Andrei Kovalenko

THE YEAST TWO-HYBRID SCREENING TECHNIQUE AND ITS USE IN THE STUDY OF PROTEIN-PROTEIN INTERACTIONS IN APOPTOSIS

The yeast two-hybrid technique provides a general approach for cloning cDNAs merely by exploiting the ability of their encoded proteins to bind to a protein of interest. The technique therefore offered a useful access to the analysis of the mechanisms of cell death at the initial stage of their study, when only a few of the proteins involved and very little about their mode of action were known. Conversely, the knowledge of cell death mechanisms gained by this technique provided a useful insight into both the potential and the limitations of this technique.