Andrew Barton

Potential improvements to statistical downscaling of general circulation model outputs to catchment streamflows with downscaled precipitation and evaporation

An existing streamflow downscaling model (SDM(original)), was modified with the outputs of a precipitation downscaling model (PDM) and an evaporation downscaling model (EDM) as additional inputs, for improving streamflow projections. For this purpose, lag 0, lag 1 and lag 2 outputs of PDM were individually introduced to SDM(original) as additional inputs, and then it was calibrated and validated. Performances of the resulting modified models were assessed using Nash-Sutcliffe efficiency (NSE) during calibration and validation. It was found that the use of lag 0 precipitation as an additional input to SDM(original) improves NSE in calibration and validation. This modified streamflow downscaling model is called SDM(lag0_preci). Then lag 0, lag 1 and lag 2 evaporation of EDM were individually introduced to SDM(lag0_preci) as additional inputs and it was calibrated and validated. The resulting models showed signs of over-fitting in calibration and under-fitting in validation. Hence, SDM(lag0_preci) was selected as the best model. When SDM(lag0_preci) was run with observed lag 0 precipitation, a large improvement in NSE was seen. This proved that if precipitation produced by the PDM can accurately reproduce the observations, improved precipitation predictions will produce better streamflow predictions.

A benign approach to the preparation of freshwater bryozoan statoblasts for scanning electron microscopy (SEM) imaging

Abstract Several different species of freshwater Bryozoa, belonging to the genera Plumatella, Rumarcanella and Fredericella, were detected within the Northern Mallee Pipeline (NMP) system in Victoria, Australia, that required definitive identification. These organisms produce asexual buds called statoblasts, with valves composed of sclerotised chitin that bear minute micro-ornamentations of considerable taxonomical significance. Imaging and analysis of these distinctive micro-ornamentations using scanning electron microscopy (SEM) is often employed for species identification. Meticulous preparation of statoblast samples is therefore required that necessitates the removal of adhering debris, dehydration and drying—whilst mitigating specimen damage and distortion. This technical note describes an approach whereby each of these three steps have been individually designed to be as benign as possible, using mild detergent/sonication to remove debris, a gradual and gentle dehydration procedure using ethanol, and critical point drying. For the overall process, these methods are chosen to optimise control and to minimise the use of harsh and hazardous chemicals.

Identification of five bryozoan species in the Northern Mallee Pipeline, Australia

Abstract Five species of freshwater bryozoans were identified in the Northern Mallee Pipeline (NMP) system in Victoria, Australia, from statoblast morphology using scanning electron microscopy. The five species were Plumatella emarginata, Fredericella australiensis, Plumatella reticulata, Rumarcanella minuta and Fredericella sultana. The latter three of these have never previously been reported as occurring on the Australian continent and samples have been preserved for safekeeping at Victoria University. The statoblasts were isolated from samples collected as intact colonies from filters and membranes at a number of different locations.