Andréa Thomaz Soccol

Estudo comparativo da biocompatibilidade da submucosa intestinal porcina e pericárdio bovino usados como enxertos na veia cava de cães

PURPOSE To compare the biocompatibility of the bovine pericardium and the small intestine submucosa (SIS) when used to repair a created defect in the inferior vena cava of dogs. METHODS Sixteen male mongrel dogs were submitted to a midline laparotomy incision. An elliptical segment (1.5 x 3.0 cm) of the inferior vena cava, below the renal veins, was excised. In 8 dogs, the A group, a patch of porcine small bowel submucosa was used to repair the defect. In the 8 remaining dogs, the B group, a bovine pericardium was implanted in the vena cava. On the 30th post-operative day an ultrasound was performed in order to identify stenosis. The euthanasia was accomplished in the 40th post-operative day. Stenosis of the vein was observed in 1 dog of A group and in 2 of the B group. RESULTS Partial thrombosis with collateral circulation was evidenced in 1 dog of the bovine pericardium group. A moderated chronic inflammatory process was evidenced in both groups. Microscopic evaluation, regarding endothelization of the implant, collagen deposition, smooth muscle growth, was similar in both groups. CONCLUSION SIS proved to be a functional graft patch for possessing local vena cava remodeling and its biocompatibility was similar to bovine pericardium.

Use of small intestine submucosa as ureteral allograft in pigs

PURPOSE The aim of the present study was to evaluate the biocompatibility of small intestine submucosa (SIS) in the reconstruction of the ureter in swine. MATERIALS AND METHODS An experimental study was performed in 10 half-breed pigs weighing between 20 and 30 K, in which a previously prepared segment of SIS measuring approximately 2.0 cm was implanted in the upper third part of the right ureter. RESULTS Of the 10 operated animals, one died 14 days after the surgery due to a dehiscence on the suture line of the implanted graft. The remaining 9 animals were submitted to ultrasound examination of the urinary tract and were sacrificed on the 40th postoperative day. The macroscopic evaluation showed no calculus, incrustation, fistula, abscesses or adhesions in the ureters with the graft. Microscopic evaluation with hematoxylin-eosin and Sirius red showed in the experimental area (graft) the presence of urothelium in 100% of the cases, collagen in 100% of the cases, and smooth muscle layer in 87.5% of the animals. In the area adjacent to the graft (proximal and distal), we observed 92.86% of urothelium, 42.86% of collagen and 71.43% of smooth muscle. In the contralateral ureter, it was found 100% of urothelium and smooth muscle and just 11.11% of collagen. The microscopic analysis of the kidneys whose ureters received the graft of SIS evidenced congestion in 55.55%, pelvic edema in 66.66% and interstitial nephritis in 77.78%. Hydronephrosis was present in 33.33% and chronic pyelonephritis in 44%. Only 1 animal presented total absence of glomerulus in the renal parenchyma. CONCLUSION The SIS graft behaved as a biological tissue support, allowing the regeneration of the urothelium and smooth muscle grow, despite of chronic inflammatory process.

Utilização da submucosa de intestino delgado porcino como retalho para aumento da capacidade vesical em cães

Objetivo: Avaliar a biocompatibilidade de um enxerto xenogenico de SID como meio de ampliacao da capacidade vesical. Metodos: Oito caes mesticos foram submetidos a laparotomia e abertura da bexiga por incisao mediana de 3cm em sua face ventral. Para aumento da capacidade vesical, bem como sua reconstituicao, foi interposto um segmento de submucosa porcina, fixando-o a parede vesical. No 30°dia de pos-operatorio os animais foram sacrificados e procedeu-se a analise macroscopica. As pecas foram entao encaminhadas para fixacao, coloracao e analise microscopica. Resultados: Nao se observou seroma, hematoma, abscesso, fistula, deiscencia, aderencias, litiase e a nao incorporacao do enxerto. Microscopicamente observou-se em todos os casos uma proliferacao do urotelio que recobriu toda a superficie do enxerto, alem da presenca de fibras musculares lisas no local do implante. Verificou-se proliferacao conjuntiva, principalmente as custas de colageno imaturo do tipo III e reacao inflamatoria cronica em todos os animais. A proliferacao vascular foi acentuada e a reabsorcao da membrana foram tambem observadas. Conclusao: Asubmucosa de intestino delgado porcino participou como um substrato para a regeneracao da bexiga e pode ser uma futura alternativa na reconstrucao do trato urinario.

Comparação entre os bioenxertos de hidroxiapatita de cálcio e submucosa de intestino delgado porcino no preenchimento de defeitos ósseos criados em mandíbula de ratos

AIM: The aim of this study was to evaluate the bone formation in surgically created defects of rabbit mandibles by synthetic hydroxyapatite of calcium compared to small Intestine Submucosa. MATERIAL AND METHOD: 24 mices lineage Wisthar-Furth were used. A bony defect of 0,75 cm x 1,5 cm in mandibular ramus was accomplished in all animals. The hydroxyapatite implants were placed on the left hemimandiblein groupI, small Intestine submucosa in group II, and the right served as control. The euthanasia was accomplished in the 40° postoperative day, it was proceeded the macroscopic and histological analysis. RESULTS: medium length in millimeters of the hemimandibless in the hydroxyapatite group was of 3,75, in the small intestine submucosa 3,03 and the control group was of 2,63 (p: 0,022). Histomorphometry study reaveled new bone grown in 76,64% of the total area in hydroxyapatite group (p: 0,022). In Small Intestinal submucosa group new bone grown in 63,64% do total (p: 0,0022). DISCUSSION: satisfactory bone integration was observed of the synthetic hydroxyapatite in that experimental model. Small intestinal submucosa cause osteoinduction CONCLUSAO: using hydroxyapatite of calcium resulted in formation of significantly larger volume frations of new bone when compared to small intestinal submucosa group.

Defect repair in rat mandible with hydroxyapatite cement comparad to small intestine submucosa

AIM The aim of this study was to evaluate the bone formation in surgically created defects of rabbit mandibles by synthetic hydroxyapatite of calcium compared to small Intestine Submucosa. MATERIAL AND METHOD 24 mice lineage Wisthar-Furth were used. A bony defect of 0,75 cm x 1,5 cm in mandibular ramus was accomplished in all animals. The hydroxyapatite implants were placed on the left hemimandiblein group I, small Intestine submucosa in group II, and the right served as control. The euthanasia was accomplished in the 40 degrees postoperative day, it was proceeded the macroscopic and histological analysis. RESULTS medium length in millimeters of the hemimandibless in the hydroxyapatite group was of 3,75, in the small intestine submucosa 3,03 and the control group was of 2,63 (p: 0,022). Histomorphometry study revealed new bone grown in 76,64% of the total area in hydroxyapatite group (p: 0,022). In Small Intestinal submucosa group new bone grown in 63,64% do total (p: 0,0022). DISCUSSION satisfactory bone integration was observed of the synthetic hydroxyapatite in that experimental model. Small intestinal submucosa cause osteoinduction CONCLUSION using hydroxyapatite of calcium resulted in formation of significantly larger volume fractions of new bone when compared to small intestinal submucosa group.

Spasmodic Dysphonia: Description of the Disease and Associated Neurologic Disorders

Introduction: Spasmodic dysphonia (SD) is a problem that affects speech and vocalization, one of the most devastating disorders of oral communication. It is characterized by vocal quality tensaestrangulada, harshly and / or interspersed with abrupt vocal attack and a great tension in the vocal tract. The etiology of spasmodic dysphonia is unclear. Some authors point to psychogenic causes, neurological or even unknown. Objective: To assess the prevalence of muscular dystonias and other neurological symptoms in patients with ED. Method: A retrospective study of 10 cases with diagnosis of ED for symptoms and neurological disorders associated. Results: There was a significant predominance of the disease in females (9:1). The average age of onset of symptoms was 32 years, ranging between 14 and 60 years. The mean disease duration was 10 years. Among the patients, 87.5% had a diagnosis of disorders of movement made by a neurologist, including orofacial dystonias (50%), essential tremor (50%) and spastic paraparesis (12%). Conclusion: The presence of movement disorders followed almost all cases of spasmodic dysphonia. More studies are needed to clarify the pathophysiological basis of disease.