Andrew M. Bradbury
Los Alamos National Laboratory
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Publication
Featured researches published by Andrew M. Bradbury.
mAbs | 2015
Andrew M. Bradbury; Csaba Kiss; Sara D'Angelo; Fortunato Ferrara; Leslie A. Naranjo; Tiziano Gaiotto
Only a small fraction of the antibodies in a traditional polyclonal antibody mixture recognize the target of interest, frequently resulting in undesirable polyreactivity. Here, we show that high-quality recombinant polyclonals, in which hundreds of different antibodies are all directed toward a target of interest, can be easily generated in vitro by combining phage and yeast display. We show that, unlike traditional polyclonals, which are limited resources, recombinant polyclonal antibodies can be amplified over one hundred million-fold without losing representation or functionality. Our protocol was tested on 9 different targets to demonstrate how the strategy allows the selective amplification of antibodies directed toward desirable target specific epitopes, such as those found in one protein but not a closely related one, and the elimination of antibodies recognizing common epitopes, without significant loss of diversity. These recombinant renewable polyclonal antibodies are usable in different assays, and can be generated in high throughput. This approach could potentially be used to develop highly specific recombinant renewable antibodies against all human gene products.
Proceedings of SPIE | 2011
Tiziano Gaiotto; Hau B. Nguyen; Jaemyeong Jung; Gnana S. Gnanakaran; Jurgen G. Schmidt; Geoffrey S. Waldo; Andrew M. Bradbury; Peter M. Goodwin
We are exploring the use of fluorogen-activating proteins (FAPs) as reporters for single-molecule imaging. FAPs are single-chain antibodies selected to specifically bind small chromophoric molecules termed fluorogens. Upon binding to its cognate FAP the fluorescence quantum yield of the fluorogen increases giving rise to a fluorescent complex. Based on the seminal work of Szent-Gyorgyi et al. (Nature Biotechnology, Volume 26, Number 2, pp 235-240, 2008) we have chosen to study two fluorogen-activating single-chain antibodies, HL1.0.1-TO1 and H6-MG, bound to their cognate fluorogens, thiazole orange and malachite green derivatives, respectively. Here we use fluorescence correlation spectroscopy to study the photophysics of these fluorescent complexes.
Archive | 2011
Andrew M. Bradbury; Geoffrey S. Waldo; Csaba Kiss
Archive | 2011
Andrew M. Bradbury
Archive | 2008
Andrew M. Bradbury; Csaba Kiss; Geoffrey S. Waldo
Archive | 2002
Andrew M. Bradbury; Ahmet Zeytun; Geoffrey S. Waldo
Archive | 2010
Andrew M. Bradbury; Geoffrey S. Waldo; Csaba Kiss; Devin W. Close
Archive | 2006
Andrew M. Bradbury
Archive | 2017
Andrew M. Bradbury; Csaba Kiss; Geoffrey S. Waldo
Archive | 2011
Carolyn R. Bertozzi; John Kehoe; Andrew M. Bradbury