Andrea Cicconetti

Micro‐CT and PET analysis of bone regeneration induced by biodegradable scaffolds as carriers for dental pulp stem cells in a rat model of calvarial “critical size” defect: Preliminary data

Bone regeneration strategies in dentistry utilize biodegradable scaffolds seeded with stem cells able to induce bone formation. However, data on regeneration capacity of these tissue engineering constructs are still deficient. In this study micro-Computed tomography (micro-CT) and positron emission tomography (PET) analyses were used to investigate bone regeneration induced by two scaffolds [Granular deproteinized bovine bone (GDPB) and Beta-tricalcium phosphate (β-TCP)] used alone or in combination with dental pulp stem cells (DPSC) in a tissue engineered construct implanted in a rat critical calvarial defect. Bone mineral density (BMD) and standard uptake value (SUV) of tracer incorporation were measured after 2, 4, 8, and 12 weeks post-implant. The results showed that: (1) GDPB implants were mostly well positioned, as compared to ß-TCP; (2) GDPB induced higher BMD and SUV values within the cranial defect as compared to ß-TCP, either alone or in combination with stem cells; (3) addition of DPSC to the grafts did not significantly induce an increase in BMD and SUV values as compared to the scaffolds grafted alone, although a small tendency to increase was observed. Thus our study demonstrates that GDPB, when used to fill critical calvarial defects, induces a greater percentage of bone formation as compared to ß-TCP. Moreover, this study shows that addition of DPSC to pre-wetted scaffolds has the potential to ameliorate bone regeneration process, although the set of optimal conditions requires further investigation.

A comparative morphometric analysis of biodegradable scaffolds as carriers for dental pulp and periosteal stem cells in a model of bone regeneration.

AbstractBone regeneration and bone fixation strategies in dentistry utilize scaffolds containing regenerating-competent cells as a replacement of the missing bone portions and gradually replaced by autologous tissues. Mesenchymal stem cells represent an ideal cell population for scaffold-based tissue engineering. Among them, dental pulp stem cells (DPSCs) and periosteal stem cells (PeSCs) have the potential to differentiate into a variety of cell types including osteocytes, suggesting that they can be used with this purpose. However, data on bone regeneration properties of these types of cells in scaffold-based tissue engineering are yet insufficient.In this study, we evaluated temporal dynamic bone regeneration (measured as a percentage of bone volume on the total area of the defect) induced by DPSCs or PeSCs when seeded with different scaffolds to fill critical calvarial defects in SCID Beige nude mice. Two commercially available scaffolds (granular deproteinized bovine bone with 10% porcine collagen and granular &bgr;;-tricalcium phosphate) and one not yet introduced on the market (a sponge of agarose and nanohydroxyapatite) were used. The results showed that tissue-engineered constructs did not significantly improve bone-induced regeneration process when compared with the effect of scaffolds alone. In addition, the data also showed that the regeneration induced by &bgr;;-tricalcium phosphate alone was higher after 8 weeks than that of scaffold seeded with the 2 stem cell lines. Altogether these findings suggest that further studies are needed to evaluate the potential of DPSCs and PeSCs in tissue construct and identify the appropriate conditions to generate bone tissue in critical-size defects.

The surgical approaches to nasopharyngeal angiofibroma.

After a short summary of the aetiopathogenesis, the routes of extension and the diagnostic features of nasopharyngeal angiofibroma, the importance of early diagnosis and careful surgical planning is underlined. In particular the extension and topographic localization allows the choice of the best approach to optimize surgical radicality--the prime concern in the treatment of nasopharyngeal angiofibroma. Different surgical approaches are proposed for the tumour removal according to our experience in 17 patients.

Infratemporal fossa surgery for malignant diseases

SummaryThe improvement in the knowledge of the main anatomical landmarks permits an evolution in the safety of the surgical treatment and a conceptual development of the geometrical anatomico-surgical characteristics of the infratemporal fossa.This conceptual evolution determines surgical and oncological advantages: firstly, improved comprehension of the anatomico-surgical limits of the resection and secondly the safeguarding of the oncological “en-bloc” dissection.The lateral approach of the infratemporal fossa gives a wider exposure of the surgical field, a shorter depth of work, a good control over the vessels and the possibility of carry out a microsurgical transfer.The surgical approaches correspond to the topographical location and the biology of the neoplasm in cases with infratemporal fossa and inferior compartment location the lateral transfacial approach is indicated. In cases with involvement of the superior compartment a lateral transcraniofacial subtemporal approach is necessary in order to remove the skull base. In cases with a neoplastic invasion of the skull base where the dura mater is the anatomical plane free from disease it is necessary to utilize an intradural approach. In patients with a secondary spread into the inferior compartment from the maxilla a combined antero-lateral transfacial approach is indicated. Finally, an orbitomaxillary involvement with secondary spread in the upper compartment of the infratemporal fossa necessitates an antero-lateral transcraniofacial subtemporal subfrontal approach.

Ulcerated pedunculated mass of the maxillary gingiva.

We report a 65-year-old Filipino woman who presentedwith a painful 2 2 1 cm mass arising from the leftmaxillary gingiva causing a poor fit of her maxillary completedenture. The lesion had been present for 2 years, with a rapidincrease in size noted during the preceding 12 months. Thepatient was a nonsmoker, had a noncontributory medicalhistory, and was not taking any medications. No submandib-ular, cervical, or supraclavicular lymphadenopathy was noted.The lesion presented as a soft, exophytic, pedunculated, pol-ypoid, focally ulcerated mass of the left maxillary edentulousalveolar ridge mucosa (Fig. 1). Computerized tomography(CT) imaging showed moderate destruction of bone beneaththe mass and thickening of the left maxillary antral mucosa,interpreted as being secondary to an underlying inflammatoryprocess (Fig. 2,

Radiographic evaluation of the symphysis menti as a donor site for an autologous bone graft in pre-implant surgery

Purpose This study was performed to obtain a quantitative evaluation of the cortical and cancellous bone graft harvestable from the mental and canine regions, and to evaluate the cortical vestibular thickness. Materials and Methods This study collected cone-beam computed tomographic (CBCT) images of 100 Italian patients. The limits of the mental region were established: 5 mm in front of the medial margin of each mental foramen, 5 mm under the apex of each tooth present, and above the inferior mandibular cortex. Cortical and cancellous bone volumes were evaluated using SimPlant software (SimPlant 3-D Pro, Materialize, Leuven, Belgium) tools. In addition, the cortical vestibular thickness (minimal and maximal values) was evaluated in 3 cross-sections corresponding to the right canine tooth (3R), the median section (M), and the left canine tooth (3L). Results The cortical volume was 0.71±0.23 mL (0.27-1.96 mL) and the cancellous volume was 2.16±0.76 mL (0.86-6.28 mL). The minimal cortical vestibular thickness was 1.54±0.41 mm (0.61-3.25 mm), and the maximal cortical vestibular thickness was 3.14±0.75mm(1.01-5.83 mm). Conclusion The use of the imaging software allowed a patient-specific assessment of mental and canine region bone availability. The proposed evaluation method might help the surgeon in the selection of the donor site by the comparison between bone availability in the donor site and the reconstructive exigency of the recipient site.

Metal free, full arch, fixed prosthesis for edentulous mandible rehabilitation on four implants

PURPOSE The goal of this work is to describe an implant-prosthetic protocol for rehabilitation of edentulous mandible, by using a fixed prosthesis made of fiber-reinforced composite material (FRC). The protocol contemplates a minimal invasive surgery and ensures predictable and safe results, with good aesthetic and performance combined to cost savings. METHODS FRC material is used to build the substructure of a prosthetic framework supported by four short implants (5mm long and 4mm wide). The prosthesis substructure is made of Trinia immersed in a matrix of epoxy resin (FRC). It is supplied in milling blocks (pre-cured) for the CAD/CAM (computer-aided design/computer-aided manufacturing) technique. Implants are placed in lower edentulous jaw in position of first molar and canine, each side. Four month after, a resin bar is build based on a stone model, denture teeth are placed and the occlusion is checked. The resin bar and the stone model with milled abutments are scanned and a FRC bar is achieved with the CAD/CAM technique. The teeth are mounted to the substructure trough denture resin. Temporary cementation of framework is achieved on the abutments connected to the implants. CONCLUSION A protocol for a fixed mandibular implant-prosthetic rehabilitation is described. The protocol contemplates a minimal invasive surgery and ensures predictable and safe results, with good aesthetic and performance combined to cost savings. In addition, this technique allows performing basic surgery also in presence of atrophy.

An anatomical radiographic evaluation of the posterior portion of the mandible in relation to autologous bone harvest procedures.

Abstract The purpose of the study was to evaluate the course of the mandibular canal and the thickness of the vestibular cortical plate in the posterior region of the mandible in relation to autologous bone harvest procedures. The study was performed on a cohort of 30 cone-beam computed tomography hemimandible images. For each hemimandible, the course of the mandibular canal and the thickness of the vestibular cortical plate have been evaluated in 4 regions: the retromolar region, the second molar region, the first molar region, and the second premolar region. The analyzed variables show a characteristic trend: the thickness of the cortical vestibular plate and the horizontal distance of the canal from the cortical vestibular plate are higher in the second molar region specifically in the area bordering on the retromolar region. In fact, the maximum thickness reaches the average value of 3.46 mm on 30 hemimandibles for slice (SD, 0.56 mm; range, 2.36–4.83 mm), and the horizontal distance reaches the average value of 6.06 mm on 30 hemimandibles for slice (SD, 1.34 mm; range, 3.65–9.27 mm); both variables decrease in more distal slices of the retromolar region. The vertical distance of the canal from the cortical crest shows the average value of 14.25 mm on 22 slices of the second molar and retromolar regions (SD of average values, 1.03 mm; range of average values, 12.92–16.25 mm; range of absolute values, 7.11–22.92 mm) exactly in regions potentially suitable for procedures of bone harvest (second molar and retromolar regions).