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Dive into the research topics where Andreia Possatti da Rocha is active.

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Featured researches published by Andreia Possatti da Rocha.


Journal of Alzheimer's Disease | 2013

Caffeine Consumption Prevents Memory Impairment, Neuronal Damage, and Adenosine A2A Receptors Upregulation in the Hippocampus of a Rat Model of Sporadic Dementia

Janaína Espinosa; Andreia Possatti da Rocha; Fernanda Nunes; Marcelo S. Costa; Vanessa Schein; Vanessa Kazlauckas; Eduardo Kalinine; Diogo O. Souza; Rodrigo A. Cunha; Lisiane O. Porciúncula

Intracerebroventricular (icv) streptozotocin (STZ) administration induces pathological and behavioral alterations similar to those observed in Alzheimers disease (AD) and is thus considered an experimental model of sporadic AD. Since caffeine (an adenosine receptor antagonist) and selective antagonists of adenosine A2A receptors modify the course of memory impairment in different amyloid-β-based experimental models of AD, we now tested the impact of caffeine on STZ-induced dementia and associated neurodegeneration in the hippocampus as well as on the expression and density of adenosine receptors. Adult male rats received a bilateral infusion of saline or STZ (3 mg/kg, icv), which triggered memory deficits after four weeks, as gauged by impaired object recognition memory. This was accompanied by a reduced NeuN immunoreactivity in the hippocampal CA1 region and an increased expression and density of adenosine A2A receptors (A2AR), but not A1R, in the hippocampus. Caffeine consumption (1 g/L in the drinking water starting 2 weeks before the STZ challenge) prevented the STZ-induced memory impairment and neurodegeneration as well as the upregulation of A2AR. These findings provide the first demonstration that caffeine prevents sporadic dementia and implicate the control of central A2AR as its likely mechanism of action.


Endocrine-related Cancer | 2010

The RET polymorphic allele S836S is associated with early metastatic disease in patients with hereditary or sporadic medullary thyroid carcinoma

Débora Rodrigues Siqueira; Mirian Romitti; Andreia Possatti da Rocha; Lucieli Ceolin; Camila Meotti; Aline Albeche Farias Estivalet; Márcia Khaled Punãles; Ana Luiza Maia

The possible role of RET variants in modifying the natural course of medullary thyroid carcinoma (MTC) is still a matter of debate. Here, we investigate whether the RET variants L769L, S836S, and G691S/S904S influence disease presentation in hereditary or sporadic MTC patients. One hundred and two patients with hereditary MTC and 81 patients with sporadic MTC attending our institution were evaluated. The frequencies of RET polymorphisms in hereditary MTC were as follows: L769L, 17.3%; S836S, 7.95%; and S904S/G691S, 18.2%. No associations were observed between these polymorphisms and pheochromocytoma, hyperparathyroidism, lymph node, or distant metastasis. However, patients harboring the S836S variant were younger than those without this allele (17±8.2 vs 28.6±14.4 years, P=0.01), suggesting that these patients had metastases at a young age. Accordingly, the cumulative frequency of local and/or distant metastases as estimated by Kaplan-Meier curves showed that lymph node and distant metastases occurred earlier in patients harboring the S836S variant (P=0.003 and P=0.026 respectively). The S836S allele frequency was higher in sporadic MTC patients than in controls (10.5 vs 3.1%, P=0.01). Individuals harboring the S836S variant were younger (38.6±13.3 vs 48.5±16.7 years, P=0.02) and showed a higher percentage of lymph node and distant metastases (P=0.02 and P=0.04 respectively). Kaplan-Meier estimates of lymph node and distant metastases yielded distinct curves for patients with or without the S836S allele (P=0.002 and P=0.001 respectively). Additional analyses using a COX regression model showed that the S836S variant was independently associated with metastatic disease (hazard ratio 2.82 (95% confidence interval 1.51-5.26), P=0.001). In conclusion, the RET S836S variant is associated with early onset and increased risk for metastatic disease in patients with hereditary or sporadic MTC.


Thyroid | 2008

Clinical and Oncological Features of Children and Young Adults with Multiple Endocrine Neoplasia Type 2A

Márcia Khaled Punãles; Andreia Possatti da Rocha; Camila Meotti; Jorge Luiz Gross; Ana Luiza Maia

BACKGROUND RET genotype analysis allows identification of asymptomatic carriers at risk of developing medullary thyroid carcinoma (MTC). However, there is still controversy regarding the ideal timing and extent of prophylactic thyroidectomy due to the wide spectrum of clinical presentation. Surveillance of a large number of young patients is crucial to advance our understanding of the natural course of the disease. This study aimed to describe the clinical presentation, oncological features, and treatment outcome of children and young adults harboring RET mutations followed at our institution from 1997 to 2007. METHODS Forty-one individuals aged < or =25 years from 17 independent multiple endocrine neoplasia type 2A kindred were studied. Twenty-one individuals presented with thyroid nodules at diagnosis, and 20 were disease free at physical examination. RESULTS Preoperative basal calcitonin levels were elevated in 85.7% of patients with clinical disease and in 54.5% of asymptomatic carriers. Thyroid ultrasonography (US) showed one or more nodules in 69.0% of the patients. A positive correlation between age at surgery and tumor-node-metastasis (TNM) stages was observed (p < 0.001). None of the patients under 15 years of age presented lymph node or distant metastasis. After a follow-up of 4.4 +/- 1.4 years all asymptomatic patients were disease free based on physical examination, cervical US, and undetectable serum calcitonin levels. In the group of patients with clinical disease, 47.6% have persistent disease (follow-up of 12.0 +/- 5.9 years). Indeed, palpable thyroid nodule at diagnosis was significantly associated with persistent disease (p < 0.001, odds ratio [OR] 1.9, 95% confidence interval [CI 95%] 1.27-2.87). Of note, none of the patients who presented lymph node metastasis at diagnosis were cured by surgical intervention (p < 0.001, OR 5.0, CI 95% 1.45-17.0). CONCLUSION Our data show a time-dependent MTC progression. The presence of a palpable thyroid nodule and lymph node metastasis at diagnosis was associated with persistent or recurrent disease after surgical procedure.


Arquivos Brasileiros De Endocrinologia E Metabologia | 2005

Carcinoma diferenciado de tireóide: avaliação inicial e acompanhamento

Lenara Golbert; Simone Magagnin Wajner; Andreia Possatti da Rocha; Ana Luiza Maia; Jorge Luiz Gross

ABSTRACT Differentiated Thyroid Carcinoma: Initial Evaluation and Follow-up. Thyroid carcinoma accounts for roughly 1% of all new malignant disea-ses. Of these, at least 94% are differentiated thyroid carcinoma (DTC),either papillary thyroid carcinoma or follicular thyroid carcinoma. Pa-tients with DTC are usually considered as having a good prognosis, 80%of patients are cured, 20% will develop loco-regional recurrence and 5-10% distant metastasis. However, the disease may have an aggressivecourse in some patients. The identification of these patients has a majorimpact in the clinical management of DTC. Several prognostic factorsand classification will be addressed, as well the most useful tests forpatient’s follow-up. (Arq Bras Endocrinol Metab 2005;49/5:701-710)K e y w o r d s : Differentiated thyroid carcinoma; Initial evaluation; Follow-u p CARCINOMAS DIFERENCIADOS DE TIREOIDE O CÂNCER DE TIREOIDE e a neoplasia maligna mais frequente do sistemaendocrinologico, apesar de ser uma patologia relativamente rara,sendo responsavel por aproximadamente 1% dos novos casos de doencamaligna (1). A cada ano, nos EUA, surgem 14.000 novos casos, e ocorrem1.100 mortes decorrentes do carcinoma diferenciado da tireoide (2). NoBrasil, estes numeros sao proporcionais, ocorrendo 66 novos casos em cada100.000 habitantes por ano (3).As neoplasias da tireoide sao classificadas de acordo com o tipohistologico em adenoma folicular, carcinoma papilar, carcinoma folicular ecarcinoma anaplasico ou indiferenciado. A maioria dos tumores tireoi-


Arquivos Brasileiros De Endocrinologia E Metabologia | 2007

Polimorfismos genéticos: implicações na patogênese do carcinoma medular de tireóide

Andreia Possatti da Rocha; Patrícia Künzle Ribeiro Magalhães; Ana Luiza Maia; Léa Maria Zanini Maciel

Medullary thyroid carcinoma (MTC) is a rare malignant neoplasia, which may occur on sporadic form or on a hereditary basis. Germ line mutations in the RET proto-oncogene is responsible for hereditary MTC. However, most MTC occur in individuals without family history where the pathogenesis is still unclear. Single nucleotide polymorphisms (SNPs) of the RET gene have been described in the general population as well as in patients with MTC. Even though these allelic variants do not seem to confer any transforming activity to the tyrosine kinase domain of the RET protein, cumulative studies suggest that they could modify disease susceptibility and clinical phenotype in patients with sporadic or hereditary MTC. Polymorphisms located in exons 11 (G691S), 13 (L769L), 14 (S836S), and 15 (S904S) seem to be over-represented in sporadic MTC patients from American and European countries. Here, we discuss the results obtained in different studies as well as describe the frequency of RET polymorphisms in Brazilian patients with sporadic MTC.


Arquivos Brasileiros De Endocrinologia E Metabologia | 2004

Carcinoma medular de tireóide: aspectos moleculares, clínico-oncológicos e terapêuticos

Márcia Khaled Punãles; Andreia Possatti da Rocha; Jorge Luiz Gross; Ana Luiza Maia

ABSTRACT Medullary Thyroid Carcinoma: Clinical and Oncological Features andTreatment. Medullary carcinoma of the thyroid (MTC) may be sporadic or may occuron a hereditary basis. Hereditary MTC can occur either alone – familialMTC (FMTC) – or as the thyroid manifestation of multiple endocrine neo-plasia type 2 (MEN 2) syndromes (MEN 2A and MEN 2B) or other forms.Germ-line mutations in R E T cause MEN 2. Genetic testing, now available,forms the basis for MTC screening procedures. In the past few years, sev-eral genotype-phenotype correlations have focused on the relationshipbetween specific mutations and different MEN 2 syndrome variants. Differ-ences in dimerization induction intensities are a reasonable explanation forthe phenotypes resulting from mutations of the different cysteines. Here wedescribed the molecular mechanisms, diagnose and treatment as well asour experience on the management of this rare form of thyroid cancer. (Arq Bras Endocrinol Metab 2004;48/1:137-146)Keywords: MTC;


Arquivos Brasileiros De Endocrinologia E Metabologia | 2009

Identification of occult metastases of medullary thyroid carcinoma by calcitonin measurement in washout fluid from fine needle aspiration of cervical lymph node.

Débora Rodrigues Siqueira; Andreia Possatti da Rocha; Márcia Khaled Punãles; Ana Luiza Maia

Medullary thyroid carcinoma (MTC) may occur sporadically or as a manifestation of an autosomal-dominant inherited syndrome, the multiple endocrine neoplasia type 2. DNA-based RET genotype analysis gained worldwide acceptance in the identification of asymptomatic gene carrier. MTC synthesize and secrete calcitonin, a well established tumor marker and postoperative level of serum calcitonin, indicates whether residual disease was left behind and whether reintervention is necessary. However, management is difficult when routine imaging studies for MTC are negative. This paper brings a report of an illustrative case of a patient with MTC diagnosed by molecular screening, who persisted with detectable levels of serum calcitonin after surgical procedure. After 48 months, an increase in serum calcitonin impelled us to investigate the disease focus. Cervical-US and calcitonin measurement in washout fluid from fine needle aspiration was successfully used to identify MCT metastasis in a lymph node, allowing appropriated reintervention and illustrating the potential clinical applicability of this method.


Progress in Neuro-psychopharmacology & Biological Psychiatry | 2018

Sex differences in the effects of pre- and postnatal caffeine exposure on behavior and synaptic proteins in pubescent rats

Cássia Sallaberry; Ana Paula Ardais; Andreia Possatti da Rocha; Maurício Felisberto Borges; Gabriela T. Fioreze; Sabrina Mioranzza; Fernanda Bordignon Nunes; Natália Pagnussat; Paulo Henrique S. Botton; Lisiane O. Porciúncula

&NA; Few studies have addressed the effects of caffeine in the puberty and/or adolescence in a sex dependent manner. Considering that caffeine intake has increased in this population, we investigated the behavioral and synaptic proteins changes in pubescent male and female rats after maternal consumption of caffeine. Adult female Wistar rats started to receive water or caffeine (0.1 and 0.3 g/L in drinking water; low and moderate dose, respectively) during the active cycle at weekdays, two weeks before mating. The treatment lasted up to weaning and the offspring received caffeine until the onset of puberty (30–34 days old). Behavioral tasks were performed to evaluate locomotor activity (open field task), anxious‐like behavior (elevated plus maze task) and recognition memory (object recognition task) and synaptic proteins levels (proBDNF, BDNF, GFAP and SNAP‐25) were verified in the hippocampus and cerebral cortex. While hyperlocomotion was observed in both sexes after caffeine treatment, anxiety‐related behavior was attenuated by caffeine (0.3 g/L) only in females. While moderate caffeine worsened recognition memory in females, an improvement in the long‐term memory was observed in male rats for both doses. Coincident with memory improvement in males, caffeine increased pro‐ and BDNF in the hippocampus and cortex. Females presented increased proBDNF levels in both brain regions, with no effects of caffeine. While GFAP was not altered, moderate caffeine intake increased SNAP‐25 in the cortex of female rats. Our findings revealed that caffeine promoted cognitive benefits in males associated with increased BDNF levels, while females showed less anxiety. Our findings revealed that caffeine promotes distinct behavioral outcomes and alterations in synaptic proteins during brain development in a sex dependent manner. HighlightsFemale pubescent rats were more responsive to caffeine‐induced hyperlocomotion.Caffeine worsened recognition memory in female pubescent rats.Recognition memory was improved by caffeine in male pubescent rats.Anxiety‐related behavior was attenuated by caffeine in female pubescent rats.Both pro‐ and BDNF increased in the hippocampus after caffeine treatment in males.


Frontiers in Aging Neuroscience | 2016

Cognitive Intervention As an Early Non-pharmacological Strategy in Alzheimer's Disease: A Translational Perspective

Sarah Wehle Gehres; Andreia Possatti da Rocha; Antoine Leuzy; Cássio Morais Loss; Giordano G. Viola; Eduardo Rigon Zimmer

Brain amyloid-β (Aβ) accumulation is currently considered the main causative pathophysiological event in Alzheimers disease (AD) (Hardy and Higgins, 1992; Karran et al., 2011). Importantly, this process is thought to precede the onset of AD clinical symptoms by more than two decades, indicating that early therapeutic strategies prior to symptomatology offer the best chance of success. In line with this, there is growing attention being paid to the concept of cognitive reserve (CR) (Stern et al., 1994; Stern, 2002). CR concept is based on extensive epidemiological data indicating that those with higher lifetime levels of social, physical, and cognitive engagement have a lower risk of developing dementia despite the presence of brain pathology (Fratiglioni et al., 2004; Nithianantharajah and Hannan, 2009). Recently, cognitive intervention (CI)—such as cognitive training (Bahar-Fuchs et al., 2013), cognitive stimulation (Woods et al., 2012), and cognitive rehabilitation (Clare et al., 2003)—has emerged as a potential non-pharmacological strategy for the treatment and prevention of AD (Gates and Sachdev, 2014). Although based upon distinct theoretical constructs, these CI strategies are frequently not distinguished in clinical trials.


Arquivos Brasileiros De Endocrinologia E Metabologia | 2007

Polimorfismos genticos: implicaes na patognese do carcinoma medular de tireide

Andreia Possatti da Rocha; Patrícia Künzle Ribeiro Magalhães; Ana Luiza Maia; Léa Maria Zanini Maciel

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Ana Luiza Maia

Universidade Federal do Rio Grande do Sul

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Jorge Luiz Gross

Universidade Federal do Rio Grande do Sul

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Lenara Golbert

Universidade Federal do Rio Grande do Sul

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Débora Rodrigues Siqueira

Universidade Federal do Rio Grande do Sul

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Lisiane O. Porciúncula

Universidade Federal do Rio Grande do Sul

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Simone Magagnin Wajner

Universidade Federal do Rio Grande do Sul

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Aline Albeche Farias Estivalet

Universidade Federal do Rio Grande do Sul

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