Angela Corrêa Freitas-Almeida

Occurrence of Staphylococcus and enteropathogens in soft cheese commercialized in the city of Rio de Janeiro, Brazil

Aims: To investigate the presence of Staphylococcus aureus, enteropathogenic Escherichia coli (EPEC), Aeromonas spp. and Yersinia spp. in soft cheese commercialized in Rio de Janeiro, Brazil.

Genome Sequence of the Emerging Pathogen Aeromonas caviae

Aeromonas caviae is a Gram-negative, motile and rod-shaped facultative anaerobe that is increasingly being recognized as a cause of diarrhea in children. Here we present the first genome sequence of an A. caviae strain that was isolated as the sole pathogen from a child with profuse diarrhea.

Interactions of clinical and environmental Aeromonas isolates with Caco-2 and HT29 intestinal epithelial cells

Aim:  Evaluation of adherence and invasion of Aeromonas spp. to human colon carcinoma cell lines Caco‐2 and HT29 and assessment of cytotoxic activity.

Incidence and antimicrobial resistance of enteropathogens isolated from an integrated aquaculture system.

Aims:  To evaluate an integrated aquaculture system, microbiological analyses of water used in this system were carried out and the incidence and antimicrobial resistance of enteropathogens were determined in the related ecosystem.

Association of Aeromonas caviae polar and lateral flagella with biofilm formation.

Aim:  To evaluate the association of the polar and lateral flagella with biofilm formation on plastic surfaces in 76 Aeromonas caviae strains isolated from environment (lagoon water), food (vegetables, fish and cheese) and human source (faeces).

First report of the emerging zoonotic agent Wohlfahrtiimonas chitiniclastica isolated from a retail frozen chicken in Rio de Janeiro, Brazil

Wohlfahrtiimonas chitiniclastica is an emerging zoonotic bacterium commensally living in larvae of particular flies. It has been associated with human and animal infections but never isolated from food. In the present study, a whole chicken carcass was rinsed in buffered peptone water which was then inoculated into BHI and the growth plated onto selective medium. Species identification was performed by MALDI–TOF MS. Those bacteria identified as W. chitiniclastica were subjected to 16S rRNA sequencing for confirmation and MEGA software was used to obtain their phylogenetic position. The findings of this study raise concerns regarding the abattoir, transport and stock practices of frozen meat carcasses and should be of interest with regard to microbiology, entomology and food production.

Lectin-binding properties of Aeromonas caviae strains

The cell surface carbohydrates of four strains of Aeromonas caviae were analyzed by agglutination and lectin-binding assays employing twenty highly purified lectins encompassing all sugar specificities. With the exception of L-fucose and sialic acid, the sugar residues were detected in A. caviae strains. A marked difference, however, in the pattern of cell surface carbohydrates in different A. caviae isolates was observed. Specific receptors for Tritricum vulgaris (WGA), Lycopersicon esculentum (LEL) and Solanum tuberosum (STA) (D-GlcNAc-binding lectins) were found only in ATCC 15468 strain, whereas Euonymus europaeus (EEL, D-Gal-binding lectin) sites were present exclusively in AeQ32 strain, those for Helix pomatia (HPA, D-GalNAc-binding lectin) in AeC398 and AeV11 strains, and for Canavalia ensiformes (Con A, D-Man-binding lectin) in ATCC 15468, AeC398, AeQ32 and AeV11 strains, after bacterial growing at 37°C. On the other hand, specific receptors for WGA and EEL were completely abrogated growing the bacteria at 22°C. Binding studies with 125I- labeled lectins from WGA, EEL and Con A were performed. These assays essentially confirmed the selectivity, demonstrated in the agglutination assays of these lectins for the A. caviae strains.

Avaliação Microbiológica, Detecção e Susceptibilidade Aos Antimicrobianos de Potenciais Enteropatógenos Das Famílias Enterobacteriaceae e Vibrionaceae em Mexilhões Perna Perna Coletados Na Praia de Itaipu, Niterói-Rj

Marcelly Miranda Aybal Jayme, Jorge Goncalo Caldeira Nunes de Matos, Alessandro Sales, Monica Conceicao NunesCarvalho, Mara Lucia Penna Queiroz, Angela Correa de Freitas-Almeida, Fabio Vieira Araujo.Avaliacao Microbiologica, Deteccao e Susceptibilidade Aos Antimicrobianos de Potenciais Enteropatogenos Das Familias Enterobacteriaceae e Vibrionaceae em Mexilhoes Perna Perna Coletados Na Praia de Itaipu, Niteroi-Rj. In: Anais do 12o Congresso Latinoamericano de Microbiologia e Higiene de Alimentos MICROAL 2014 [= Blucher Food Science Proceedings, num.1, vol.1]. Sao Paulo: Editora Blucher, 2014. DOI 10.5151/foodsci-microal-185 Avaliacao Microbiologica, Deteccao e Susceptibilidade Aos Antimicrobianos de Potenciais Enteropatogenos Das Familias Enterobacteriaceae e Vibrionaceae em Mexilhoes Perna Perna Coletados Na Praia de Itaipu, Niteroi-RJ