin concentrations fell by 41%. Body condition did not influence hormonal responses to stress. When facing stressful condition, breeding kittiwakes attenuate their prolactin response to stress while enhancing their secretion of corticosterone. Increasing corticosterone secretion triggers foraging efforts and diminishes nest attendance whereas an attenuation of prolactin response to stress maintains parental behavior. We suggest that this hormonal mechanism facilitates a flexible time-budget that has been interpreted as a buffer against environmental variability.

Seasonal and Fasting-Related Changes in Circulating Gonadal Steroids and Prolactin in King Penguins, Aptenodytes patagonicus

Temporal correlations between reproductive endocrinology and the breeding and molt cycle of the king penguin were studied at the Crozet Islands, in the southern Indian Ocean. This species is unique in having a long cycle (14-15 mo), mainly due to a prolonged fledging period (11 mo), which includes the austral winter. Plasma gonadal steroids and prolactin were at their minimum levels during the prebreeding molt. However, circulating testosterone (males) and estradiol and progesterone (females) were already elevated at the time of arrival at the colony to breed, and the levels peaked during copulation. After laying plasma steroids decreased but remained above basal levels during the following months. Prolactin was low during the sexual phase of the reproductive cycle of both males and females. It reached high plasma concentrations in incubating birds and remained elevated during the whole fledging period. Possible roles for sustained high prolactin levels in winter and spring, which involve the care of the single chick and the prevention of a new cycle, are discussed. The effect of nutritional status of birds on plasma reproductive hormones was also investigated in relation to brief (brooding shifts) and prolonged (incubation spells)fasting. No major effect of food deprivation on plasma hormones was found in incubating and brooding king penguins during normal shifts. However, birds with a low body mass (<9 kg), which progressively mobilized their body protein, presented higher progesterone levels and lower plasma testosterone and prolactin. The decrease in plasma prolactin may be involved in the spontaneous abandon of reproduction previously described in penguins that have depleted their fat stores.

Spontaneous egg or chick abandonment in energy-depleted king penguins: A role for corticosterone and prolactin?

Various exogenous or endogenous factors may induce an emergency response in birds, redirecting current activity towards survival. In fasting, breeding penguins, the achievement of a critical energy depletion was suggested to induce egg abandonment and departure to sea for re-feeding. How such a behavioral shift is hormonally controlled remains unknown. The possible involvement of corticosterone and prolactin was examined by characterizing the nutritional and hormonal states of king penguins at egg abandonment. Further, we tested if these states differ according to whether an egg or a chick is abandoned, and according to the timing of breeding. In every case of abandonment, birds were in phase III fasting characterized by accelerated protein catabolism. However, body condition at egg abandonment was lower in early than in late breeders, suggesting that king penguins are willing to tolerate a larger energy depletion when their potential breeding success is high. At egg and chick abandonment, plasma corticosterone levels were, respectively, increased by 2- and 4-fold, whereas plasma prolactin levels were, respectively, depressed by 3- and 1.4-fold. The increase in plasma corticosterone and the decrease in plasma prolactin could be involved in the control of abandonment by, respectively, stimulating the drive to re-feed and diminishing the drive to incubate or brood. The smaller decrease in prolactin levels and the greater increase in corticosterone levels observed at chick vs egg abandonment suggest that, in addition to nutritionally-related stimuli, tactile or audible stimuli from the egg or chick could intervene in the endocrine control of abandonment.

Yolk androgens in the barn swallow (Hirundo rustica): a test of some adaptive hypotheses.

Maternal effects such as androgen in avian eggs can mediate evolutionary responses to selection, allowing manipulation of offspring phenotype and promoting trans‐generational adaptive effects. We tested the predictions of two adaptive hypotheses that have been proposed to explain female variation in yolk androgen allocation in birds, using the barn swallow Hirundo rustica as a model. We found no support for the first hypothesis proposing that yolk androgen varies as a function of breeding density in order to prepare offspring for different breeding densities. However, we found experimental support for the hypothesis that female yolk androgen allocation depends on mate attractiveness and that it constitutes an example of differential allocation. Females increased the concentration of androgens in their eggs when mated to males with experimentally elongated tails. Female phenotypic quality as measured by arrival date and clutch size was positively related to egg androgen concentration, consistent with the hypothesis that this is a costly investment, constrained by female condition. We found correlative evidence of a direct relationship between egg androgen concentration and performance of offspring as measured by mass increase.

Proteolytic degradation of hemoglobin by endogenous lysosomal proteases gives rise to bioactive peptides: hemorphins.

Hemorphin generation by mice peritoneal macrophages has been recently reported, nevertheless no conclusive data exist to localize clearly the macrophage proteolytic activity implicated in their generation. Because lysosomes are believed to be the main site of degradation in the endocytic pathway, we have studied their potential implication in the generation of hemorphins from hemoglobin. When this protein is submitted to purified rat liver lysosomes, an early generation of hemorphin‐7‐related peptides, detected by a radioimmunoassay, was observed. These peptides seemed to be relatively stable during the first hours of hydrolysis.

Social competition and plasma testosterone profile in domesticated canaries: An experimental test of the challenge hypothesis

The challenge hypothesis predicts that plasma testosterone (T) concentration is high when male-male competitions are high and decreases when males are engaged in paternal care. In monogamous species, T concentration increases at the beginning of the breeding period and decreases after egg laying. According to the challenge hypothesis, increasing competition should also lead to T increase. The aim of our study was to test this hypothesis. In a first experiment, we measured the T profile of domesticated canaries housed with their mate in separated cages without competition. In a second one, we created a competition by housing male and female domestic canaries together (in an aviary) and emphasized this competition by limiting food access. We also studied social status effect. Our results showed no effect of social status in both sexes and no differences in females T concentration. Concerning males, we obtained a clear monogamous T profile from the ones housed in a low competition situation and a polygamous profile from the others housed in high competition situation. Thus, our results support the hypothesis of the plasticity of the mechanisms controlling T concentration according to environmental conditions.

What ecological factors can affect albumen corticosterone levels in the clutches of seabirds? Timing of breeding, disturbance and laying order in rockhopper penguins (Eudyptes chrysocome chrysocome)

Female birds deposit corticosterone into their eggs. Elevated concentrations of this hormone may interfere with the development of their offspring, and mothers should thus regulate corticosterone levels deposited into the eggs adaptively. However, if females are unable to regulate deposition, then the corticosterone concentration in eggs should reflect that in female plasma and should be influenced by stressors to the females. We measured corticosterone levels in the albumen of rockhopper penguins, and assessed their relationship with hatching order, human disturbance and laying date. Rockhopper penguins (Eudyptes chrysocome chrysocome) lay two eggs, of which the second egg (B-egg) is larger and hatches faster than the first egg (A-egg). The chick hatching from the B-egg is also much more likely to survive than its sibling. Albumen corticosterone concentrations were lower in B-eggs. However, as B-eggs contained more albumen than A-eggs, the total corticosterone deposited in the albumen was not significantly different between the two eggs. Daily disturbance by human observers during albumen production did not influence albumen corticosterone levels. Laying date had an effect on total albumen corticosterone through a higher albumen mass. However, we observed a high individual component in the composition of eggs from the same clutch. Thus, more work is required to explore the hypotheses of passive versus active transfer to eggs and to understand the adaptive value of contrary effects on the amount and concentration of corticosterone.

Influence of mating preferences on yolk testosterone in the grey partridge

In precocial bird species, the eggs constitute most of the maternal investment because parents do not feed the young after they hatch. Maternal testosterone in egg yolk influences the embryo’s and chick’s development. Females deposit testosterone in the eggs as a response to the environment experienced during the laying period, including the quality of their mate. To assess the relevance of the female’ sm ate selection on egg characteristics in the grey partridge, Perdix perdix, we tested breeding females in a choice trial where they were allowed to choose between two males. After the choice trials, females were mated either with their preferred male (P group) or with the nonpreferred one (NP group). Although eggs laid by females of the two groups did not differ significantly in mass, females of the P group laid eggs with a higher yolk testosterone concentration than females of the NP group. This study agrees with previous work pointing out that partner attractiveness may play an important role in the transfer of maternally derived egg components. 2010 The Association for the Study of Animal Behaviour. Published by Elsevier Ltd. Maternal effects imply that the environment and the phenotype of the mother affect the progeny’s phenotype (Mousseau & Fox 1998). In oviparous vertebrates, all the resources needed by the embryo to develop must be present in the egg laid by the female. After the eggs are laid, no further adjustments to their components

Plasma steroid and nutrient levels during the active season in wild Testudo horsfieldi

Plasma concentrations of sex steroids (testosterone and progesterone), proteins (total protein and albumin), lipids (phospholipids, cholesterol, and triglycerides), and minerals (calcium and phosphorus) were measured in wild Testudo horsfieldi in Uzbekistan, during the short, 3-month activity period (March-May, 1998). Testosterone concentration in males was highest (52 ng/ml) when they had just emerged from brumation (hibernation) in mid-March, which was also the beginning of the mating period, and fell in April. In females, progesterone peaked in mid-April (at 10 ng/ml), just before ovulation of the first clutches at the end of April and beginning of May. Testosterone levels in females and progesterone levels in males were low (<3 ng/ml) throughout the activity period. In general, the plasma concentrations of proteins, lipids, and phosphorus increased slowly in males, but more rapidly in females, during the activity season. These increases were particularly strong in females in the second half of April, coinciding with the peaks in female hormone levels. The changes in plasma hormones and nutrients reflected the timing of the different behaviours. The four first weeks of above-ground activity (mid-March to mid-April) by males, when they had high testosterone levels, were primarily allocated to fighting other males, courtship, and mating, while females spent much of that time feeding. Thereafter, both sexes concentrated on feeding. Females were probably preparing to ovulate in late-April, when their progesterone levels were highest and when plasma nutrient levels increased considerably.