Andrea Martini

Butyric acid glycerides in the diet of broiler chickens: effects on gut histology and carcass composition

Abstract Aim of the study was to verify the effects of butyric acid glycerides, as a supplemental ingredient in the diet, on live performance of broiler chickens and on the morphology of their small intestine, since short chain fatty acids are known as selective protection factors against intestinal microbial parasites, potent growth promoters of the gut wall tissues, also in terms of immune modulation response. An experiment was carried out on 150 Ross 308 female chickens, allotted to 5 treatments, over a 35 d ays period: the control, with soybean oil as the energy supplement, and 4 treatments with increasing amounts (0.2, 0.35, 0.5, 1% mixed feed) of a mixture of butyric acid glycerides (mono-, di- and tri-glycerides). Treated animals showed a higher live weight at slaughtering (P<0.05) with a better feed conversion rate. The carcase characteristics were not influenced, but the small intestine wall resulted slightly modified with shorter villi, longer microvilli (P<0.01) and larger crypts depth in jejunum (P<0.01), only with lowest concentration of the supplement (0.2%). It is concluded that butyric acid glycerides are an efficient supplement to broilers’ diets, deserving particular attention as a possible alternative to antimicrobial drugs, which have been banned in Europe.

Quantitative and qualitative analysis of the effectiveness of a mechanical method for the isolation of preantral follicles from ovine ovaries.

The preantral follicles are the major source of oocytes and its utilization has been investigated as an important tool to store large numbers of female gametes for further utilization in reproductive programs. The aim of the present study was to perform quantitative and qualitative analyses of the efficacy of a mechanical method for isolating of preantral follicles from the ovaries of fetuses and from nonpregnant and pregnant ewes, using as reference the population of preantral follicles in situ. In the isolation method the ovaries were cut into fragments in the tissue chopper. Then, the suspension was filtered through nylon mesh filters. The number of isolated follicles per ovary was 1655, 4735 and 4770, respectively, for the fetus, nonpregnant ewe and pregnant ewe. The number of in situ preantral follicles per ovary was 32961, 16627 and 17794, respectively, for the fetus, nonpregnant ewe and pregnant ewe. The follicle recovery rate (number of isolated preantral follicles/number of in situ preantral follicles x 100) was higher in adult ewes (26 and 28%, respectively, for nonpregnant and pregnant ewes) than in fetuses (5%). Histological analysis showed that very few preantral follicles (less than 0.26% in situ and 0.46% after the isolation procedure) were degenerated. In conclusion, this study showed that a mechanical method could be used effectively to isolate a large number of intact ovine preantral follicles. In the future, with improvements in culture systems, the isolation of a great number of oocytes enclosed in preantral follicles will make a valuable contribution to the rare breeds and endangered species, agricultural efficiency and basic research in folliculogenesis.

Dietary extra-virgin olive oil rich in phenolic antioxidants and the aging process: long-term effects in the rat

The aim of the present work was to verify whether extra-virgin olive oil, a food naturally containing phenolic antioxidants, has the potential to protect from the pro-aging effects of a high-calorie diet. Male rats were fed from age 12 months to senescence a high-calorie diet containing either corn oil (CO), or extra-virgin olive oil with high (H-EVOO) or low (L-EVOO) amounts of phenols. The prolonged high fat intake led to obesity, liver lipid degeneration and insulin resistance, which were not counteracted by high phenol intake. No difference in overall survival was found at the end of the experiment in the animals treated with H-EVOO compared to the other groups. However, we did detect a protective effect of olive oil on some age-related pathologies and on blood pressure, of which the former was associated with the antioxidant content. Concomitantly, a decrease in DNA oxidative damage in blood cells and plasma TBARS and an increase in liver superoxide dismutase were detected following H-EVOO consumption. Thus, although olive oil phenols cannot reverse the detrimental effects of a prolonged intake of high amounts of fat, improving the quality of olive oil in terms of antioxidant content can be beneficial.

Productive performances of Maremmana young bulls reared following organic rules and slaughtered at 18 and 24 months of age

Fourteen Maremmana young bulls fed on pastures supplemented with mixed hay and concentrates, according to an organic method, were slaughtered at 18 and 24 months of age to evaluate in vita and post-mortem performances. At slaughter, carcass yield was determined and carcass evaluation according to EU rules was made. Physico-chemical characteristics of Longissimus thoracis (LT) and Caput longum triceps brachii (TB) muscles were determined. No differences for both carcass yield and quality were found between the two age groups. Animals of 24 months of age presented lighter and more yellow meat as well as higher hue angle and drip loss. TB muscle showed lower protein content and higher shear force, moisture, total lipids and ash than LT. The latter presented a higher percentage of C15:0iso, whereas TB showed higher values of C18:2n6, C20:3n6, C20:4n6, C18:3n3 and C22:5n3. Maremmana animals, irrespective of age, showed a low percentage of saturated fatty acids, good PUFA/SFA ratio and a great amount of unsaturated fatty acids of the n3 and n6 series, as well as a favorable atherogenicity and thrombogenicity indexes.

Response surface analyses of the associative effects of lucerne hay, wheat straw and maize gluten feed on growing lambs

Abstract Twenty-seven male lambs were fed nine complete diets based on 40% concentrate and either wheat straw (S), lucerne hay (H), maize gluten feed (G) or their associations. The growth performances and the comparative slaughter technique of lambs were used as the means to study the possible associative effect between S, H and G. The response surface analysis (RSA) model was used to statistically analyse all the growth and slaughter data. As was expected, the presence of S in the place of H in the ration had a depressing effect on the dry matter intake ( P P P As a conclusion, it is observed that the statistical model represented by the RSA adopted in this work was a very useful and precise means to study the associative effects between ingredients of complete diets. This kind of approach deserves further work to be validated.

Reduction of risk of dying from tobacco-related diseases after quitting smoking in Italy

Aims and Background The aims of this paper are to compute the risks of dying of ischemic heart disease (IHD), lung cancer (LC), stroke, and chronic obstructive pulmonary disease (COPD) for Italian smokers by gender, age and daily number of cigarettes smoked, and to estimate the benefit of stopping smoking in terms of risk reduction. Methods Life tables by sex and smoking status were computed for each smoking-related disease based on Italian smoking data, and risk charts with 10-year probabilities of death were computed for never, current and former smokers. Results Men aged 45-49 years, current smokers, have a 8, 10, 3 and 1 in 1,000 chance of dying of IHD, LC, stroke and COPD, respectively, whereas women with the same characteristics have a 2, 6, 3 and 1 in 1,000 chance, respectively, for all smokers combined, i.e., independent of the smoking intensity. The risk reduction rates from quitting smoking are remarkable: a man who quits smoking at 45-49 years can reduce the risk of dying of IHD, LC, stroke and COPD in the next 10 years by 43%, 53%, 57% and 55%, respectively; a woman by 49%, 49%, 59% and 57%, respectively. Conclusions Estimates of risk reduction by quitting smoking are useful to provide a sounder scientific basis for public health messages and clinical advice.

Life Gain in Italian Smokers Who Quit

This study aims to estimate the number of life years gained with quitting smoking in Italian smokers of both sexes, by number of cigarettes smoked per day (cig/day) and age at cessation. All-cause mortality tables by age, sex and smoking status were computed, based on Italian smoking data, and the survival curves of former and current smokers were compared. The more cig/day a man/woman smokes, and the younger his/her age of quitting smoking, the more years of life he/she gains with cessation. In fact, cessation at age 30, 40, 50, or 60 years gained, respectively, about 7, 7, 6, or 5, and 5, 5, 4, or 3 years of life, respectively, for men and women that smoked 10–19 cig/day. The gain in life years was higher for heavy smokers (9 years for >20 cig/day) and lower for light smokers (4 years for 1–9 cig/day). Consistently with prospective studies conducted worldwide, quitting smoking increases life expectancy regardless of age, gender and number of cig/day. The estimates of the number of years of life that could be gained by quitting smoking, when computed specifically for a single smoker, could be used by physicians and health professionals to promote a quit attempt.

Breast cancer mortality trends in Italy by region and screening programme, 1980–2008

Objectives To describe trends in breast cancer mortality in 14 Italian regions representing 90% of the Italian population, and in the country overall, in relation to the diffusion of organized or opportunistic breast cancer screening programmes. Settings: Breast cancer mortality figures from 1980–2008 in Italy and 14 Italian regions were retrieved from the National Statistics Institute. Methods Estimated annual percent change (EAPC) was calculated for three time-periods (1980–1989, 1990–1999, 2000–2008). To estimate the age-adjusted and age-specific EAPC, trends in mortality based on annual data were examined using a log-linear regression model, and the year of death as a regressor variable. Results In the period 1980–1989 an increasing trend was observed in Italy overall (EAPC = +1.1%; 95% confidence interval [95%CI]: + 0.9%; + 1.3%) and in the 14 regions. In the second decade a decreasing trend was recorded both overall in Italy (EAPC = −1.6%; 95CI:−1.8%;−1.4%) and in all the regions. Between 2000–2008 a slightly decreasing trend was observed in Italy overall (EAPC = −0.8%; 95%CI:−1.0%;−0.5%). A steeper and earlier decrease compared with the rest of Italy was observed in 1990–1999 in Liguria (EAPC = −3.1%; 95%CI:−4.0%,−2.1%), Lombardy (EAPC = −2.3%; 95%CI:−2.8%;−1.9%), and Tuscany (EAPC = −3.0%; 95%CI:−3.7%;−2.2%), mainly due to the 55–64 age−group. There were significant EAPCs between −4.1% and −3.7% in these three regions, 2.5–3.0 times greater than in the rest of Italy (EAPC = −1.4%). Conclusions This earlier decline in three Italian regions could be partially attributable to local organized screening programmes implemented earlier than in the rest of Italy, and to opportunistic screening that became more common in 1990s.

Efficiency and costs of the health management in an organic dairy farm where we use unconventional medicines

Abstract The EU organic regulation explicitly promote the use of unconventional therapies, like homoeopathy and phytotherapy. The aim of the present study was to investigate the efficiency and the costs of these treatment methods. From December 2006 to September 2008, we analyzed the data recorded in an organic dairy farm where the animals are normally treated by classical unicistic homeopathy and phytotherapy, and only when indispensable, by allophaty, antiparasitic drugs, surgery and vaccines. The use of homeopathy resulted to be predominant in comparison with the others treatments. Besides, our trial showed that homeopathy and phytotherapy could be used to treat, with good outcomes, the majority of diseases that occur in a dairy cattle farm, even if, sometimes, conventional medicines have to be used. The costs for unconventional treatments are very low in comparison with conventional ones. This will allow the spreading of unconventional medicines in the Italian organic farms.

Possible development of organic production in a mountain area of the Florence province in Tuscany

The organic production technique is a significant innovation for both process and product aspects of the agricultural sector. In less favoured areas, organic production contributes to develop the potential revitalisation of the production sector and also supports the application of endogenous development models, thus favouring sustainable development both on socio‐economic and ecological bases. The paper focuses on a case study about the possible growth of organic production in a mountain area of the Florence province, in Tuscany. The organic and non‐organic agricultural activities of the area are illustrated, with regards to the whole territorial organisation and the need to maintain agricultural activities for territorial defence. The potential development of the organic product supply is described as a result of a direct survey on the major distribution channels. The main reasons for the delayed expansion of the organic production are outlined in a direct survey on the farms of the area.