Andrew E. Bowkett

Recent Captive-Breeding Proposals and the Return of the Ark Concept to Global Species Conservation

Captive breeding for reintroduction is a much discussed tool in the management of endangered species. Although a number of high-profile case studies illustrate the critical role captive breeding can play in preventing species extinction (e.g., Stanley Price 1989; Kleiman & Rylands 2002; Cade & Burnham 2003), the available historical evidence indicates most reintroduction attempts failed (Griffith et al. 1989; Wolf et al. 1996). Over the past decade, the number of reintroduction programs and the body of scientific literature documenting these efforts has increased dramatically (Seddon et al. 2007), but the role of captive breeding remains controversial (e.g., Jule et al. 2008) despite well-documented successes. In its simplest form, the role of captive breeding and reintroduction in conservation is analogous to Noah’s ark. Species threatened with extinction are maintained in captivity, as if aboard an ark escaping the flood, until those factors threatening their existence are removed and they can be returned to the wild. This analogy has been widely used in the popular and scientific literature (Durrell 1976; Soule et al. 1986; Balmford et al. 1995). Here I focus on the extent to which the importance of captive breeding and reintroduction as a conservation strategy is reflected in planning and policy, particularly by the world’s zoos and aquaria. I tracked policy and opinion in the zoo and conservation biology literature and found a general decline in emphasis on captive breeding since the early 1990s until the recent recommendations for large-scale captive breeding pertaining to hundreds of species from certain taxonomic groups. It is worth considering whether these new proposals are open to the same criticisms as their predecessors and whether they

Genetic testing of dung identification for antelope surveys in the Udzungwa Mountains, Tanzania

Dung counts are frequently employed to infer abundance of antelope species in African forests, but the accuracy of dung identification has rarely been tested. We used non-invasive genetic methods to test the accuracy of both field identification and morphometrics for identifying dung samples collected in the Udzungwa Mountains, Tanzania. Species identity was established by sequencing part of the mitochondrial control region from faecal DNA. Field identification was found to be correct in only 58–76% of cases depending on the observer. Discriminant analysis of dung pellet length correctly classified 80% of samples but a larger reference sample size is needed before using this method to classify dung of unknown origin. The results of this study illustrate the potential inaccuracy of dung counts as a monitoring tool for sympatric forest antelope species when the probability of correct identification is unknown. We recommend molecular testing of species identity during forest antelope surveys before conclusions are drawn on the basis of other identification methods.

Tracking the amphibian pathogens Batrachochytrium dendrobatidis and Batrachochytrium salamandrivorans using a highly specific monoclonal antibody and lateral-flow technology

The fungus Batrachochytrium dendrobatidis (Bd) causes chytridiomycosis, a lethal epizootic disease of amphibians. Rapid identification of the pathogen and biosecurity is essential to prevent its spread, but current laboratory‐based tests are time‐consuming and require specialist equipment. Here, we describe the generation of an IgM monoclonal antibody (mAb), 5C4, specific to Bd as well as the related salamander and newt pathogen Batrachochytrium salamandrivorans (Bsal). The mAb, which binds to a glycoprotein antigen present on the surface of zoospores, sporangia and zoosporangia, was used to develop a lateral‐flow assay (LFA) for rapid (15 min) detection of the pathogens. The LFA detects known lineages of Bd and also Bsal, as well as the closely related fungus Homolaphlyctis polyrhiza, but does not detect a wide range of related and unrelated fungi and oomycetes likely to be present in amphibian habitats. When combined with a simple swabbing procedure, the LFA was 100% accurate in detecting the water‐soluble 5C4 antigen present in skin, foot and pelvic samples from frogs, newts and salamanders naturally infected with Bd or Bsal. Our results demonstrate the potential of the portable LFA as a rapid qualitative assay for tracking these amphibian pathogens and as an adjunct test to nucleic acid‐based detection methods.

A new population of the Critically Endangered Aders’ duiker Cephalophus adersi confirmed from northern coastal Kenya

Aders’ duiker Cephalophus adersi is a small antelope endemic to the coastal forests of east Africa. Threatened by habitat loss and hunting, the species is categorized as Critically Endangered on the IUCN Red List. Until recently Aders’ duiker was known to persist only on Zanzibar, Tanzania, and in the Arabuko-Sokoke Forest National Reserve, Kenya. However, in 2004 a sighting of a single individual was reported from the Dodori forest in northern coastal Kenya, raising the possibility that the species survives elsewhere. Subsequently, an opportunistic camera-trap survey was conducted in September and October 2008 to establish the occurrence of Aders’ duiker in Kenyan coastal forests north of the Tana River. One hundred and fifty six images of Aders’ duikers were obtained from 12 of 28 camera-trap sites (46 of 358 camera-trap days), confirming the existence of a population of Aders’ duiker in the Boni–Dodori forest both inside and outside the National Reserves. In addition, we sighted individuals of the species on three occasions. The relatively high encounter rates per unit effort compared to similar data from Arabuko-Sokoke forest suggest the Boni–Dodori population is significant. Initial surveys of the local Awer community revealed that Aders’ duiker is well known by the name guno . These findings significantly improve the conservation prospects for Aders’ duiker and highlight the need for greater research and management efforts in the poorly known Boni–Dodori forest.

Genetic Patterns in Forest Antelope Populations in the Udzungwa Mountains, Tanzania, as Inferred from Non-Invasive Sampling

ABSTRACT As for many tropical regions, the evolutionary and demographic status of antelope populations in the Udzungwa Mountains, Tanzania, are poorly resolved. We employed genetic information from 618 faecal samples to assess the status of forest antelope species in terms of their distribution, intraspecific diversity and population subdivision within the Udzungwa landscape. Most species were detected in the majority of forest fragments, except for Philantomba monticola. Phylogenetic analyses were consistent with traditional taxonomy with the exception of Cephalophus harveyi which was paraphyletic with respect to C. natalensis. There was strong support for three C. harveyi mtDNA clades within the Udzungwa Mountains although nuclear genetic variation did not partition strongly with these maternal lineages. Significant partitioning of genetic variation between sampling areas was detected for all species except the endangered C. spadix. Overall, our results demonstrate the value of non-invasive genetic sampling in studying the distribution and evolution of rarely observed species.